Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QLH64_RS21285 Genome accession   NZ_CP125961
Coordinates   4940774..4942474 (+) Length   566 a.a.
NCBI ID   WP_057450869.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain R401     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4935774..4947474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLH64_RS21265 (QLH64_21265) - 4935869..4938802 (+) 2934 WP_057450871.1 DUF748 domain-containing protein -
  QLH64_RS21270 (QLH64_21270) - 4938804..4939100 (+) 297 WP_057450870.1 DUF2845 domain-containing protein -
  QLH64_RS21275 (QLH64_21275) - 4939428..4939781 (-) 354 WP_003205664.1 BON domain-containing protein -
  QLH64_RS21280 (QLH64_21280) - 4940127..4940549 (-) 423 WP_053163361.1 pilin -
  QLH64_RS21285 (QLH64_21285) pilB 4940774..4942474 (+) 1701 WP_057450869.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QLH64_RS21290 (QLH64_21290) pilC 4942477..4943694 (+) 1218 WP_057450868.1 type II secretion system F family protein Machinery gene
  QLH64_RS21295 (QLH64_21295) pilD 4943696..4944565 (+) 870 WP_057450867.1 A24 family peptidase Machinery gene
  QLH64_RS21300 (QLH64_21300) coaE 4944565..4945188 (+) 624 WP_057450866.1 dephospho-CoA kinase -
  QLH64_RS21305 (QLH64_21305) yacG 4945185..4945391 (+) 207 WP_057450865.1 DNA gyrase inhibitor YacG -
  QLH64_RS21310 (QLH64_21310) - 4945388..4945603 (-) 216 WP_013694234.1 hypothetical protein -
  QLH64_RS21315 (QLH64_21315) - 4945667..4946353 (-) 687 WP_057450864.1 energy-coupling factor ABC transporter permease -
  QLH64_RS21320 (QLH64_21320) - 4946391..4946804 (-) 414 WP_057450863.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62701.70 Da        Isoelectric Point: 5.3203

>NTDB_id=833711 QLH64_RS21285 WP_057450869.1 4940774..4942474(+) (pilB) [Pseudomonas brassicacearum strain R401]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPSGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNNGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=833711 QLH64_RS21285 WP_057450869.1 4940774..4942474(+) (pilB) [Pseudomonas brassicacearum strain R401]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGTCGCATCCCCTTGGTGAGCTATCTGGTGCAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAGATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAACCCAACCAAGCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCATGCCCTGCCGCTGTGGCGGCGCGGCAACAAGCTGTACGTGGGCATCTCCGA
CCCAACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTTTTCGAGTCCAGCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGATATCGAATCGATCGACGACACCAGGCAAGACTCCATCGCCGGCCAGGACACCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCAATCAAGGGTGGCTCTTCCGACTTGCACTTCGAGCCCTATGAAAAGATCTACCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGAGAAGTAGCCAGGCCGCCGATCCAACTGGCCAACCGTATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAACCCCAGGATGGGCGCCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCATCCAGCG
CACAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGA
CTGATCCTGGTGACCGGCCCAACCGGCTCGGGCAAGACCGTGTCGCTCTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAATCCGCGCCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTACAGAACATGGGCATTCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATTGCCCAGCGGC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAGGCCATCGAAATTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCTCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAATAACGGCTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGCGCATCGGGGCTGCTCAAAGTGATGCAGGGGGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.304

100

0.583
  pilB Acinetobacter baumannii D1279779

57.848

100

0.58
  pilB Legionella pneumophila strain ERS1305867

55.379

100

0.555
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Thermus thermophilus HB27

36.042

100

0.36