Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMO40_RS06595 Genome accession   NZ_CP125805
Coordinates   1365220..1366611 (+) Length   463 a.a.
NCBI ID   WP_283385681.1    Uniprot ID   -
Organism   Mannheimia bovis strain 39324.S-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1360220..1371611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMO40_RS06565 (QMO40_06565) - 1360286..1361050 (-) 765 WP_283385678.1 TSUP family transporter -
  QMO40_RS06570 (QMO40_06570) proB 1361050..1362141 (-) 1092 WP_283385679.1 glutamate 5-kinase -
  QMO40_RS06575 (QMO40_06575) - 1362252..1362740 (+) 489 WP_283385680.1 dihydrofolate reductase -
  QMO40_RS06580 (QMO40_06580) - 1362866..1363096 (+) 231 WP_025236204.1 YgjV family protein -
  QMO40_RS06585 (QMO40_06585) radA 1363150..1364529 (-) 1380 WP_025236203.1 DNA repair protein RadA -
  QMO40_RS06590 (QMO40_06590) pilA 1364711..1365199 (+) 489 WP_025236202.1 prepilin peptidase-dependent pilin Machinery gene
  QMO40_RS06595 (QMO40_06595) pilB 1365220..1366611 (+) 1392 WP_283385681.1 GspE/PulE family protein Machinery gene
  QMO40_RS06600 (QMO40_06600) pilC 1366604..1367800 (+) 1197 WP_283385682.1 type II secretion system F family protein Machinery gene
  QMO40_RS06605 (QMO40_06605) - 1367804..1368460 (+) 657 WP_283385683.1 prepilin peptidase -
  QMO40_RS06610 (QMO40_06610) coaE 1368492..1369127 (+) 636 WP_283385684.1 dephospho-CoA kinase -
  QMO40_RS06615 (QMO40_06615) yacG 1369109..1369294 (+) 186 WP_283385685.1 DNA gyrase inhibitor YacG -
  QMO40_RS06620 (QMO40_06620) holA 1369295..1370317 (-) 1023 WP_283385686.1 DNA polymerase III subunit delta -
  QMO40_RS06625 (QMO40_06625) lptE 1370360..1370848 (-) 489 WP_283385687.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52317.02 Da        Isoelectric Point: 5.6752

>NTDB_id=832857 QMO40_RS06595 WP_283385681.1 1365220..1366611(+) (pilB) [Mannheimia bovis strain 39324.S-11]
MEYTVTDLANQEMIEISSEQWQKNCNEKQILLRYLAVPLQETQSQLWLAVDDMQNISACEIFAFIYHKQIEPVLISSDEL
KFLLNSLSPEQSSFQSEIYEDNVAYSLAHSEQIDRNDPIIQILDNLFKFCLKNNASDIHLEPQKEKLLIRLRIDGVLHIY
KTLPQSLSSRLISRIKLLAKLDISETRLPQDGQFHFKTILADTLDFRVSTLPTHLGEKVVLRLQKNKPTNLDFLELGFNQ
NQKKALLNALNQPQGLILVTGPTGSGKSITLYSALSHLNTSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEFELKNALLLVVAQRLLRRTCTKCGGI
GCENCYQGYKGRIGVYQLFSRSAKNFEKTTACLDFETLAESAKEKLAEGKTNQAEILRVLGNG

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=832857 QMO40_RS06595 WP_283385681.1 1365220..1366611(+) (pilB) [Mannheimia bovis strain 39324.S-11]
ATGGAGTATACTGTTACCGATTTAGCCAACCAAGAAATGATTGAAATTTCGAGCGAGCAGTGGCAGAAAAATTGCAATGA
AAAACAGATTTTATTGCGATATTTGGCTGTGCCACTGCAAGAAACACAATCTCAATTATGGTTGGCGGTAGACGATATGC
AAAATATCAGTGCTTGTGAGATTTTTGCCTTTATCTACCATAAACAGATTGAGCCTGTTTTGATTTCCTCCGATGAATTG
AAGTTTCTGCTCAATTCCCTTTCACCCGAACAGAGTAGCTTTCAGAGTGAAATTTATGAGGATAATGTAGCGTATTCATT
AGCACATTCGGAGCAAATAGACAGAAATGATCCGATTATCCAAATTTTAGATAATCTGTTTAAATTCTGTTTAAAAAATA
ATGCTTCAGATATTCATCTTGAACCGCAAAAAGAGAAATTACTCATTCGGCTGAGAATTGATGGCGTGCTGCATATTTAT
AAAACACTCCCACAAAGTTTATCGAGTAGACTGATTTCTCGCATTAAATTATTGGCTAAATTAGATATTAGTGAAACCCG
TTTACCACAAGATGGGCAATTTCACTTCAAAACAATTTTAGCGGATACCTTAGATTTCCGAGTTTCAACTCTACCTACCC
ATTTGGGTGAAAAAGTCGTCCTTCGGCTTCAAAAGAATAAACCGACTAATTTGGATTTTCTGGAGTTAGGTTTTAACCAA
AATCAAAAAAAAGCATTACTCAATGCACTCAACCAACCTCAAGGATTAATTTTAGTAACAGGTCCAACAGGTAGCGGAAA
AAGTATCACTTTATATAGTGCATTAAGCCATCTTAATACTAGCGAAAAGCATATATTAACGGCAGAAGATCCGATTGAAA
TTGAGATAGAAGGTATTATTCAAACCCAAGTAAACAGGGGCATTAATCTTGATTTCAGCCAACTGTTGAGAACCTTTCTC
CGTCAAGACCCCGACATCATTATGCTTGGGGAAATTCGTGATGAAGAGAGTGCGGAAATTGCCTTGAGAGCGGCTCAAAC
AGGGCATTTAGTGCTTTCAACATTGCATACCAATGATGCTCCTTCTGCAATTGAGCGATTGTTGCAACTCGGGATAAAAG
AATTCGAGCTGAAAAATGCGTTGTTGCTTGTAGTGGCTCAACGTTTATTAAGACGAACTTGCACAAAATGTGGCGGCATC
GGTTGCGAAAATTGCTATCAAGGCTACAAAGGAAGAATTGGGGTATATCAACTATTCAGCCGATCTGCAAAAAATTTTGA
AAAAACGACCGCTTGTTTGGATTTTGAAACCTTAGCTGAAAGTGCGAAAGAAAAATTAGCGGAAGGAAAGACCAATCAGG
CAGAAATTTTGAGGGTGTTAGGAAATGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(131-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

71.584

99.568

0.713
  pilB Haemophilus influenzae 86-028NP

57.709

98.056

0.566
  pilB Haemophilus influenzae Rd KW20

57.269

98.056

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

40.562

100

0.436
  pilB Vibrio cholerae strain A1552

41.494

100

0.432
  pilB Vibrio campbellii strain DS40M4

41.079

100

0.428
  pilB Legionella pneumophila strain ERS1305867

39.754

100

0.419
  pilB Acinetobacter baylyi ADP1

37.705

100

0.397
  pilB Acinetobacter baumannii D1279779

36.864

100

0.391