Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   QFW95_RS25390 Genome accession   NZ_CP124219
Coordinates   5703653..5704717 (-) Length   354 a.a.
NCBI ID   WP_047225968.1    Uniprot ID   A0A1H8K2I6
Organism   Pseudomonas sp. P13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5698653..5709717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFW95_RS25365 aroK 5699333..5699851 (-) 519 WP_003196983.1 shikimate kinase AroK -
  QFW95_RS25370 pilQ 5699856..5701928 (-) 2073 WP_047225964.1 type IV pilus secretin PilQ Machinery gene
  QFW95_RS25375 - 5701925..5702470 (-) 546 WP_160893798.1 pilus assembly protein PilP -
  QFW95_RS25380 pilO 5702467..5703090 (-) 624 WP_047225966.1 type 4a pilus biogenesis protein PilO -
  QFW95_RS25385 - 5703087..5703653 (-) 567 WP_047225967.1 PilN domain-containing protein -
  QFW95_RS25390 comM 5703653..5704717 (-) 1065 WP_047225968.1 pilus assembly protein PilM Machinery gene
  QFW95_RS25395 - 5704925..5707369 (+) 2445 WP_173407570.1 penicillin-binding protein 1A -
  QFW95_RS25400 - 5707626..5708894 (-) 1269 WP_047225970.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38065.58 Da        Isoelectric Point: 4.9070

>NTDB_id=823987 QFW95_RS25390 WP_047225968.1 5703653..5704717(-) (comM) [Pseudomonas sp. P13]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKARTPLRNVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVLGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSP
EQAGQAKKQGGLPDDYLGEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FSNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=823987 QFW95_RS25390 WP_047225968.1 5703653..5704717(-) (comM) [Pseudomonas sp. P13]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTTTGGGGATAGACATCAGTTCCACGTCGGTGAAGCTGCTCGAGTT
GAGCCGCCAGGGTGACCGCTACCGTGTCGAGTCTTACGCGGTCGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAGGGCGTGGGCCAGGCATTGTCACGGGTGCTCGCCAAGGCCAGAACACCCTTGCGTAACGTGGCGGTG
GCTGTAGCGGGGTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGACTTTCCGACGACGACATGGAAAACCAGCT
CAAGATCGAAGCCGATCAATACATTCCTTATCCGCTGGACGAGGTGGCCATCGACTTCGAAGTCCTGGGCGTTTCACCGC
GCAGCGCCGAGCGGGTCGAGGTGCTGTTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAGGCTGCGCTGGCGCTC
GCCGGGCTTACGGCCAAGGTGGTCGATGTGGAGGCCTACGCGCTGGAGCGCGCCTTCGGGCTGCTTGCCACGCAACTGGC
CGCGTCCCAGGAGCGGCTGACGGTGGCGGTGATCGACATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGTCGCCAGCTCACCGAAGAAATCCAGCGCCGCTACGGCCTGTCACCC
GAGCAGGCCGGGCAGGCAAAGAAGCAGGGTGGCCTGCCGGACGATTACCTCGGTGAGGTCCTGCAACCTTTTCGCGAGGC
CCTGGTGCAACAGGTATCGAGGTCCTTGCAGTTTTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGACCACATCCTCTTGG
CTGGAGGCACCGCCTCGGTCGCAGGCCTGGACCGGCTGATCGAACAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTTCCAACATGGCCCTGGGCAGCAAGGTCAACGCCGGTGCCCTGGCCAGTGACGCACCGGCCTTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H8K2I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48