NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	QMK53_RS22465	Genome accession	NZ_CP125379
Coordinates	5057850..5058290 (+)	Length	146 a.a.
NCBI ID	WP_102356464.1	Uniprot ID	A0A2N7RA05
Organism	Pseudomonas sp. P8_139
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5052850..5063290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMK53_RS22440 (QMK53_22435)	yacG	5053013..5053213 (-)	201	WP_102356469.1	DNA gyrase inhibitor YacG	-
QMK53_RS22445 (QMK53_22440)	coaE	5053210..5053833 (-)	624	WP_102356468.1	dephospho-CoA kinase	-
QMK53_RS22450 (QMK53_22445)	pilD	5053830..5054702 (-)	873	WP_102356467.1	prepilin peptidase	Machinery gene
QMK53_RS22455 (QMK53_22450)	pilC	5054704..5055921 (-)	1218	WP_102356466.1	type II secretion system F family protein	Machinery gene
QMK53_RS22460 (QMK53_22455)	pilB	5055924..5057624 (-)	1701	WP_320329062.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QMK53_RS22465 (QMK53_22460)	pilA	5057850..5058290 (+)	441	WP_102356464.1	pilin	Machinery gene
QMK53_RS22470 (QMK53_22465)	-	5058583..5058939 (+)	357	WP_320329061.1	BON domain-containing protein	-
QMK53_RS22475 (QMK53_22470)	-	5059211..5059507 (-)	297	WP_102356462.1	DUF2845 domain-containing protein	-
QMK53_RS22480 (QMK53_22475)	-	5059507..5062455 (-)	2949	WP_320329060.1	DUF748 domain-containing protein	-

Sequence

Protein

Download Length: 146 a.a. Molecular weight: 14574.71 Da Isoelectric Point: 8.1087

                                >NTDB_id=831661 QMK53_RS22465 WP_102356464.1 5057850..5058290(+) (pilA) [Pseudomonas sp. P8_139]

MKQQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLLLVAGSATATTSNCT

LAASGTASTGAGTITCTLVNAPGPVLGKTITLTRTGAATGNTSGVWSCASTVNPDYAPKGCAGTGA

Nucleotide

Download Length: 441 bp

                                >NTDB_id=831661 QMK53_RS22465 WP_102356464.1 5057850..5058290(+) (pilA) [Pseudomonas sp. P8_139]

ATGAAACAACAACAGGGCTTTACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATTGGCATTCTGGCGACTATCGCCTT

GCCGCAATACTCCAAATACCAGGCACGTTCGAAAGTGACTGCGGGTCTGGCGGAGATCAGTGCACTGAAGGTGCCGTTCG

AGGACACCATCAATCAAGGCACTGATCCAACGCTATTACTGGTGGCCGGTAGCGCTACAGCGACCACCTCCAACTGCACG

CTGGCGGCGTCGGGAACCGCCTCGACCGGTGCCGGCACTATCACCTGCACACTGGTGAATGCTCCAGGTCCGGTGCTCGG

TAAAACCATTACGTTGACCCGTACCGGTGCTGCAACCGGCAACACCTCCGGCGTCTGGAGCTGTGCGTCGACCGTCAACC

CGGACTACGCGCCTAAGGGTTGCGCAGGCACCGGTGCCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A2N7RA05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Acinetobacter baumannii strain A118	48.299	100	0.486
pilA	Pseudomonas aeruginosa PAK	42.208	100	0.445
pilA	Vibrio cholerae strain A1552	42.667	100	0.438
pilA	Vibrio cholerae C6706	42.667	100	0.438
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.667	100	0.438
pilA/pilAII	Pseudomonas stutzeri DSM 10701	44.681	96.575	0.432
pilA/pilAI	Pseudomonas stutzeri DSM 10701	44.366	97.26	0.432
comP	Acinetobacter baylyi ADP1	39.103	100	0.418
pilA	Ralstonia pseudosolanacearum GMI1000	37.195	100	0.418
pilA	Vibrio parahaemolyticus RIMD 2210633	41.135	96.575	0.397
pilA2	Legionella pneumophila str. Paris	40.141	97.26	0.39
pilA2	Legionella pneumophila strain ERS1305867	40.141	97.26	0.39

Detailed information NTG0831661