Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QMK55_RS15585 Genome accession   NZ_CP125378
Coordinates   3431557..3433629 (+) Length   690 a.a.
NCBI ID   WP_102355614.1    Uniprot ID   A0A2N7RDV7
Organism   Pseudomonas sp. P8_229     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3426557..3438629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK55_RS15565 (QMK55_15560) pilM 3428769..3429833 (+) 1065 WP_320329512.1 pilus assembly protein PilM Machinery gene
  QMK55_RS15570 (QMK55_15565) - 3429833..3430399 (+) 567 WP_320329513.1 PilN domain-containing protein -
  QMK55_RS15575 (QMK55_15570) pilO 3430396..3431019 (+) 624 WP_102355616.1 type 4a pilus biogenesis protein PilO -
  QMK55_RS15580 (QMK55_15575) - 3431016..3431543 (+) 528 WP_320329514.1 pilus assembly protein PilP -
  QMK55_RS15585 (QMK55_15580) pilQ 3431557..3433629 (+) 2073 WP_102355614.1 type IV pilus secretin PilQ Machinery gene
  QMK55_RS15590 (QMK55_15585) aroK 3433634..3434152 (+) 519 WP_024011270.1 shikimate kinase AroK -
  QMK55_RS15595 (QMK55_15590) aroB 3434304..3435404 (+) 1101 WP_102355613.1 3-dehydroquinate synthase -
  QMK55_RS15600 (QMK55_15595) - 3435415..3437001 (+) 1587 WP_102355612.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74867.26 Da        Isoelectric Point: 5.5748

>NTDB_id=831629 QMK55_RS15585 WP_102355614.1 3431557..3433629(+) (pilQ) [Pseudomonas sp. P8_229]
MNRIFSTLGFSLWIALLSPMVLAASLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLTSKTRD
LGSGNARSATVVEANDRTRLIINLTQLSPYTFRVEGNNLFVVVGQGAKPAAPRPAAVAPRPAAAPAKVYAPVAKAIRGVD
FQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILSFARTQLPDRLRVRLDVKDFATPVQFVNSAATGDRAIITIEPSGTFD
YSTYQTDNKLTVSIRPMTVDDLQKRNAERQAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKID
ERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNGS
STTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=831629 QMK55_RS15585 WP_102355614.1 3431557..3433629(+) (pilQ) [Pseudomonas sp. P8_229]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCACCGATGGTTCTGGCGGCCAGCCTGAA
GACGCTGGATGTGGCGGCATTGCCCGGCGACCGCGTCGAGCTGAAGCTGTCCTTCGACGGCCCGCCCCCACAGCCCAAGG
GCTACACCACCGAGTCGCCGGCACGAATCGCGCTGGATCTGCCGGGTGTCGCCAGTCAGCTGACGAGCAAGACCCGCGAT
CTGGGCAGCGGCAACGCACGCAGCGCCACGGTGGTCGAGGCCAATGATCGCACGCGATTGATCATCAACCTGACGCAGCT
TAGCCCTTACACTTTCCGGGTCGAGGGCAACAATCTGTTCGTGGTGGTCGGGCAGGGCGCCAAGCCTGCTGCACCACGTC
CGGCCGCTGTTGCGCCACGGCCAGCCGCAGCTCCGGCGAAGGTTTACGCGCCTGTCGCCAAAGCCATTCGTGGCGTCGAC
TTCCAGCGCGGCACCGCCGGGGAGGGCAACGTAGTTATCGACTTGTCGGACCCTGCCATCGCCCCGGACATCCAGGAGCA
TGACGGCAAGATCATCCTCAGCTTTGCCCGCACCCAGTTGCCCGACCGACTGCGGGTGCGCCTCGACGTCAAGGATTTCG
CCACCCCGGTGCAGTTCGTCAACAGCGCCGCGACCGGCGATCGCGCGATCATTACCATCGAGCCCAGCGGCACTTTCGAC
TATTCGACCTACCAGACCGACAACAAGCTGACTGTCAGTATTCGCCCGATGACGGTCGATGACCTGCAAAAGCGCAATGC
TGAACGCCAGGCCTACAGTGGCGAAAAACTCTCGCTGAATTTCCAGGACATCGACGTACGCTCGGTGCTGCAACTGATCG
CCGACTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGCCTGCAAAACGTACCGTGG
GATCAGGCTCTGGACCTGGTGCTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAACGTGTTGCTGGTGGCGCCGGC
TGACGAAATCGCTGCCCGTGAGCGTCAGGAACTGGAGTCGCAGAAACAGATCGCCGAACTGGCTCCGCTGCGCCGCGAGC
TGCTGCAAGTGAACTACGCCAAGGCGGCGGACATCGCCAAACTGTTCCAGTCGGTGACCAGCGCCGAAGCGAAAATCGAC
GAGCGCGGCTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGAGCTGCG
ACGGATCGTGGCGCAGCTGGATATTCCAGTGCGCCAGGTGATGATCGAGGCGCGAATCGTCGAAGCCAACGTCGATTACG
ACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAAGGCAACTGGAACACGTCCGGGGTCAGTAACGGCTCC
TCGACAACCATTGGCACGCCGGGCAGCACCAGCACCAACTCGCCATTTGTCGACATGGGCACGGTCGGTAATACCTCTGG
GATCGGCATCGCCTTCATCACCGACAACGTGCTGCTCGATCTTGAGCTGACGGCGATGGAGAAAACCGGCAACGGCGAAA
TCGTCTCCCAACCCAAAGTGGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTATCAGGAA
GCCAGTTCCAGCGGCGCGACTTCGGTGTCATTCAAGGAGGCGTCGCTGTCGCTGGAAGTGACGCCGCAGATCACCCCCGA
CAACCGCATTATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTGCAGGATGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAAGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGCGGTGTTTTCTCAAATACTCAA
AGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Secretin and TonB N terminus short domain (STN)

(300-346)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7RDV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.566

100

0.762
  comQ Acinetobacter baylyi ADP1

41.534

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.057

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.057

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

38.599

97.246

0.375
  pilQ Legionella pneumophila strain Lp02

38.599

97.246

0.375