Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OH685_RS04430 Genome accession   NZ_CP125227
Coordinates   901716..903428 (-) Length   570 a.a.
NCBI ID   WP_282463837.1    Uniprot ID   -
Organism   Acinetobacter pittii strain SO1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 896716..908428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH685_RS04400 (OH685_04435) - 896880..897206 (+) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  OH685_RS04405 (OH685_04440) rlmB 897309..898058 (+) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  OH685_RS04410 (OH685_04445) - 898094..899008 (+) 915 WP_282463835.1 DMT family transporter -
  OH685_RS04415 (OH685_04450) coaE 899005..899601 (-) 597 WP_005065869.1 dephospho-CoA kinase -
  OH685_RS04420 (OH685_04455) pilD 899603..900463 (-) 861 WP_282463836.1 A24 family peptidase Machinery gene
  OH685_RS04425 (OH685_04460) pilC 900463..901689 (-) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  OH685_RS04430 (OH685_04465) pilB 901716..903428 (-) 1713 WP_282463837.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OH685_RS04435 (OH685_04470) tpiA 903720..904514 (+) 795 WP_282463838.1 triose-phosphate isomerase -
  OH685_RS04440 (OH685_04475) secG 904527..904856 (+) 330 WP_032005670.1 preprotein translocase subunit SecG -
  OH685_RS04460 (OH685_04495) rimP 905494..906018 (+) 525 WP_002114922.1 ribosome maturation factor RimP -
  OH685_RS04465 (OH685_04500) nusA 906056..907540 (+) 1485 WP_002115134.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63529.90 Da        Isoelectric Point: 5.0294

>NTDB_id=830443 OH685_RS04430 WP_282463837.1 901716..903428(-) (pilB) [Acinetobacter pittii strain SO1]
MSVLNTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=830443 OH685_RS04430 WP_282463837.1 901716..903428(-) (pilB) [Acinetobacter pittii strain SO1]
ATGTCAGTCTTAAATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCAGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGACCAGAAACTTGTTTTAAAACATCGGGTCTTACCTCTTATACAGAGAGGACAGATTTTATATGT
CGCGACCAGCAACCCGAGTAATATTGAAGCGATTGATGCTATTCGCTTCAATAGCAAGCTCATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGAAGCTCCCCCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCTATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTTCGTTATCGAGTCGATGGCGTGTTACGTCAGATTGCAAATCCGCCTTTGCAATTGGCCAACCGTTTGGCTTCA
CGTTTGAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGATGGACGTATCAAGCTTAAATTATCAAA
AACTAAAGCCATCGATTTTCGTGTGAACTCACTTCCAACTTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGTGCAATGCTCGGCATTGATGCTTTAGGATATGAAGAAGATCAAAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTTAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.088

100

0.951
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

50.957

100

0.514
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

36.833

98.596

0.363