Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QMK48_RS02295 Genome accession   NZ_CP125374
Coordinates   512784..514874 (+) Length   696 a.a.
NCBI ID   WP_253471099.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507784..519874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK48_RS02275 (QMK48_02275) pilM 509996..511060 (+) 1065 WP_214510899.1 pilus assembly protein PilM Machinery gene
  QMK48_RS02280 (QMK48_02280) - 511060..511626 (+) 567 WP_258690225.1 PilN domain-containing protein -
  QMK48_RS02285 (QMK48_02285) pilO 511623..512246 (+) 624 WP_214510897.1 type 4a pilus biogenesis protein PilO -
  QMK48_RS02290 (QMK48_02290) - 512243..512770 (+) 528 WP_253471097.1 pilus assembly protein PilP -
  QMK48_RS02295 (QMK48_02295) pilQ 512784..514874 (+) 2091 WP_253471099.1 type IV pilus secretin PilQ Machinery gene
  QMK48_RS02300 (QMK48_02300) aroK 514879..515397 (+) 519 WP_024616288.1 shikimate kinase AroK -
  QMK48_RS02305 (QMK48_02305) aroB 515546..516646 (+) 1101 WP_320389794.1 3-dehydroquinate synthase -
  QMK48_RS02310 (QMK48_02310) - 516656..518236 (+) 1581 WP_253471102.1 AAA family ATPase -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75231.41 Da        Isoelectric Point: 5.4242

>NTDB_id=831499 QMK48_RS02295 WP_253471099.1 512784..514874(+) (pilQ) [Pseudomonas sp. P9_32]
MNRFFSALGISLWIALLSPMVLGASLKTLDVAALPGDRVELKLSFDGPPPPPRGYTTEQPARIALDLPGVTNQLSSKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGVAPKTVAPKNSTTQPPRPAVASAAPARASAPAGK
AIRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIE
PSGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKADERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNQGNWNSS
GVTNGTSTTIGTPGSTGTNQPFVDMGTSGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=831499 QMK48_RS02295 WP_253471099.1 512784..514874(+) (pilQ) [Pseudomonas sp. P9_32]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCTCGGGGCAAGCCTGAA
GACGTTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCTCCTTCGACGGGCCGCCGCCGCCGCCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCGCTGGACCTGCCGGGCGTGACCAATCAACTTTCCAGCAAGCGCCGCGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACGGTGGTCGAGGCTGGGGATCGTACTCGGTTGATCATCGGCCTTACCCAGCT
GGCGCCGTATGACACCCGGATCGAAGGTAACAACCTGTTCGTGGTGGTTGGCAAGGGCGTCGCCCCGAAGACCGTCGCCC
CGAAGAACTCCACCACCCAGCCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCTGCGAGGGCCAGTGCGCCGGCGGGCAAG
GCGATCCGAGGAGTGGATTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTCGTGATCGACCTGTCTGATCCGTCCATCGC
CCCGGATATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCTGAGCCGCTGCGCGTGCGCC
TGGACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAA
CCCAGCGGGACCTTCGACTACTCGACCTACCAGACCGATAACAAGCTCACTGTCAGCGTCCGGCCCATGACCGTCGACGA
CCTGCAAAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGTT
CGGTCCTGCAGCTGATTGCCGATTTTACCAACCTGAACCTCGTGGCCAGCGACACGGTGCAGGGTGGCATCACCTTGCGG
TTGCAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAATGT
GCTGTTGGTGGCTCCGGCCGATGAAATTGCCGCGCGTGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGACCTGG
CGCCGCTGCGGCGTGAGCTGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGTTGTTCCAGTCGGTGACCAGT
GCCGAGGCCAAAGCCGATGAGCGCGGCTCGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGA
CCGCCTCGACGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAAGCGCGGATTGTCG
AAGCCAACGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGTGGTTCGGTACAGAACCAGGGCAACTGGAATTCCTCC
GGGGTCACCAACGGCACTTCCACCACCATCGGCACCCCGGGCAGTACCGGCACCAACCAACCGTTCGTCGACATGGGCAC
CTCGGGCAACACCTCGGGCATCGGCATCGCGTTCATCACCGACAACGTATTGTTGGACCTTGAGCTGACGGCCATGGAGA
AAACCGGCAACGGCGAAATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACG
GAAATTCCTTATCAGGAAGCCAGCTCCAGTGGCGCCACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGAC
CCCGCAGATCACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGA
ATGATGTGCCGCCGATCAAGAAAAACGAAGTCAATGCCAAGGTCCTGGTCAATGACGGCGAGACCATTGTGATTGGTGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Secretin and TonB N terminus short domain (STN)

(306-352)

AMIN domain (AMIN)

(37-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.007

100

0.75
  comQ Acinetobacter baylyi ADP1

41.463

100

0.415
  pilQ Acinetobacter baumannii strain A118

38.184

100

0.411
  pilQ Acinetobacter baumannii D1279779

38.184

100

0.411
  pilQ Legionella pneumophila strain Lp02

38.109

97.27

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.962

97.27

0.369