Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PMI20_RS05555 Genome accession   NZ_CP124732
Coordinates   1235942..1236994 (+) Length   350 a.a.
NCBI ID   WP_007925849.1    Uniprot ID   -
Organism   Pseudomonas sp. GM17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1230942..1241994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMI20_RS05545 (PMI20_005545) - 1231886..1233154 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  PMI20_RS05550 (PMI20_005550) - 1233290..1235725 (-) 2436 WP_172963018.1 penicillin-binding protein 1A -
  PMI20_RS05555 (PMI20_005555) pilM 1235942..1236994 (+) 1053 WP_007925849.1 pilus assembly protein PilM Machinery gene
  PMI20_RS05560 (PMI20_005560) - 1236994..1237560 (+) 567 WP_007925848.1 PilN domain-containing protein -
  PMI20_RS05565 (PMI20_005565) pilO 1237557..1238174 (+) 618 WP_007925847.1 type 4a pilus biogenesis protein PilO -
  PMI20_RS05570 (PMI20_005570) - 1238171..1238698 (+) 528 WP_007925846.1 pilus assembly protein PilP -
  PMI20_RS05575 (PMI20_005575) pilQ 1238712..1240784 (+) 2073 WP_007925845.1 type IV pilus secretin PilQ Machinery gene
  PMI20_RS05580 (PMI20_005580) aroK 1240789..1241307 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37022.44 Da        Isoelectric Point: 4.9975

>NTDB_id=827780 PMI20_RS05555 WP_007925849.1 1235942..1236994(+) (pilM) [Pseudomonas sp. GM17]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLAARVVDLEVCALERSFALAAGSPAARTVALVDIGATLTTLSIVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASAPQHQVDSILLAGGSASVPGLVPLIEQRLGLPALIANPFAGM
SIGAKVDAAALVSEAPSLMVACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=827780 PMI20_RS05555 WP_007925849.1 1235942..1236994(+) (pilM) [Pseudomonas sp. GM17]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGTTTTCGGGTCGAGGCCTATGGCGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCTGAAGGGGTCGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCCC
GCAATCCCGAGCGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAAGTTCGCGAGGCGGCGCTCGAGCTC
GCCGGGCTGGCGGCCCGAGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGCTCGCCGGC
TGCCCGGACCGTGGCGCTGGTCGATATCGGCGCCACCCTGACCACCCTGAGCATCGTGCATGAAGGGCAGATCGTCTATT
CCCGTGAGCAGTTGTTCGGCGGACACCAACTGACCGAACACATCCAGCGTCGCTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAGAAGCAGGGTGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCTTTTCAGGAGGCGGTTGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACACCAGGTCGACTCCATCCTGTTGGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGAGCAGCGCTTGGGCTTGCCTGCGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGCCAAGGTCGACGCCGCGGCCCTGGTCAGCGAAGCCCCGTCCTTGATGGTCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

51.705

100

0.52
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46