Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   QMK46_RS24620 Genome accession   NZ_CP125372
Coordinates   5558991..5560208 (+) Length   405 a.a.
NCBI ID   WP_093434875.1    Uniprot ID   -
Organism   Pseudomonas germanica strain P9_87     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5553991..5565208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK46_RS24600 (QMK46_24595) - 5555395..5555691 (+) 297 WP_093434887.1 DUF2845 domain-containing protein -
  QMK46_RS24605 (QMK46_24600) - 5555979..5556335 (-) 357 WP_047600285.1 BON domain-containing protein -
  QMK46_RS24610 (QMK46_24605) - 5556630..5557061 (-) 432 WP_095048680.1 pilin -
  QMK46_RS24615 (QMK46_24610) pilB 5557288..5558988 (+) 1701 WP_095048681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK46_RS24620 (QMK46_24615) pilC 5558991..5560208 (+) 1218 WP_093434875.1 type II secretion system F family protein Machinery gene
  QMK46_RS24625 (QMK46_24620) pilD 5560210..5561082 (+) 873 WP_320413510.1 A24 family peptidase Machinery gene
  QMK46_RS24630 (QMK46_24625) coaE 5561079..5561702 (+) 624 WP_320413511.1 dephospho-CoA kinase -
  QMK46_RS24635 (QMK46_24630) yacG 5561699..5561899 (+) 201 WP_095048684.1 DNA gyrase inhibitor YacG -
  QMK46_RS24640 (QMK46_24635) - 5561906..5562121 (-) 216 WP_007909901.1 hypothetical protein -
  QMK46_RS24645 (QMK46_24640) - 5562187..5562876 (-) 690 WP_095048686.1 energy-coupling factor ABC transporter permease -
  QMK46_RS24650 (QMK46_24645) - 5562873..5563340 (-) 468 WP_095048687.1 FAD/FMN-containing dehydrogenase -
  QMK46_RS24655 (QMK46_24650) - 5563471..5564097 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  QMK46_RS24660 (QMK46_24655) - 5564094..5564621 (+) 528 WP_095048688.1 MOSC domain-containing protein -
  QMK46_RS24665 (QMK46_24660) - 5564673..5564846 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43968.65 Da        Isoelectric Point: 10.1093

>NTDB_id=831455 QMK46_RS24620 WP_093434875.1 5558991..5560208(+) (pilC) [Pseudomonas germanica strain P9_87]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKARDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDDVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILAALVAAIFGTR
HALKTSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGVFPSMAVQMTAIGEESGALDDMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=831455 QMK46_RS24620 WP_093434875.1 5558991..5560208(+) (pilC) [Pseudomonas germanica strain P9_87]
ATGGCGGTCAAGGCAGCAAAAATCAGTATTTATGCCTGGGAAGGCACGGACAGAAAAGGCAGCAAAGTCACCGGTGAATT
GAGCGGACAGAACCCGGCGCTGATCAAGGCACAATTGCGTAAACAGGGGATCAATCCCGGCAAGGTGCGCAAGAAATCCG
CGTCATTGTTGAGCTTTGGCAAACGCATCAAGGCCCGGGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTACCGCTGTTGCAGTCGTTCGACATTATTGGCGAAGGCTTTGATAACCCGGCCATGCGCAAACTGGTGGACGA
CGTGAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCCGCAGCCCTGCGCAAGAAACCGCAGTATTTCGACGAGTTGTATT
GCAACCTGGTCGATGCCGGCGAACAGTCCGGCGCCCTCGACACCTTGCTCGAACGCGTCGCGACCTATAAGGAAAAAAGC
GAAAGCCTCAAAGCCAAGATCAAGAAAGCCATGACCTATCCCACCGCCGTGGTGCTGGTCGCGGCTGTTGTCACCGGGAT
TCTGCTGGTCAAAGTAGTGCCACAATTTCAGTCAGTGTTTTCCGGTTTCGGCGCCGAACTGCCGGCGTTCACCCTGATGG
TGATCAGCCTGTCGGAATTCATGCAGCAATGGTGGTGGGCCATTCTCGCTGCGCTGGTCGCGGCCATTTTCGGCACCCGC
CACGCGCTGAAAACCTCCCAGGCACTGCGCGATCGCAAAGACACTTGGCTGCTGAAACTGCCACTGGTGGGCACGCTGAT
GTACAAGTCCGCCGTGGCGCGATTCGCTCGAACCTTGTCCACCACGTTTGCCGCCGGTGTGCCGTTGGTCGAAGCGCTTG
ACTCGGTGGCCGGCGCCACCGGCAACGTGGTCTTCAAGCGAGCCGTGCTGCGCATTCGCCAGGACGTGTCCACCGGTATG
CAGCTGAATTTCTCGATGCGCAGCACCGGGGTCTTTCCCAGCATGGCCGTGCAAATGACCGCGATTGGCGAAGAGTCCGG
CGCCCTGGATGACATGCTCGACAAGGTTGCGGGCTTTTACGAGGAAGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TCATGGAGCCGTTCATCATGGTCGTGCTGGGGGTAATCGTCGGCGGGCTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.309

100

0.753
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

38.653

99.012

0.383