Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JLC71_RS00195 Genome accession   NZ_AP024094
Coordinates   20410..22116 (-) Length   568 a.a.
NCBI ID   WP_200916685.1    Uniprot ID   A0A7I8E6K8
Organism   Jeongeupia sp. HS-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 15410..27116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLC71_RS00165 (JHS3_00310) - 15816..16568 (-) 753 WP_200916679.1 GNAT family N-acetyltransferase -
  JLC71_RS00170 - 16658..16843 (-) 186 WP_200916680.1 DNA gyrase inhibitor YacG -
  JLC71_RS00175 (JHS3_00320) zapD 16840..17598 (-) 759 WP_200916681.1 cell division protein ZapD -
  JLC71_RS00180 (JHS3_00330) coaE 17667..18281 (-) 615 WP_200916682.1 dephospho-CoA kinase -
  JLC71_RS00185 (JHS3_00340) pilD 18284..19162 (-) 879 WP_200916683.1 A24 family peptidase Machinery gene
  JLC71_RS00190 (JHS3_00350) pilC 19162..20385 (-) 1224 WP_200916684.1 type II secretion system F family protein Machinery gene
  JLC71_RS00195 (JHS3_00360) pilB 20410..22116 (-) 1707 WP_200916685.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JLC71_RS00200 (JHS3_00370) - 22232..23488 (-) 1257 WP_200916686.1 HlyC/CorC family transporter -
  JLC71_RS00205 (JHS3_00380) - 23542..24189 (-) 648 WP_200916687.1 inner membrane protein YpjD -
  JLC71_RS00210 (JHS3_00390) ffh 24430..25779 (+) 1350 WP_200916688.1 signal recognition particle protein -
  JLC71_RS00215 (JHS3_00400) rpsP 25880..26131 (+) 252 WP_200916689.1 30S ribosomal protein S16 -
  JLC71_RS00220 (JHS3_00410) rimM 26190..26672 (+) 483 WP_200916690.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61873.98 Da        Isoelectric Point: 5.1059

>NTDB_id=83449 JLC71_RS00195 WP_200916685.1 20410..22116(-) (pilB) [Jeongeupia sp. HS-3]
MSTTPSISGLARLLVQHGRLSDTDAETLQSTAATNKAPFIAQLLQSGKMNAKEVAEFSAQAFGYPLLDLEQIDTSYIPQG
VLDTKVMQAQRIVPLFKRGTKLFIGISDITNLQAAEEVRFQTGAQIEPIVVEDNKLVKLLDHLLEASGATLKNLEMDESE
LEISGGDPEPQNDTANIDVDDAPVVKYLQKILLDAINANASDIHFEPYEKYYRIRYRQDGILREIAQPPLAIKEKIASRI
KVISKLDIAEKRVPQDGRMKLVLSKSRAIDFRVSTLPTLHGEKIVMRILDPTSATLGVDALGYEPDQKAALLNAVTRPYG
MVLVTGPTGSGKTVSLYTCLNILNEPGTNISTAEDPAEINLPGVNQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRDL
ETADIAIKAAQTGHMVFSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLARRLCSHCKAPADIPNEALLRAGFTE
GDLDGSWTPYKAIGCDLCKDTGYKGRVGIYQVMPISDEINRIIMRNGTAIDVADQARLEGVRDLRQSGLLKVKQGMTSLE
EVMAVTNE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=83449 JLC71_RS00195 WP_200916685.1 20410..22116(-) (pilB) [Jeongeupia sp. HS-3]
ATGTCCACTACCCCCTCGATATCCGGACTCGCTCGCCTGCTCGTCCAGCACGGCCGGCTCAGCGACACCGATGCAGAAAC
GCTGCAAAGCACGGCGGCCACCAACAAGGCACCGTTCATCGCGCAACTGCTGCAAAGCGGCAAAATGAATGCCAAGGAGG
TCGCCGAATTCAGCGCCCAGGCCTTCGGCTACCCACTGCTCGACCTCGAACAGATCGACACCAGCTACATCCCTCAGGGT
GTGCTTGATACCAAGGTGATGCAGGCGCAGCGGATCGTGCCGCTGTTCAAACGCGGCACCAAGCTGTTCATTGGCATCTC
CGACATCACCAATTTGCAGGCCGCCGAGGAAGTCCGCTTCCAGACCGGTGCGCAGATCGAGCCCATCGTTGTCGAAGACA
ACAAGCTCGTCAAACTGCTCGATCATTTGCTCGAAGCATCCGGCGCCACGCTGAAAAACCTCGAGATGGACGAGTCCGAG
CTCGAAATCAGTGGTGGCGACCCCGAGCCGCAGAACGACACCGCGAATATCGATGTCGACGATGCACCAGTGGTCAAATA
CCTGCAGAAAATCCTGCTGGATGCCATCAACGCCAACGCATCCGACATCCACTTTGAACCATATGAAAAGTACTACCGGA
TTCGCTACCGCCAAGATGGCATCTTGCGCGAAATCGCCCAGCCACCGCTGGCGATCAAGGAAAAAATCGCCTCGCGGATC
AAGGTCATCTCCAAGCTCGACATCGCCGAAAAACGCGTCCCGCAGGATGGCCGGATGAAACTGGTGCTGTCGAAAAGCCG
GGCCATCGATTTCCGGGTTTCGACGCTACCTACGCTGCACGGCGAAAAAATCGTCATGCGGATCCTCGACCCGACCTCTG
CGACGCTGGGGGTCGATGCGCTGGGTTACGAGCCGGATCAAAAAGCCGCCCTGCTCAACGCGGTGACTCGGCCTTACGGC
ATGGTGCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTATCGCTGTATACCTGCCTGAACATTCTCAACGAACCCGG
CACGAATATTTCGACCGCCGAAGACCCGGCGGAAATCAACCTGCCCGGCGTTAATCAGGTCAACGTCAACGACAAGGCCG
GACTGACCTTCTCCGCCGCGCTCAAGGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGTGAAATCCGCGACCTG
GAAACCGCCGACATCGCGATCAAGGCCGCGCAAACCGGCCACATGGTGTTCTCGACGCTACACACCAACGACGCACCGGG
CACCCTGACCCGCCTGCTGAATATGGGCGTAGCGCCGTTCAACGTCGCCAGCTCGGTGATCCTGATCACCGCGCAGCGTC
TGGCACGGCGACTGTGCTCGCACTGCAAGGCCCCGGCGGATATCCCCAACGAAGCGTTGCTGCGCGCAGGCTTCACCGAA
GGCGATCTGGACGGCAGCTGGACCCCCTACAAGGCGATCGGCTGCGATCTATGCAAGGACACCGGCTACAAAGGCCGGGT
TGGCATCTATCAGGTCATGCCGATCTCTGATGAAATCAACCGCATCATCATGAGAAACGGCACGGCGATCGACGTTGCCG
ACCAGGCGCGCCTGGAAGGCGTGCGCGACCTGCGGCAATCCGGGCTGCTGAAAGTCAAACAGGGCATGACCTCGCTTGAG
GAAGTCATGGCCGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7I8E6K8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.517

100

0.558
  pilF Neisseria gonorrhoeae MS11

55.694

98.944

0.551
  pilB Acinetobacter baylyi ADP1

54.72

100

0.551
  pilB Legionella pneumophila strain ERS1305867

53.274

99.472

0.53
  pilB Vibrio cholerae strain A1552

47.276

100

0.474
  pilB Vibrio campbellii strain DS40M4

45.841

99.472

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

46.085

98.944

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

92.958

0.387
  pilF Thermus thermophilus HB27

37.258

100

0.373

Multiple sequence alignment