Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMK53_RS22460 Genome accession   NZ_CP125379
Coordinates   5055924..5057624 (-) Length   566 a.a.
NCBI ID   WP_320329062.1    Uniprot ID   -
Organism   Pseudomonas sp. P8_139     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5050924..5062624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK53_RS22425 (QMK53_22420) - 5051291..5051917 (-) 627 WP_003228301.1 DUF1780 domain-containing protein -
  QMK53_RS22430 (QMK53_22425) - 5052036..5052725 (+) 690 WP_320329063.1 energy-coupling factor ABC transporter permease -
  QMK53_RS22435 (QMK53_22430) - 5052791..5053006 (+) 216 WP_007909901.1 hypothetical protein -
  QMK53_RS22440 (QMK53_22435) yacG 5053013..5053213 (-) 201 WP_102356469.1 DNA gyrase inhibitor YacG -
  QMK53_RS22445 (QMK53_22440) coaE 5053210..5053833 (-) 624 WP_102356468.1 dephospho-CoA kinase -
  QMK53_RS22450 (QMK53_22445) pilD 5053830..5054702 (-) 873 WP_102356467.1 prepilin peptidase Machinery gene
  QMK53_RS22455 (QMK53_22450) pilC 5054704..5055921 (-) 1218 WP_102356466.1 type II secretion system F family protein Machinery gene
  QMK53_RS22460 (QMK53_22455) pilB 5055924..5057624 (-) 1701 WP_320329062.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK53_RS22465 (QMK53_22460) pilA 5057850..5058290 (+) 441 WP_102356464.1 pilin Machinery gene
  QMK53_RS22470 (QMK53_22465) - 5058583..5058939 (+) 357 WP_320329061.1 BON domain-containing protein -
  QMK53_RS22475 (QMK53_22470) - 5059211..5059507 (-) 297 WP_102356462.1 DUF2845 domain-containing protein -
  QMK53_RS22480 (QMK53_22475) - 5059507..5062455 (-) 2949 WP_320329060.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62610.50 Da        Isoelectric Point: 5.8283

>NTDB_id=831660 QMK53_RS22460 WP_320329062.1 5055924..5057624(-) (pilB) [Pseudomonas sp. P8_139]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVRSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTHASGLEEMADVDLDG
VDVESVDDSRQENIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFSQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPP
EQISSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDSQMRHDGFDDLRTSGLRKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=831660 QMK53_RS22460 WP_320329062.1 5055924..5057624(-) (pilB) [Pseudomonas sp. P8_139]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAACTGGTACAGGCTGAACTGTTGACGGAAAAAAGCGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAGGAGTTCCCAGGTCG
CCGAAATCGCTTCCGAGCATTTCGGCATGGCCTTCATGGACCTCAATTGCCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGTCAGCACCACGCCCTGCCCTTGTGGCGACGCGGCAACAAGCTGTTCGTGGGCATTTCCGA
TCCGAGCAATCATCAGGCAATCAACGATATCCAGTTCAGCACCGGACTGAGTACCGAAGCCATCCTGGTGGAAGACGACA
AACTCAGCGATGCCATCGAGAAATTTTTCGACACCCACGCCAGCGGCCTCGAAGAGATGGCCGACGTCGACCTCGACGGG
GTGGACGTCGAATCCGTCGACGACAGCCGACAGGAGAATATCGGCGGACTCGATGCCGATGACGCCCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGTGGCTCGTCGGACTTGCACTTCGAACCCTACGAAAAGAACTTCCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAAGTGGCCAAACCGCCGATTCAGTTGGCCGGGCGGATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGACATCTCGGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGAGCAA
GTCGATCGACTTCCGGGTCAACACCCTGCCCACCCTGTGGGGCGAGAAAGTGGTGATCCGGATTCTCGATCCGTCCAGCG
CGCAGATCGGCATCGATGCCCTGGGTTACGAACCGGCGCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCGCAGGGG
ATGATTCTGGTGACCGGCCCGACCGGGTCGGGGAAAACCGTGTCGCTGTACACCGGACTCAATATTCTCAATACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGATTTCTCCCAGGCATTACGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAAATTCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACCTCGTGTTGTCCACGCTGCACACCAACAGCGCCGCCGA
AACCCTGACCCGCCTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGCC
TGGCACGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATTCCGCGCGAAACGCTGATCAAGGAAGGCTTCCCACCG
GAACAGATCAGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGATCAATGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCCGAGCTGCAACGGCTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACAGCCAGA
TGCGTCACGACGGTTTCGACGATCTGCGCACCTCAGGCCTGCGCAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

55.752

99.823

0.557
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio campbellii strain DS40M4

50

99.647

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

93.463

0.362
  pilF Thermus thermophilus HB27

36.738

98.587

0.362