Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QKA38_RS24960 Genome accession   NZ_CP124669
Coordinates   5356736..5358436 (+) Length   566 a.a.
NCBI ID   WP_023098060.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2021CK-01494     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5351736..5363436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QKA38_RS24940 (QKA38_24940) nadC 5353551..5354399 (+) 849 WP_023098057.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QKA38_RS24950 (QKA38_24950) - 5354580..5355959 (-) 1380 WP_226342245.1 O-antigen ligase family protein -
  QKA38_RS24955 (QKA38_24955) - 5356041..5356505 (-) 465 WP_023098059.1 pilin -
  QKA38_RS24960 (QKA38_24960) pilB 5356736..5358436 (+) 1701 WP_023098060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QKA38_RS24965 (QKA38_24965) pilC 5358440..5359657 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  QKA38_RS24970 (QKA38_24970) pilD 5359658..5360530 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  QKA38_RS24975 (QKA38_24975) coaE 5360527..5361138 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  QKA38_RS24980 (QKA38_24980) yacG 5361135..5361335 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  QKA38_RS24985 (QKA38_24985) - 5361372..5361581 (-) 210 WP_003094660.1 hypothetical protein -
  QKA38_RS24990 (QKA38_24990) - 5361687..5362376 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  QKA38_RS24995 (QKA38_24995) - 5362373..5362843 (-) 471 WP_023098062.1 hypothetical protein -
  QKA38_RS25000 (QKA38_25000) - 5362840..5363265 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62324.22 Da        Isoelectric Point: 5.5681

>NTDB_id=827592 QKA38_RS24960 WP_023098060.1 5356736..5358436(+) (pilB) [Pseudomonas aeruginosa strain 2021CK-01494]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
AISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASHEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=827592 QKA38_RS24960 WP_023098060.1 5356736..5358436(+) (pilB) [Pseudomonas aeruginosa strain 2021CK-01494]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTAGCCGCCGAGCAGTTTGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GCCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGATGCCCTCGGCGATCTCGGCGACATCGATCTCGAT
GGAGTCGATGTGGGCAAGGCAGAAAGCGCCAGCCATGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGGCTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTTATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTATCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAG
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.641

100

0.551
  pilB Vibrio cholerae strain A1552

51.699

98.763

0.511
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.345

94.17

0.493
  pilB Vibrio campbellii strain DS40M4

51.407

94.17

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.84

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378