Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QMK48_RS02275 Genome accession   NZ_CP125374
Coordinates   509996..511060 (+) Length   354 a.a.
NCBI ID   WP_214510899.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504996..516060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK48_RS02265 (QMK48_02265) - 505529..506797 (+) 1269 WP_258690224.1 malic enzyme-like NAD(P)-binding protein -
  QMK48_RS02270 (QMK48_02270) - 507344..509788 (-) 2445 WP_413817777.1 penicillin-binding protein 1A -
  QMK48_RS02275 (QMK48_02275) pilM 509996..511060 (+) 1065 WP_214510899.1 pilus assembly protein PilM Machinery gene
  QMK48_RS02280 (QMK48_02280) - 511060..511626 (+) 567 WP_258690225.1 PilN domain-containing protein -
  QMK48_RS02285 (QMK48_02285) pilO 511623..512246 (+) 624 WP_214510897.1 type 4a pilus biogenesis protein PilO -
  QMK48_RS02290 (QMK48_02290) - 512243..512770 (+) 528 WP_253471097.1 pilus assembly protein PilP -
  QMK48_RS02295 (QMK48_02295) pilQ 512784..514874 (+) 2091 WP_253471099.1 type IV pilus secretin PilQ Machinery gene
  QMK48_RS02300 (QMK48_02300) aroK 514879..515397 (+) 519 WP_024616288.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38065.62 Da        Isoelectric Point: 4.9070

>NTDB_id=831498 QMK48_RS02275 WP_214510899.1 509996..511060(+) (pilM) [Pseudomonas sp. P9_32]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKARTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVIGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGSASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALAGDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=831498 QMK48_RS02275 WP_214510899.1 509996..511060(+) (pilM) [Pseudomonas sp. P9_32]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTGGAGTT
GAGCCGCCAGGGTGATCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGGCAGGCGTTGTCGCGCCTGTTGGCCAAGGCCAGGACGCCCTTGCGTAGCGTGGCAGTG
GCCGTGGCGGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCCCTGGATGAGGTGGCCATTGATTTCGAAGTGATAGGTGTTTCGCCAC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGTGCTTTCGGCCTGCTCGCCACCCAACTGGC
TGCCTCCCAGGAACGGCTGACTGTGGCGGTTGTAGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGTCGCCAACTGACCGAGGAAATTCAGCGCCGCTATGGTCTGACACCC
GAGCAGGCCGGCCTGGCAAAGAAGCAGGGTGGCCTGCCGGACGATTATCTCAGTGAGGTGCTGCAACCTTTTCGCGAGGC
CCTGGTACAGCAGGTCTCGCGGTCCTTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGATCACATTCTGCTGG
CCGGGGGCTCGGCTTCTGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCCGGTGATGCGCCGGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472