Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMK46_RS24615 Genome accession   NZ_CP125372
Coordinates   5557288..5558988 (+) Length   566 a.a.
NCBI ID   WP_095048681.1    Uniprot ID   -
Organism   Pseudomonas germanica strain P9_87     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5552288..5563988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK46_RS24595 (QMK46_24590) - 5552447..5555395 (+) 2949 WP_320413508.1 DUF748 domain-containing protein -
  QMK46_RS24600 (QMK46_24595) - 5555395..5555691 (+) 297 WP_093434887.1 DUF2845 domain-containing protein -
  QMK46_RS24605 (QMK46_24600) - 5555979..5556335 (-) 357 WP_047600285.1 BON domain-containing protein -
  QMK46_RS24610 (QMK46_24605) - 5556630..5557061 (-) 432 WP_095048680.1 pilin -
  QMK46_RS24615 (QMK46_24610) pilB 5557288..5558988 (+) 1701 WP_095048681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK46_RS24620 (QMK46_24615) pilC 5558991..5560208 (+) 1218 WP_093434875.1 type II secretion system F family protein Machinery gene
  QMK46_RS24625 (QMK46_24620) pilD 5560210..5561082 (+) 873 WP_320413510.1 A24 family peptidase Machinery gene
  QMK46_RS24630 (QMK46_24625) coaE 5561079..5561702 (+) 624 WP_320413511.1 dephospho-CoA kinase -
  QMK46_RS24635 (QMK46_24630) yacG 5561699..5561899 (+) 201 WP_095048684.1 DNA gyrase inhibitor YacG -
  QMK46_RS24640 (QMK46_24635) - 5561906..5562121 (-) 216 WP_007909901.1 hypothetical protein -
  QMK46_RS24645 (QMK46_24640) - 5562187..5562876 (-) 690 WP_095048686.1 energy-coupling factor ABC transporter permease -
  QMK46_RS24650 (QMK46_24645) - 5562873..5563340 (-) 468 WP_095048687.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62640.61 Da        Isoelectric Point: 5.9184

>NTDB_id=831454 QMK46_RS24615 WP_095048681.1 5557288..5558988(+) (pilB) [Pseudomonas germanica strain P9_87]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHAMPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
VDVESVDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=831454 QMK46_RS24615 WP_095048681.1 5557288..5558988(+) (pilB) [Pseudomonas germanica strain P9_87]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
TTGGCAACAGGCCCAACGCAATCGTCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTCACAGGTCG
CCGAGATCGCCTCCGAGCATTTCGGCATGGCCTTGCTTGATCTCAACTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTCGTGCGTCAGCATCACGCCATGCCCTTGTGGCGTCGCGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAAGCAATCAACGACATCCAGTTCAGTACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AACTTACCGATGCCATCGAGAAATTCTTCGACACCCACACCAGCGGTCTGGAGGACATGGCCGATGTCGATCTCGACGGG
GTCGATGTCGAATCCGTCGACGACAGCAAGCAGGACGCCATCGGCGGGCTCGACGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGTTGCTGGATGCGATCAAGAGTGGCTCTTCCGACTTGCACTTCGAGCCTTACGAAAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTTGCCAAACCGCCGATTCAATTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCGGAACGGCGCAAACCTCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGTATCGACGCGCTCGGCTATGAGCCAGAGCAGAAAGACCTGTACATGGCCGCACTCAAGCAGCCACAGGGC
ATGATTCTGGTCACCGGGCCGACCGGTTCGGGTAAAACCGTTTCGCTGTATACCGGCCTGAACATCCTCAACACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GGCTCGACTTTGCCCAGGCACTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGTGATCTG
GAAACTGCCGAGATTGCGATCAAGGCTGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCTGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCTACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAAAAAGCCATCGAGATCCCGCGCGAAACCTTACTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGCTTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACCTGCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGACTGCACAAGGCCATGCAAGGCATCACCAGTCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.156

99.647

0.58
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilF Neisseria gonorrhoeae MS11

49.911

99.47

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.47

0.496
  pilB Vibrio campbellii strain DS40M4

48.09

100

0.489
  pilF Thermus thermophilus HB27

36.918

98.587

0.364