Detailed information NTG0826437

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: SE698_RS03445
• Genome accession: NZ_CP138428
• Coordinates: 730410..731795 (-)
• Length: 461 a.a.
• NCBI ID: WP_001025193.1
• Uniprot ID: -
• Organism: Escherichia coli strain 6EVA
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 725410..736795

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SE698_RS03410	mutT	725740..726129 (+)	390	WP_000736009.1	8-oxo-dGTP diphosphatase MutT	-
SE698_RS03415	yacG	726345..726542 (-)	198	WP_000005042.1	DNA gyrase inhibitor YacG	-
SE698_RS03420	zapD	726552..727295 (-)	744	WP_001194734.1	cell division protein ZapD	-
SE698_RS03425	coaE	727295..727915 (-)	621	WP_001269520.1	dephospho-CoA kinase	-
SE698_RS03430	-	727940..727984 (+)	45	WP_120795372.1	protein YacM	-
SE698_RS03435	guaC	728140..729183 (+)	1044	WP_319054624.1	GMP reductase	-
SE698_RS03440	hofC	729218..730420 (-)	1203	WP_000157259.1	protein transport protein HofC	-
SE698_RS03445	pilB	730410..731795 (-)	1386	WP_001025193.1	type II secretion system protein GspE	Machinery gene
SE698_RS03450	ppdD	731805..732245 (-)	441	WP_000360900.1	prepilin peptidase-dependent pilin	-
SE698_RS03455	nadC	732448..733341 (-)	894	WP_001135168.1	carboxylating nicotinate-nucleotide diphosphorylase	-
SE698_RS03460	ampD	733429..733980 (+)	552	WP_000923727.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
SE698_RS03465	ampE	733977..734831 (+)	855	WP_000172005.1	beta-lactamase regulator AmpE	-
SE698_RS03470	aroP	734874..736247 (-)	1374	WP_000969912.1	aromatic amino acid transporter AroP	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 50623.30 Da
• Isoelectric Point: 6.5396

>NTDB_id=826437 SE698_RS03445 WP_001025193.1 730410..731795(-) (pilB) [Escherichia coli strain 6EVA]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCIHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide

• Length: 1386 bp

>NTDB_id=826437 SE698_RS03445 WP_001025193.1 730410..731795(-) (pilB) [Escherichia coli strain 6EVA]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTGTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTATACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (88-353)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (8-68)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	50	83.297	0.416
pilB	Glaesserella parasuis strain SC1401	41.253	100	0.414
pilB	Acinetobacter baylyi ADP1	40.171	100	0.408
pilB	Vibrio campbellii strain DS40M4	48.446	83.731	0.406
pilB	Vibrio cholerae strain A1552	46.667	84.599	0.395
pilB	Vibrio parahaemolyticus RIMD 2210633	46.41	84.599	0.393
pilB	Haemophilus influenzae 86-028NP	45.22	83.948	0.38
pilB	Acinetobacter baumannii D1279779	43.909	85.466	0.375
pilB	Haemophilus influenzae Rd KW20	44.444	83.948	0.373
pilF	Neisseria gonorrhoeae MS11	44.416	83.514	0.371
pilF	Thermus thermophilus HB27	40.587	88.72	0.36