Detailed information NTG0825925

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: SE699_RS20790
• Genome accession: NZ_CP138399
• Coordinates: 4259546..4260931 (+)
• Length: 461 a.a.
• NCBI ID: WP_001025199.1
• Uniprot ID: -
• Organism: Escherichia coli strain 64EVA
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4254546..4265931

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SE699_RS20765	aroP	4255095..4256468 (+)	1374	WP_000969912.1	aromatic amino acid transporter AroP	-
SE699_RS20770	ampE	4256511..4257365 (-)	855	WP_000172014.1	beta-lactamase regulator AmpE	-
SE699_RS20775	ampD	4257362..4257913 (-)	552	WP_000923721.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
SE699_RS20780	nadC	4258001..4258894 (+)	894	WP_001135174.1	carboxylating nicotinate-nucleotide diphosphorylase	-
SE699_RS20785	ppdD	4259097..4259536 (+)	440	Protein_4039	prepilin peptidase-dependent pilin	-
SE699_RS20790	pilB	4259546..4260931 (+)	1386	WP_001025199.1	type II secretion system protein GspE	Machinery gene
SE699_RS20795	hofC	4260921..4262123 (+)	1203	WP_000157262.1	protein transport protein HofC	-
SE699_RS20800	guaC	4262158..4263201 (-)	1044	WP_001217338.1	GMP reductase	-
SE699_RS20805	-	4263357..4263401 (-)	45	WP_120795372.1	protein YacM	-
SE699_RS20810	coaE	4263426..4264046 (+)	621	WP_001269527.1	dephospho-CoA kinase	-
SE699_RS20815	zapD	4264046..4264789 (+)	744	WP_001194722.1	cell division protein ZapD	-
SE699_RS20820	yacG	4264799..4264996 (+)	198	WP_000005042.1	DNA gyrase inhibitor YacG	-
SE699_RS20825	mutT	4265212..4265601 (-)	390	WP_000736009.1	8-oxo-dGTP diphosphatase MutT	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 50610.29 Da
• Isoelectric Point: 6.5405

>NTDB_id=825925 SE699_RS20790 WP_001025199.1 4259546..4260931(+) (pilB) [Escherichia coli strain 64EVA]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGELIHIPDTVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide

• Length: 1386 bp

>NTDB_id=825925 SE699_RS20790 WP_001025199.1 4259546..4260931(+) (pilB) [Escherichia coli strain 64EVA]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGTGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCTCATCCATATTCCAGACACTGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (8-68)

• Type II/IV secretion system protein (T2SSE) (88-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	50.773	84.165	0.427
pilB	Glaesserella parasuis strain SC1401	41.253	100	0.414
pilB	Vibrio campbellii strain DS40M4	48.187	83.731	0.403
pilB	Acinetobacter baylyi ADP1	39.655	100	0.399
pilB	Vibrio cholerae strain A1552	46.667	84.599	0.395
pilB	Vibrio parahaemolyticus RIMD 2210633	46.753	83.514	0.39
pilB	Haemophilus influenzae 86-028NP	45.478	83.948	0.382
pilB	Haemophilus influenzae Rd KW20	44.703	83.948	0.375
pilB	Acinetobacter baumannii D1279779	43.846	84.599	0.371
pilF	Neisseria gonorrhoeae MS11	44.156	83.514	0.369
pilF	Thermus thermophilus HB27	41.542	87.202	0.362