Detailed information    

experimental Experimentally validated

Overview

Name   recQ   Type   Machinery gene
Locus tag   BSU_19220 Genome accession   NC_000964
Coordinates   2094010..2095785 (-) Length   591 a.a.
NCBI ID   NP_389803.1    Uniprot ID   O34748
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

RecQ[Bs] binds weakly to single-stranded DNA (ssDNA) and blunt-ended double-stranded DNA (dsDNA) but strongly to forked dsDNA. The protein exhibits a DNA-stimulated ATPase activity and ATP- and Mg(2+)-dependent DNA helicase activity with a 3' → 5' polarity.


Genomic Context

Location: 2089010..2100785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_19180 (BSU19180) desE 2089396..2090454 (+) 1059 NP_389799.1 fatty acid desaturase -
  BSU_19190 (BSU19190) desK 2090574..2091686 (+) 1113 NP_389800.1 two-component thermosensor histidine kinase [DesR-DesK] -
  BSU_19200 (BSU19200) desR 2091705..2092304 (+) 600 NP_389801.1 two-component response regulator [DesK] -
  BSU_19210 (BSU19210) walL 2092899..2093762 (-) 864 NP_389802.1 exported cell wall lytic enzyme -
  BSU_19220 (BSU19220) recQ 2094010..2095785 (-) 1776 NP_389803.1 ATP-dependent helicase Machinery gene
  BSU_19230 (BSU19230) azoJ 2096350..2096976 (-) 627 NP_389804.1 FMN-dependent NADH-azoreductase -
  BSU_19240 (BSU19240) yocK 2097126..2097617 (-) 492 NP_389805.2 putative general stress protein -
  BSU_19250 (BSU19250) yocL 2097692..2098024 (-) 333 NP_389806.1 hypothetical protein -
  BSU_19259 (BSU19259) yoyB 2098102..2098329 (+) 228 YP_003097740.1 hypothetical protein -
  BSU_19260 (BSU19260) yocM 2098316..2098792 (-) 477 NP_389808.1 putative chaperone -
  BSU_19270 (BSU19270) yozN 2098859..2099122 (+) 264 NP_389809.1 conserved protein of unknown function (mother cell in sporulation) -
  BSU_19280 (BSU19280) yocN 2099127..2099360 (+) 234 NP_389810.1 conserved protein of unknown function (sporulation-related) -
  BSU_19290 (BSU19290) yozO 2099446..2099790 (-) 345 NP_389811.1 hypothetical protein -
  BSU_19300 (BSU19300) yozC 2100147..2100350 (-) 204 NP_389812.1 hypothetical protein -

Sequence

Protein


Download         Length: 591 a.a.        Molecular weight: 67352.87 Da        Isoelectric Point: 6.2902

>NTDB_id=638 BSU_19220 NP_389803.1 2094010..2095785(-) (recQ) [Bacillus subtilis subsp. subtilis str. 168]
MLHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGTTIVISPLISLMKDQVDALEE
AGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLTSIEFIRILQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEI
LFRELHDKPVIMALTATATPEVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR
KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIRFVLHAQIPKDMESYYQEAGR
AGRDGLASECVLLFSPQDIMVQRFLIEQSEHEEKQKQDLKKLRQMVDYCHTEDCLQRFILMYFGEKEPDACGQCGNCTDT
RAAHDVTREAQMVLSCIIRMKERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRMSD
GTFPTLFVSSKGRNVLKGELSVARKEALKAAAITENDELFERLRMVRKEIAAEQGVPPFVVFSDQTLKEMSGKQPVNDDE
LLSIKGVGEQKRAKYGRLFLQEIQAYARMTD

Nucleotide


Download         Length: 1776 bp        

>NTDB_id=638 BSU_19220 NP_389803.1 2094010..2095785(-) (recQ) [Bacillus subtilis subsp. subtilis str. 168]
ATGTTACATAGAGCCCAATCCCTTCTGGCTCATTATTTTGGTTATGAGAAGTTTCGGAGCGGCCAAGATGAAGCGATCCG
CTTGGTGACGGAAGCGAGGCAAAATACGGCCTGCATTATGCCGACGGGAGGCGGCAAATCGATTTGCTATCAGATTCCGG
CATTAATGTTTGAAGGGACGACGATCGTCATTTCACCGTTGATTTCCTTGATGAAGGATCAAGTTGATGCGTTAGAAGAA
GCGGGGATTAACGCCGCCTACATTAACAGCACACAATCCAATCAGGAAATATATGAGAGGCTGAACGGACTCAAAGAAGG
GGCTTATAAGCTTTTTTACATTACACCAGAGCGACTAACGTCCATAGAGTTTATCCGGATTTTGCAGGGCATAGATGTTC
CTTTGGTCGCAATAGACGAGGCGCATTGTATTTCTCAATGGGGCCATGATTTCAGGCCAAGCTACAGGAATATTGAAATC
TTGTTCCGTGAATTACACGACAAGCCTGTCATCATGGCGTTAACAGCAACAGCCACACCCGAGGTTCATGATGATATTTG
CAAACAGCTGCATATTCAAAAAGAAAACACGGTATATACCGGATTTTCCCGAGAGAATTTAACCTTTAAAGTAGTAAAAG
GAGAAAATAAAGATCGGTTTATAGACGAATATGTGCAAAACAACAGGCATGAAGCAGGAATTGTCTATACAGCGACCAGA
AAAGAAGCTGATCGGATATATGAGAGATTGAAACGAAATCAGGTCAGGGCAGGCCGGTATCATGGAGGTTTGGCTGATGA
TGTGAGAAAAGAGCAGCAGGAAAGATTTCTTAATGATGAGCTTCAAGTGATGGTGGCAACCTCTGCTTTTGGAATGGGTA
TTGATAAATCAAATATCAGATTTGTTCTGCATGCACAAATCCCGAAAGATATGGAAAGCTATTATCAAGAGGCAGGACGA
GCTGGACGGGACGGTCTTGCCAGTGAATGTGTTCTCCTGTTTTCACCGCAGGATATTATGGTGCAGCGCTTCTTAATTGA
GCAATCAGAACATGAAGAGAAACAAAAGCAGGATTTAAAAAAGCTGAGACAAATGGTCGACTACTGTCATACGGAAGATT
GTCTGCAGCGTTTTATCCTTATGTATTTCGGAGAAAAGGAGCCTGATGCGTGCGGACAGTGCGGAAACTGTACCGATACA
AGAGCGGCCCATGACGTGACGCGGGAGGCGCAAATGGTCTTATCTTGTATCATTCGCATGAAAGAACGGTTTGGCAAGAC
GATGGTTGCCCAGGTGCTTGCCGGCTCCAAAAATAAAAAAGTTCTTGAAAACGGCTTTTCCGACCTTTCAACATACGGGA
TATTAAAACACCAATCCGTTGGCGAAATCAGTGATTTTATCGAATTTCTTATTTCTGACGACTTCATCAGGATGTCAGAC
GGAACGTTTCCGACTCTTTTTGTCAGCAGTAAAGGAAGGAATGTGCTGAAAGGAGAATTATCAGTAGCGAGAAAAGAAGC
GTTAAAAGCGGCAGCGATTACTGAAAATGACGAGCTATTTGAACGATTGCGCATGGTTCGGAAAGAAATTGCGGCTGAGC
AGGGTGTTCCGCCATTTGTTGTTTTCTCTGACCAAACGTTAAAAGAAATGTCAGGAAAACAGCCCGTAAATGATGATGAA
CTTTTGTCTATAAAAGGAGTCGGAGAACAAAAAAGAGCAAAATACGGCCGGCTTTTTTTACAAGAAATACAGGCTTATGC
AAGGATGACAGACTAA

Domains

Predicted by InterProScan.

RQC domain (RQC)

(403-507)

RecQ zinc-binding (RecQ_Zn_bind)

(336-398)

DEAD/DEAH box helicase (DEAD)

(21-182)

Helicase conserved C-terminal domain (Helicase_C)

(218-324)

HRDC domain (HRDC)

(518-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34748

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Wei Qin et al. (2014) Characterization of biochemical properties of Bacillus subtilis RecQ helicase. Journal of Bacteriology 196(24):4216-28. [PMID: 25246477]