Detailed information NTG0828380

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QKW11_RS12820
• Genome accession: NZ_CP124851
• Coordinates: 2647818..2648432 (-)
• Length: 204 a.a.
• NCBI ID: WP_284064731.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SF305
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2642818..2653432

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QKW11_RS12790 (QKW11_12785)	rpsT	2643121..2643387 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
QKW11_RS12795 (QKW11_12790)	holA	2643404..2644445 (-)	1042	Protein_2474	DNA polymerase III subunit delta	-
QKW11_RS12800 (QKW11_12795)	-	2644485..2644636 (-)	152	Protein_2475	hypothetical protein	-
QKW11_RS12805 (QKW11_12800)	-	2644677..2644811 (+)	135	WP_003152870.1	YqzM family protein	-
QKW11_RS12810 (QKW11_12805)	comEC	2644830..2647181 (-)	2352	WP_256683591.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QKW11_RS12815 (QKW11_12810)	-	2647182..2647751 (-)	570	WP_003152868.1	ComE operon protein 2	-
QKW11_RS12820 (QKW11_12815)	comEA	2647818..2648432 (-)	615	WP_284064731.1	helix-hairpin-helix domain-containing protein	Machinery gene
QKW11_RS12825 (QKW11_12820)	comER	2648491..2649312 (+)	822	WP_064107158.1	late competence protein ComER	-
QKW11_RS12830 (QKW11_12825)	-	2649381..2650118 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
QKW11_RS12835 (QKW11_12830)	rsfS	2650115..2650471 (-)	357	WP_007408260.1	ribosome silencing factor	-
QKW11_RS12840 (QKW11_12835)	yqeK	2650489..2651049 (-)	561	WP_007408259.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QKW11_RS12845 (QKW11_12840)	-	2651039..2651608 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
QKW11_RS12850 (QKW11_12845)	yhbY	2651619..2651909 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
QKW11_RS12855 (QKW11_12850)	aroE	2651903..2652745 (-)	843	WP_033574150.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22235.45 Da
• Isoelectric Point: 6.4888

>NTDB_id=828380 QKW11_RS12820 WP_284064731.1 2647818..2648432(-) (comEA) [Bacillus velezensis strain SF305]
MTKAMMTERLRRNLKILVACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=828380 QKW11_RS12820 WP_284064731.1 2647818..2648432(-) (comEA) [Bacillus velezensis strain SF305]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.653	96.078	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA	Lactococcus lactis subsp. cremoris KW2	34.685	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363