Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QFW95_RS25370 Genome accession   NZ_CP124219
Coordinates   5699856..5701928 (-) Length   690 a.a.
NCBI ID   WP_047225964.1    Uniprot ID   -
Organism   Pseudomonas sp. P13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5694856..5706928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFW95_RS25355 - 5696512..5698074 (-) 1563 WP_281459620.1 AAA family ATPase -
  QFW95_RS25360 aroB 5698085..5699185 (-) 1101 WP_047225963.1 3-dehydroquinate synthase -
  QFW95_RS25365 aroK 5699333..5699851 (-) 519 WP_003196983.1 shikimate kinase AroK -
  QFW95_RS25370 pilQ 5699856..5701928 (-) 2073 WP_047225964.1 type IV pilus secretin PilQ Machinery gene
  QFW95_RS25375 - 5701925..5702470 (-) 546 WP_160893798.1 pilus assembly protein PilP -
  QFW95_RS25380 pilO 5702467..5703090 (-) 624 WP_047225966.1 type 4a pilus biogenesis protein PilO -
  QFW95_RS25385 - 5703087..5703653 (-) 567 WP_047225967.1 PilN domain-containing protein -
  QFW95_RS25390 comM 5703653..5704717 (-) 1065 WP_047225968.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74611.80 Da        Isoelectric Point: 5.4013

>NTDB_id=823986 QFW95_RS25370 WP_047225964.1 5699856..5701928(-) (pilQ) [Pseudomonas sp. P13]
MNRFFSALGISLWIALLSPMVHAVNLKTLDVAALPGDRIELKLTFDGPPPAPRGYTTEQPARIALDLPGVTSQLAHKRHD
LGAGNARSATVVEAADRTRLIIGLTQLAPYTARVEGNSLLVVVGKGAVASATAQAPRPAAPSAVPTRASAPVGKAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFAKTRLPEPLRVRLDVKDFATPVQFVNASAGADRATISIEPSGTFD
YSTYQTDNKLTVSVRPMTVEDLQKRDASREAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVTTGD
STTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEQKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=823986 QFW95_RS25370 WP_047225964.1 5699856..5701928(-) (pilQ) [Pseudomonas sp. P13]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGCGGTCAATCTGAA
AACGCTGGATGTCGCGGCGCTGCCGGGGGACCGCATCGAGTTGAAACTCACCTTCGACGGGCCGCCCCCTGCGCCTCGGG
GCTACACGACCGAGCAGCCGGCCCGGATCGCTCTGGATCTGCCGGGGGTGACCAGCCAGTTGGCCCACAAGAGACATGAT
CTTGGTGCTGGCAATGCGCGAAGCGCCACGGTGGTCGAGGCGGCTGATCGTACCCGTCTGATCATTGGCCTTACCCAGCT
TGCGCCCTATACCGCGCGGGTGGAGGGCAACAGCCTGCTCGTGGTGGTCGGCAAGGGAGCGGTTGCCAGCGCTACTGCCC
AGGCGCCACGCCCGGCGGCTCCCAGCGCTGTGCCCACCAGAGCCAGTGCGCCGGTGGGCAAGGCGATCCGAGGGGTAGAT
TTCCAGCGCGGCACCCAGGGCGAAGGCAACGTGGTGATCGATCTGTCGGACCCCTCGATCGCCCCGGACATCCAGGAGCG
TGAAGGCAAGATCATCCTCAACTTCGCCAAGACCCGGTTACCCGAACCGCTGCGCGTGCGCCTGGACGTCAAGGACTTCG
CCACCCCGGTGCAGTTCGTCAATGCCAGTGCCGGCGCCGACCGGGCCACCATCAGCATCGAACCCAGCGGTACTTTCGAT
TACTCCACCTACCAGACGGACAACAAACTGACCGTCAGTGTCCGACCCATGACAGTCGAGGATCTGCAAAAACGCGATGC
CAGTCGCGAGGCCTACAGCGGTGAAAAGCTGTCCCTCAACTTTCAGGACATCGAGGTGCGCTCGGTCCTGCAACTGATTG
CCGATTTCACCAACCTCAACCTGGTGGCCAGTGACACGGTGCAGGGCGGCATTACCCTGCGCCTGCAAAATGTGCCTTGG
GACCAGGCGCTGGACCTGGTGCTCAAGACCAAAGGGCTGGACAAGCGCAAGGTCGGCAATGTGCTGCTGGTCGCCCCCGC
CGACGAAATCGCGGCCCGTGAGCGCCAGGAACTCGAATCCCAGAAGCAGATTGCCGACCTGGCGCCGCTACGGCGTGAGC
TGTTGCAGGTCAACTATGCGAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAAGCCAAGGCCGAT
GAGCGCGGTACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAACTGCG
CCGGATCGTGGCGCAGCTAGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAACGTTGACTACG
ACAAGAGCCTGGGCGTGCGCTGGGGTGGATCGATCCAGAACAAAGGCAACTGGAACACTTCAGGGGTCACCACCGGCGAC
TCCACCACCATCGGCACGCCGGGCAGCACCAGCACCAACCAGCCGTTTGTCGATCTGGGCGCGTCGGGCAATACCTCCGG
CATCGGCATCGCCTTCATCACCGACAATGTACTGCTGGACCTGGAATTGACGGCCATGGAGAAAACCGGCAACGGGGAAA
TCGTCTCTCAGCCCAAGGTGGTGACGTCCGACAAAGAGACGGCAAAAATCCTCAAGGGCACGGAGATTCCCTATCAGGAA
GCCAGTTCCAGTGGTGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCTCTGGAGGTGACCCCGCAGATCACCCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGATTACCTGAACAAGGTGAATGATGTGCCGCCAATCA
AGAAAAACGAAGTCAACGCCAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACTCAG
AGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GCAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.448

100

0.751
  pilQ Acinetobacter baumannii strain A118

38.784

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.784

100

0.416
  comQ Acinetobacter baylyi ADP1

41.245

100

0.413
  pilQ Legionella pneumophila strain Lp02

37.926

97.826

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.926

97.826

0.371