Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QMK53_RS22450 Genome accession   NZ_CP125379
Coordinates   5053830..5054702 (-) Length   290 a.a.
NCBI ID   WP_102356467.1    Uniprot ID   A0A2N7R9Z2
Organism   Pseudomonas sp. P8_139     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5048830..5059702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK53_RS22410 (QMK53_22405) - 5049180..5050478 (-) 1299 WP_320329065.1 NAD(P)/FAD-dependent oxidoreductase -
  QMK53_RS22415 (QMK53_22410) - 5050542..5050715 (-) 174 WP_102356472.1 DUF3094 domain-containing protein -
  QMK53_RS22420 (QMK53_22415) - 5050767..5051294 (-) 528 WP_320329064.1 MOSC domain-containing protein -
  QMK53_RS22425 (QMK53_22420) - 5051291..5051917 (-) 627 WP_003228301.1 DUF1780 domain-containing protein -
  QMK53_RS22430 (QMK53_22425) - 5052036..5052725 (+) 690 WP_320329063.1 energy-coupling factor ABC transporter permease -
  QMK53_RS22435 (QMK53_22430) - 5052791..5053006 (+) 216 WP_007909901.1 hypothetical protein -
  QMK53_RS22440 (QMK53_22435) yacG 5053013..5053213 (-) 201 WP_102356469.1 DNA gyrase inhibitor YacG -
  QMK53_RS22445 (QMK53_22440) coaE 5053210..5053833 (-) 624 WP_102356468.1 dephospho-CoA kinase -
  QMK53_RS22450 (QMK53_22445) pilD 5053830..5054702 (-) 873 WP_102356467.1 prepilin peptidase Machinery gene
  QMK53_RS22455 (QMK53_22450) pilC 5054704..5055921 (-) 1218 WP_102356466.1 type II secretion system F family protein Machinery gene
  QMK53_RS22460 (QMK53_22455) pilB 5055924..5057624 (-) 1701 WP_320329062.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK53_RS22465 (QMK53_22460) pilA 5057850..5058290 (+) 441 WP_102356464.1 pilin Machinery gene
  QMK53_RS22470 (QMK53_22465) - 5058583..5058939 (+) 357 WP_320329061.1 BON domain-containing protein -
  QMK53_RS22475 (QMK53_22470) - 5059211..5059507 (-) 297 WP_102356462.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32169.40 Da        Isoelectric Point: 7.7104

>NTDB_id=831658 QMK53_RS22450 WP_102356467.1 5053830..5054702(-) (pilD) [Pseudomonas sp. P8_139]
MPIDELFSLYPLAFVFTALLLGLVVGSFLNVLVWRLPKMLERDWRQQAQDVLGLPSDTPLPTYNLILPHSECPQCGHRIR
VWENIPLLSYLFLRGRCSACAAPISKRYPLTELACGLLSAFIAWHLGFGWPAGLLIVLTWGLLAMSLIDTEHQLLPDVLV
LPLLWLGLIVNSFAVFVSLHAALWGAVAGYMALWSVFWLFKLLTGKDGIGHGDFKLLALLGAWGGWQILPLTILLSSLVG
AVVGVIVLRLREQKTSTPIPFGPYLAIAGWIALLWGGQITDFYWQFVGLK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=831658 QMK53_RS22450 WP_102356467.1 5053830..5054702(-) (pilD) [Pseudomonas sp. P8_139]
ATGCCTATCGACGAACTTTTCAGTCTGTATCCGCTGGCCTTCGTTTTCACTGCACTGCTGCTGGGTCTGGTGGTCGGCAG
TTTTCTCAACGTGCTGGTCTGGCGTCTGCCGAAAATGCTCGAGCGCGATTGGCGCCAGCAGGCTCAGGACGTCCTCGGCC
TGCCGAGTGATACGCCACTGCCGACTTACAACCTGATACTGCCGCACTCCGAATGCCCGCAATGCGGCCACCGAATTCGT
GTATGGGAGAACATCCCGCTGCTCAGCTACCTGTTCCTGCGCGGACGCTGTTCTGCGTGTGCCGCCCCGATCAGCAAGCG
CTATCCCCTGACTGAACTGGCCTGCGGTCTGCTCTCGGCGTTCATCGCCTGGCATCTGGGTTTTGGCTGGCCGGCCGGTC
TGTTGATCGTCCTCACCTGGGGGCTGCTGGCGATGAGCCTGATCGATACCGAACATCAACTGCTGCCCGATGTGCTGGTG
CTGCCGTTGCTGTGGCTGGGGTTGATCGTCAACAGCTTCGCAGTGTTCGTCTCGCTGCACGCGGCACTATGGGGCGCGGT
GGCCGGTTACATGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGCTGACCGGCAAAGACGGCATCGGTCATGGTGATT
TCAAGCTGCTGGCGCTGCTCGGGGCCTGGGGTGGCTGGCAGATTCTGCCGCTGACGATCCTGTTGTCATCGCTGGTGGGG
GCCGTTGTCGGGGTGATTGTGCTGCGTCTGCGCGAACAGAAAACCTCGACGCCGATCCCCTTCGGCCCGTATCTGGCAAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTTCTATTGGCAGTTTGTCGGTTTGAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(136-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7R9Z2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.546

97.241

0.521
  pilD Vibrio campbellii strain DS40M4

51.773

97.241

0.503
  pilD Acinetobacter baumannii D1279779

47.985

94.138

0.452
  pilD Acinetobacter nosocomialis M2

47.985

94.138

0.452
  pilD Neisseria gonorrhoeae MS11

47.778

93.103

0.445