Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QFW95_RS11370 Genome accession   NZ_CP124219
Coordinates   2527506..2528624 (+) Length   372 a.a.
NCBI ID   WP_047227842.1    Uniprot ID   A0A1N7EMY1
Organism   Pseudomonas sp. P13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2522506..2533624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QFW95_RS11355 - 2523549..2524472 (+) 924 WP_076384044.1 sugar kinase -
  QFW95_RS11360 - 2524510..2524785 (-) 276 WP_076384045.1 peptidylprolyl isomerase -
  QFW95_RS11365 - 2524834..2527371 (-) 2538 WP_281460032.1 PAS domain-containing protein -
  QFW95_RS11370 pilU 2527506..2528624 (+) 1119 WP_047227842.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QFW95_RS11375 - 2528790..2530745 (-) 1956 WP_281460033.1 acetoacetate--CoA ligase -
  QFW95_RS11380 hbdH 2530949..2531725 (-) 777 WP_076384048.1 3-hydroxybutyrate dehydrogenase -
  QFW95_RS11385 - 2531734..2533125 (-) 1392 WP_047227845.1 GntP family permease -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41346.26 Da        Isoelectric Point: 6.0188

>NTDB_id=823972 QFW95_RS11370 WP_047227842.1 2527506..2528624(+) (pilU) [Pseudomonas sp. P13]
MEIDPLLRILASQDGSDLYMSTGAPPCARFEGVLKPLSNQPFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPNVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNEFGELKGIMEKSEELGMQTFDHAL
YNLVVEGAIDEEEALKNADSANNLRLRLKLHAESGAAVPPADPAAGEWGLMD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=823972 QFW95_RS11370 WP_047227842.1 2527506..2528624(+) (pilU) [Pseudomonas sp. P13]
ATGGAAATCGATCCCTTGTTACGCATCCTGGCGAGCCAGGATGGCTCCGACCTCTATATGTCCACCGGTGCGCCGCCGTG
CGCGCGTTTCGAGGGGGTGCTCAAACCCTTGAGCAACCAGCCCTTCAAGGTGGGCGACATCGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGGCTTGAGTTCGATCGAGAACTGGAAATGAACCTGGCTATCTCCCTGGCTGGGGTCGGGCGG
TTTCGCGTCAATATCTTCAAGCAGCGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTCGACATCCCTCGGTTCGA
GGACCTGAAACTGCCAAATGTGTTGCTGGACACCATCATGCAGAAACAGGGGCTGATGCTGTTCGTCGGCGCGACAGGGT
CGGGCAAGTCGACTTCCCTGGCGGCGCTGATCGATTACCGTAATCGCAACAGCAGCGGCCATATCATCACCATTGAAGAT
CCGGTGGAGTATATCCATCGACACAAGAAATCGATCATCAATCAACGGGAAGTCGGTGTCGACACCCGCAGCTTTCATGC
CGCCCTGAAGAACACCTTGCGCCAGGCACCAGACGTGGTACTGATCGGCGAAATTCGTGATCGCGAAACCATGGAACATG
CCCTGGCCTTCGCCGACACCGGGCACCTGGTGATTTCCACCCTGCATGCTCACAATGCCAACCAGGCGCTGGACCGGGTG
ATCAATTTCTTCCCGGAAGAACGCCGGCCGCAGCTGCTCAATGATCTGGGCAACAACCTCAAGGCGTTCGTGTCGCAACG
CCTGGTGCGCACTCGCACCGGCCAGCGCCGGGCCGCGGTGGAGGTCATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GCCGCAATGAGTTCGGCGAGCTCAAGGGCATCATGGAAAAGTCAGAAGAACTCGGGATGCAAACCTTCGATCACGCCCTG
TACAACCTGGTGGTGGAGGGTGCTATCGATGAGGAGGAAGCCCTGAAAAACGCCGATTCGGCAAACAACCTGCGGTTGCG
CCTGAAGTTGCATGCCGAGTCGGGGGCAGCGGTGCCCCCGGCCGATCCGGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1N7EMY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.901

95.43

0.543
  pilU Acinetobacter baylyi ADP1

51.685

95.699

0.495
  pilU Vibrio cholerae strain A1552

52

94.086

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.235

100

0.384
  pilT Pseudomonas aeruginosa PAK

39.244

92.473

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.953

92.473

0.36