Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PMI20_RS05575 Genome accession   NZ_CP124732
Coordinates   1238712..1240784 (+) Length   690 a.a.
NCBI ID   WP_007925845.1    Uniprot ID   -
Organism   Pseudomonas sp. GM17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1233712..1245784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMI20_RS05555 (PMI20_005555) pilM 1235942..1236994 (+) 1053 WP_007925849.1 pilus assembly protein PilM Machinery gene
  PMI20_RS05560 (PMI20_005560) - 1236994..1237560 (+) 567 WP_007925848.1 PilN domain-containing protein -
  PMI20_RS05565 (PMI20_005565) pilO 1237557..1238174 (+) 618 WP_007925847.1 type 4a pilus biogenesis protein PilO -
  PMI20_RS05570 (PMI20_005570) - 1238171..1238698 (+) 528 WP_007925846.1 pilus assembly protein PilP -
  PMI20_RS05575 (PMI20_005575) pilQ 1238712..1240784 (+) 2073 WP_007925845.1 type IV pilus secretin PilQ Machinery gene
  PMI20_RS05580 (PMI20_005580) aroK 1240789..1241307 (+) 519 WP_009041824.1 shikimate kinase AroK -
  PMI20_RS05585 (PMI20_005585) aroB 1241456..1242556 (+) 1101 WP_007925843.1 3-dehydroquinate synthase -
  PMI20_RS05590 (PMI20_005590) - 1242567..1244156 (+) 1590 WP_007925840.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74682.89 Da        Isoelectric Point: 5.4337

>NTDB_id=827781 PMI20_RS05575 WP_007925845.1 1238712..1240784(+) (pilQ) [Pseudomonas sp. GM17]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVISQLADKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAALSAPGQPAVASGRAKAVASVGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERGGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDRSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNVSGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=827781 PMI20_RS05575 WP_007925845.1 1238712..1240784(+) (pilQ) [Pseudomonas sp. GM17]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCGCTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCGCGCG
GCTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCCGGTGTTATCAGCCAGCTGGCAGACAAGAGCCGTGAC
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTCGTCGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGGGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCTGAGTGCGC
CGGGGCAGCCGGCTGTCGCGTCCGGGCGGGCCAAGGCCGTGGCTTCCGTGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAAGGCGAAGGTAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATACAGGAGCGCGGCGG
CAAGCTCGTCCTCACCTTCGCCAAGGCCCGCCTGCCCGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGATTTCGCCACCC
CCGTGCAGTTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACTTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTGAGCGTCAGGCCGATGACCTCGGGCGACCTGCAGAAGCGCAACGCCGAGCG
CTCGGCCTACAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGATT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAACGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCTGCCCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCATTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCCGAGGACAGGTCCGACGAACGC
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGTCTGGACGAGCTGCGGCGGAT
CGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAAGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACCAGGGCAACTGGAATGTCTCGGGGGTCAGCAACGGCACCAACGGT
TCCTCCACCATAGGCACGCCGGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACTACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAATGTGTTGCTGGACCTTGAGCTCACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCACAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAAGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGATGGAGAGACCATCGTCATCGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)

AMIN domain (AMIN)

(40-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

40.192

100

0.425
  pilQ Acinetobacter baumannii strain A118

38.338

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.338

100

0.414
  pilQ Legionella pneumophila strain Lp02

38.336

97.536

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.416

96.957

0.372