Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   R5022_RS28680 Genome accession   NZ_CP137514
Coordinates   6111337..6111876 (+) Length   179 a.a.
NCBI ID   WP_278025408.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA0170     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6106337..6116876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5022_RS28655 (R5022_28655) yacG 6106504..6106704 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  R5022_RS28660 (R5022_28660) coaE 6106701..6107312 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  R5022_RS28665 (R5022_28665) pilD 6107309..6108181 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5022_RS28670 (R5022_28670) pilC 6108184..6109401 (-) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  R5022_RS28675 (R5022_28675) pilB 6109405..6111108 (-) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5022_RS28680 (R5022_28680) pilE 6111337..6111876 (+) 540 WP_278025408.1 pilin Machinery gene
  R5022_RS28685 (R5022_28685) - 6112081..6112638 (+) 558 WP_224754157.1 hypothetical protein -
  R5022_RS28695 (R5022_28695) nadC 6112802..6113650 (-) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R5022_RS28700 (R5022_28700) - 6113936..6116191 (+) 2256 WP_033990664.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18067.49 Da        Isoelectric Point: 6.2547

>NTDB_id=821908 R5022_RS28680 WP_278025408.1 6111337..6111876(+) (pilE) [Pseudomonas aeruginosa strain HPA0170]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASLSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=821908 R5022_RS28680 WP_278025408.1 6111337..6111876(+) (pilE) [Pseudomonas aeruginosa strain HPA0170]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCTATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.243

100

0.447
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363