Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QNH97_RS24185 Genome accession   NZ_CP125957
Coordinates   5523776..5525476 (+) Length   566 a.a.
NCBI ID   WP_283554234.1    Uniprot ID   -
Organism   Pseudomonas sp. G2-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5518776..5530476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH97_RS24165 (QNH97_24165) - 5518943..5521876 (+) 2934 WP_283554231.1 DUF748 domain-containing protein -
  QNH97_RS24170 (QNH97_24170) - 5521878..5522174 (+) 297 WP_283554232.1 DUF2845 domain-containing protein -
  QNH97_RS24175 (QNH97_24175) - 5522509..5522862 (-) 354 WP_025215523.1 BON domain-containing protein -
  QNH97_RS24180 (QNH97_24180) - 5523135..5523551 (-) 417 WP_283554233.1 pilin -
  QNH97_RS24185 (QNH97_24185) pilB 5523776..5525476 (+) 1701 WP_283554234.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QNH97_RS24190 (QNH97_24190) pilC 5525479..5526696 (+) 1218 WP_283554235.1 type II secretion system F family protein Machinery gene
  QNH97_RS24195 (QNH97_24195) pilD 5526698..5527567 (+) 870 WP_283554236.1 A24 family peptidase Machinery gene
  QNH97_RS24200 (QNH97_24200) coaE 5527580..5528203 (+) 624 WP_283554237.1 dephospho-CoA kinase -
  QNH97_RS24205 (QNH97_24205) yacG 5528200..5528406 (+) 207 WP_135847217.1 DNA gyrase inhibitor YacG -
  QNH97_RS24210 (QNH97_24210) - 5528403..5528618 (-) 216 WP_003185533.1 hypothetical protein -
  QNH97_RS24215 (QNH97_24215) - 5528682..5529368 (-) 687 WP_283554238.1 energy-coupling factor ABC transporter permease -
  QNH97_RS24220 (QNH97_24220) - 5529600..5530226 (+) 627 WP_030140261.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62769.88 Da        Isoelectric Point: 5.5675

>NTDB_id=833666 QNH97_RS24185 WP_283554234.1 5523776..5525476(+) (pilB) [Pseudomonas sp. G2-4]
MNDIALSGLTKQLVLAELITEQSAQQAYQQAQRNRMPLISYLVQNKLVQSRRVAEIASEHFGVALLDLNSLDKDSQPIGL
VSEKLLRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDSKQESIGGMDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRVRTDGILREVAKPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKNKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCTHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLQKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=833666 QNH97_RS24185 WP_283554234.1 5523776..5525476(+) (pilB) [Pseudomonas sp. G2-4]
ATGAATGACATCGCCCTCAGCGGTTTGACCAAGCAACTGGTGCTGGCCGAACTGATCACCGAGCAAAGTGCGCAGCAGGC
GTACCAACAGGCCCAGCGCAATCGCATGCCCCTGATCAGCTATCTGGTGCAGAACAAACTGGTGCAGAGCCGCCGGGTTG
CTGAAATTGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAGGACTCTCAGCCCATCGGGCTG
GTCAGCGAAAAGCTGCTGCGTCAACATCACGCGCTGCCGCTGTGGCGGCGCGGCAACAAGTTGTTCGTGGGCATCTCCGA
CCCGACCAATCACCAAGCCATCAATGACATTCAATTCAGTACCGGCCTCACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAGAAATTCTTCGAATCCAGCAGTACAGGCCTGGAAGGCATGGGCGACGTCGATCTCGACGGC
CTGGACATCGAATCGATCGACGATTCCAAGCAGGAGTCCATCGGCGGCATGGACACCGACGACGCCCCGGTGGTGCGCTT
CGTCAACAAGATGTTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGGTGCGCACCGACGGCATCCTTCGGGAAGTGGCCAAGCCGCCGATCCAACTGGCCACCCGCATCGCCGCCCGCCTC
AAGGTCATGGCCAGCCTCGATATTTCCGAACGGCGCAAACCCCAGGATGGACGGCTGAAAATGCGCCTGTCGAAAAACAA
ATCCATCGACTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCATCCAGTG
CGCAAATGGGCATCGATGCCCTGGGCTATGAACCGGACCAGAAAGACCTGTACATGGCGGCCCTCAAGCAGCCACAGGGG
CTGATCCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTGGA
CATCAATATTTCCACCGCCGAAGACCCAGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GGCTGGATTTTGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACGCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATCCCTGGCTTCAACATCGCCACCGCGGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCACCCATTGCAAGAAAGCCATCGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGTACATTCACGATCTATGAGCCGGTCGGTTGTGAACAGTGCAACCACGGTTACAAAGGGCGCGTGGGGGT
CTACGAAGTGGTCAAGAACACGCCCGAACTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACCTGCAGA
TGCGCAAAGACGGTTTCAACGACCTACGCACCTCGGGGCTGCAAAAAGTGATGCAGGGCGTCACCAGCCTCGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.597

100

0.576
  pilB Acinetobacter baumannii D1279779

57.092

99.647

0.569
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

50.794

100

0.509
  pilB Vibrio campbellii strain DS40M4

48.94

100

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.104

98.587

0.484
  pilF Neisseria gonorrhoeae MS11

48.135

99.47

0.479
  pilF Thermus thermophilus HB27

36.042

100

0.36