Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U8327_RS14885 Genome accession   NZ_CP141765
Coordinates   3129854..3131239 (-) Length   461 a.a.
NCBI ID   WP_053296155.1    Uniprot ID   -
Organism   Escherichia coli strain HYM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3124854..3136239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8327_RS14850 (U8327_14850) mutT 3125303..3125701 (+) 399 WP_000736038.1 8-oxo-dGTP diphosphatase MutT -
  U8327_RS14855 (U8327_14855) yacG 3125789..3125986 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  U8327_RS14860 (U8327_14860) zapD 3125996..3126739 (-) 744 WP_001194720.1 cell division protein ZapD -
  U8327_RS14865 (U8327_14865) coaE 3126739..3127359 (-) 621 WP_001269513.1 dephospho-CoA kinase -
  U8327_RS14870 (U8327_14870) - 3127384..3127428 (+) 45 WP_120795372.1 protein YacM -
  U8327_RS14875 (U8327_14875) guaC 3127584..3128627 (+) 1044 WP_001217337.1 GMP reductase -
  U8327_RS14880 (U8327_14880) hofC 3128662..3129864 (-) 1203 WP_053296154.1 protein transport protein HofC -
  U8327_RS14885 (U8327_14885) pilB 3129854..3131239 (-) 1386 WP_053296155.1 type II secretion system protein GspE Machinery gene
  U8327_RS14890 (U8327_14890) ppdD 3131249..3131689 (-) 441 WP_324700844.1 prepilin peptidase-dependent pilin -
  U8327_RS14895 (U8327_14895) nadC 3131892..3132785 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  U8327_RS14900 (U8327_14900) ampD 3132873..3133424 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  U8327_RS14905 (U8327_14905) ampE 3133421..3134275 (+) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  U8327_RS14910 (U8327_14910) aroP 3134318..3135691 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50668.43 Da        Isoelectric Point: 6.5405

>NTDB_id=837575 U8327_RS14885 WP_053296155.1 3129854..3131239(-) (pilB) [Escherichia coli strain HYM1]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDVGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRLQQGESIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLEMHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=837575 U8327_RS14885 WP_053296155.1 3129854..3131239(-) (pilB) [Escherichia coli strain HYM1]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTAGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGTCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACACTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCTGCAGCAAGGGGAGTCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAATGCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Haemophilus influenzae 86-028NP

40.127

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.052

83.514

0.401
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Vibrio cholerae strain A1552

47.15

83.731

0.395
  pilF Neisseria gonorrhoeae MS11

44.56

83.731

0.373
  pilB Acinetobacter baylyi ADP1

44.301

83.731

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilB Acinetobacter baumannii D1279779

44.125

83.08

0.367
  pilF Thermus thermophilus HB27

41.032

88.286

0.362