Detailed information NTG0826346

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: SE700_RS01750
• Genome accession: NZ_CP138427
• Coordinates: 190730..192115 (+)
• Length: 461 a.a.
• NCBI ID: WP_053881825.1
• Uniprot ID: -
• Organism: Escherichia coli strain 21EVA
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 185730..197115

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SE700_RS01725	aroP	186278..187651 (+)	1374	WP_000969912.1	aromatic amino acid transporter AroP	-
SE700_RS01730	ampE	187694..188548 (-)	855	WP_000172014.1	beta-lactamase regulator AmpE	-
SE700_RS01735	ampD	188545..189096 (-)	552	WP_000923721.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
SE700_RS01740	nadC	189184..190077 (+)	894	WP_001135176.1	carboxylating nicotinate-nucleotide diphosphorylase	-
SE700_RS01745	pilA	190280..190720 (+)	441	WP_000360904.1	prepilin peptidase-dependent pilin	Machinery gene
SE700_RS01750	pilB	190730..192115 (+)	1386	WP_053881825.1	type II secretion system protein GspE	Machinery gene
SE700_RS01755	hofC	192105..193307 (+)	1203	WP_319791338.1	protein transport protein HofC	-
SE700_RS01760	guaC	193342..194385 (-)	1044	WP_001217338.1	GMP reductase	-
SE700_RS01765	-	194541..194585 (-)	45	WP_120795372.1	protein YacM	-
SE700_RS01770	coaE	194610..195230 (+)	621	WP_001269527.1	dephospho-CoA kinase	-
SE700_RS01775	zapD	195230..195973 (+)	744	WP_001194722.1	cell division protein ZapD	-
SE700_RS01780	yacG	195983..196180 (+)	198	WP_000005042.1	DNA gyrase inhibitor YacG	-
SE700_RS01785	mutT	196396..196785 (-)	390	WP_000736009.1	8-oxo-dGTP diphosphatase MutT	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 50619.30 Da
• Isoelectric Point: 6.5796

>NTDB_id=826346 SE700_RS01750 WP_053881825.1 190730..192115(+) (pilB) [Escherichia coli strain 21EVA]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGELIHIPDTVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide

• Length: 1386 bp

>NTDB_id=826346 SE700_RS01750 WP_053881825.1 190730..192115(+) (pilB) [Escherichia coli strain 21EVA]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CAGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGTGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCTCATCCATATTCCAGACACTGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (8-68)

• Type II/IV secretion system protein (T2SSE) (88-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio campbellii strain DS40M4	42.443	100	0.445
pilB	Legionella pneumophila strain ERS1305867	50.773	84.165	0.427
pilB	Glaesserella parasuis strain SC1401	41.253	100	0.414
pilB	Acinetobacter baylyi ADP1	39.655	100	0.399
pilB	Vibrio cholerae strain A1552	46.667	84.599	0.395
pilB	Vibrio parahaemolyticus RIMD 2210633	46.753	83.514	0.39
pilB	Haemophilus influenzae 86-028NP	45.478	83.948	0.382
pilB	Haemophilus influenzae Rd KW20	44.703	83.948	0.375
pilB	Acinetobacter baumannii D1279779	43.846	84.599	0.371
pilF	Neisseria gonorrhoeae MS11	44.156	83.514	0.369
pilF	Thermus thermophilus HB27	41.542	87.202	0.362