Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PMI20_RS28910 Genome accession   NZ_CP124732
Coordinates   6448665..6449882 (+) Length   405 a.a.
NCBI ID   WP_007924674.1    Uniprot ID   -
Organism   Pseudomonas sp. GM17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6443665..6454882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMI20_RS28895 (PMI20_028895) - 6444120..6447038 (+) 2919 WP_007924671.1 DUF748 domain-containing protein -
  PMI20_RS28900 (PMI20_028900) - 6447242..6447598 (-) 357 WP_007924672.1 BON domain-containing protein -
  PMI20_RS28905 (PMI20_028905) - 6447885..6448301 (-) 417 WP_007924673.1 pilin -
  PMI20_RS28910 (PMI20_028910) pilC 6448665..6449882 (+) 1218 WP_007924674.1 type II secretion system F family protein Machinery gene
  PMI20_RS28915 (PMI20_028915) pilD 6449885..6450757 (+) 873 WP_007924675.1 A24 family peptidase Machinery gene
  PMI20_RS28920 (PMI20_028920) coaE 6450754..6451377 (+) 624 WP_007924677.1 dephospho-CoA kinase -
  PMI20_RS28925 (PMI20_028925) yacG 6451374..6451577 (+) 204 WP_007924679.1 DNA gyrase inhibitor YacG -
  PMI20_RS28930 (PMI20_028930) - 6451582..6451797 (-) 216 WP_007924682.1 hypothetical protein -
  PMI20_RS28935 (PMI20_028935) - 6451863..6452552 (-) 690 WP_007924684.1 energy-coupling factor ABC transporter permease -
  PMI20_RS28940 (PMI20_028940) - 6452549..6453016 (-) 468 WP_007924686.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44508.44 Da        Isoelectric Point: 10.3403

>NTDB_id=827793 PMI20_RS28910 WP_007924674.1 6448665..6449882(+) (pilC) [Pseudomonas sp. GM17]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGINPARVRKKSSSLLQRTPPIKACDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMGELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPMFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HARRRSPRFRHWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLMEALGSVAGATGNQVFKQAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=827793 PMI20_RS28910 WP_007924674.1 6448665..6449882(+) (pilC) [Pseudomonas sp. GM17]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAACCCGGCCAGGGTCAGGAAGAAGTCCA
GCTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCTGCGACATCAGCCTGTTCACCCGGCAACTGGCGACCCTGATCAAG
GCCGGCGTGCCACTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACCGCGCTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGGATCCGCAAGGCCATGACTTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAGGGCTTCGGCGCGCAATTGCCCATGTTCACGCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCACTGTTGCTCAGTGTGTTCGGCCTGCGC
CATGCCCGCCGGCGCTCACCACGCTTTCGCCACTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCACTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACTTTCGCCGCCGGAGTGCCGCTGATGGAAGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCAAGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGCGCATCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCATTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.938

100

0.649
  pilC Acinetobacter baylyi ADP1

56.127

100

0.565
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

42.184

99.506

0.42
  pilG Neisseria meningitidis 44/76-A

41.439

99.506

0.412
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilC Vibrio campbellii strain DS40M4

39.055

99.259

0.388