Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QMK55_RS15565 Genome accession   NZ_CP125378
Coordinates   3428769..3429833 (+) Length   354 a.a.
NCBI ID   WP_320329512.1    Uniprot ID   -
Organism   Pseudomonas sp. P8_229     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3423769..3434833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK55_RS15555 (QMK55_15550) - 3424570..3425838 (+) 1269 WP_102355620.1 malic enzyme-like NAD(P)-binding protein -
  QMK55_RS15560 (QMK55_15555) - 3426105..3428549 (-) 2445 WP_371859052.1 penicillin-binding protein 1A -
  QMK55_RS15565 (QMK55_15560) pilM 3428769..3429833 (+) 1065 WP_320329512.1 pilus assembly protein PilM Machinery gene
  QMK55_RS15570 (QMK55_15565) - 3429833..3430399 (+) 567 WP_320329513.1 PilN domain-containing protein -
  QMK55_RS15575 (QMK55_15570) pilO 3430396..3431019 (+) 624 WP_102355616.1 type 4a pilus biogenesis protein PilO -
  QMK55_RS15580 (QMK55_15575) - 3431016..3431543 (+) 528 WP_320329514.1 pilus assembly protein PilP -
  QMK55_RS15585 (QMK55_15580) pilQ 3431557..3433629 (+) 2073 WP_102355614.1 type IV pilus secretin PilQ Machinery gene
  QMK55_RS15590 (QMK55_15585) aroK 3433634..3434152 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38204.78 Da        Isoelectric Point: 4.8983

>NTDB_id=831628 QMK55_RS15565 WP_320329512.1 3428769..3429833(+) (pilM) [Pseudomonas sp. P8_229]
MLGLFNKKTNMLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPPNAVVEKNIAELEGVGHTLSRVLVKARTGLRSVAV
AVAGSAVITKVIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=831628 QMK55_RS15565 WP_320329512.1 3428769..3429833(+) (pilM) [Pseudomonas sp. P8_229]
GTGCTGGGACTCTTCAATAAAAAGACCAATATGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGTTGGAGCT
GAGCCGCCAGGGCGAGCGCTATCGGGTCGAGGCCTATGCGGTGGAACCGCTGCCGCCCAATGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAAGGTGTGGGTCACACCTTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAGGAGCGTGGCGGTG
GCAGTGGCCGGTTCTGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGACCGACGACGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGACCAGTACATTCCCTACCCGCTGGACGAAGTCGCCATCGATTTCGAAGTTCAGGGTGTGTCGCCGC
GCAACCCGGAACGGGTCAACGTGTTGCTCGCCGCCTGCCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCGCTG
GCGGGGCTGACTGCGCGGGTGGTCGATGTCGAGGCCTACGCACTGGAGCGCTCATTCGGTCTGCTAGCCACCCAGTTGGC
GGCCTCGCAGGAGCGCCTGACGGTCGCGGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACAACGGCC
GCATCATCTATACCCGCGAGCAACTGTTCGGTGGTCGGCAACTGACCGAAGAGATCCAGCGTCGCTATGGCCTGACCGTC
GAGCAGGCCGGACTGGCGAAAAAGCAGGGTGGCCTGCCCGACGACTATGTCAGCGAAGTCTTGCAGCCGTTCCGTGATGC
GCTGGTGCAGCAGGTGTCGCGTTCATTGCAGTTTTTCTTTGCCTCCGGGCAGTACAACGCCGTCGATCACATCCTTTTGG
CCGGTGGCACGGCGTCGGTGCCAGGGTTGGATCGCCTGATCGAGCAACGGCTGGGCACTCCGACTCAAGTCGCCAATCCG
TTTTCCAATATGGCCTTGAGCAGCAAGGTCAACGCGGGGGCCCTGGCCAGTGATGCGCCGGCCTTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486