Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QEP70_RS18835 Genome accession   NZ_CP123221
Coordinates   4082957..4084651 (-) Length   564 a.a.
NCBI ID   WP_010216952.1    Uniprot ID   -
Organism   Pseudomonas amygdali pv. aesculi strain 2250     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4077957..4089651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEP70_RS30260 - 4078166..4078402 (-) 237 WP_327203937.1 DUF3094 family protein -
  QEP70_RS18800 - 4078287..4078820 (-) 534 WP_005732326.1 MOSC domain-containing protein -
  QEP70_RS18805 - 4078817..4079446 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  QEP70_RS18810 - 4079588..4079839 (+) 252 Protein_3710 hypothetical protein -
  QEP70_RS18815 yacG 4080037..4080246 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  QEP70_RS18820 coaE 4080243..4080866 (-) 624 WP_005732328.1 dephospho-CoA kinase -
  QEP70_RS18825 pilD 4080863..4081735 (-) 873 WP_010216946.1 A24 family peptidase Machinery gene
  QEP70_RS18830 pilC 4081737..4082954 (-) 1218 WP_005732330.1 type II secretion system F family protein Machinery gene
  QEP70_RS18835 pilB 4082957..4084651 (-) 1695 WP_010216952.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QEP70_RS18840 - 4084879..4085307 (+) 429 WP_005732335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QEP70_RS18845 - 4086140..4086685 (-) 546 WP_010216954.1 DUF1523 family protein -
  QEP70_RS18850 - 4086805..4087803 (+) 999 WP_005732338.1 ATP-grasp domain-containing protein -
  QEP70_RS18855 - 4087858..4088997 (+) 1140 WP_010216957.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62365.69 Da        Isoelectric Point: 6.2533

>NTDB_id=820086 QEP70_RS18835 WP_010216952.1 4082957..4084651(-) (pilB) [Pseudomonas amygdali pv. aesculi strain 2250]
MTDAVLTGLAKQLVLAELLTESIAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLMDAILLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYLEALKLPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRDTLIQEGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLVISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=820086 QEP70_RS18835 WP_010216952.1 4082957..4084651(-) (pilB) [Pseudomonas amygdali pv. aesculi strain 2250]
ATGACTGATGCTGTCCTCACTGGTTTGGCCAAACAACTGGTCTTGGCCGAGCTACTTACTGAGTCCATCGCGCAACAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTCAGTTATCTGGTTCAGAACAAACTGGTCAAAAGCCTGACGCTCG
CAGAAATGGCTTCCGATCAGTTCGGCGTGCCGTTCATGGATCTCGCCAGCCTTGACAAGGAAAGCCAGCCTAAAGGTCTG
GTAAGTGAAAAGCTGGTTCGCCAGCATCACGCGCTGCCCCTGTGGCGGCGCGGCAACAAGCTATTTATCGGAATTTCAGA
TCCGACCAATCATCAGGCGGTCACCGATATTCAGTTCAGTACTGGCCTGAACACCGAAGCGATTCTGGTAGAAGATGACA
AGCTAACCACTGCCATAGATCGTTTTTTTGACAGTGATAGCGGTCTAGGTAATCTGGAAGATGTCGATCTGGGTCTGGAT
ATCGAACCGGCTGATGGCAAGGAAACTTCCCTCGCTACGCAGAATGACGCAGACGATGCACCGGTGGTGCGCTTCGTCAA
CAAAATGTTGATGGACGCTATTCTATTAGGATCGTCCGACCTGCATTTTGAGCCTTACGAAAAGATCTTCCGCGTGCGGT
TGCGTACCGACGGCATCTTGCATGAAGTCGCTAGACCACCAATTCATTTGGCGAACCGTATCGCCGCGCGCTTAAAAGTG
ATGGCCAGCCTGGATATCTCGGAACGGCGCAAACCGCAGGACGGACGCGTCAAGTTACGTGTGTCCAAAACAAAAGCCAT
CGATTTTCGTATGAACACCTTGCCCACGTTGTGGGGCGAGAAGATCGTAATGCGGATCCTCGACCCAACCAGTGCGCAGA
TGGGTATCGATGCGCTGGGCTATGAGCCTGATCAAAAGGCACTGTACCTAGAAGCGCTTAAACTGCCGCAAGGGATGATT
CTTGTGACTGGCCCTACAGGTTCAGGCAAGACTGTCTCTCTCTATACAGGCCTGAACATTCTCAACACTGTGGACATTAA
CATTTCCACCGCTGAGGACCCGGTGGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGCCAAGGACTGG
ACTTTTCCCAAGCCTTGCGCGCGTTCTTGCGTCAAGATCCCGACGTGATTATGGTCGGCGAAATACGAGATCTGGAAACC
GCCGAAATCGCCATCAAAGCCTCGCAGACCGGCCATATGGTGCTTTCCACATTGCACACCAACAGCGCGGCGGAAACGCT
GACTCGCCTGCACCATATGGGCGTTGCAGCATTCAACATTGCTACAGCAATCAACCTGATTATTGCGCAGCGACTGGCAC
GTAAGCTGTGTAGCCACTGCAAGAAAGAACTGGATATCCCTCGCGACACACTGATTCAGGAAGGCTTTCCAGAAGCCAAA
ATCGGCACTTTCAAGATATACGGCCCGGTTGGCTGCGAGCACTGCAACGGGGGTTACCGAGGCAGGGTCGGCATTTACGA
AGTGGTCAAGAAAACACCGGAACTGGAACGCATCATTATGGAAGAAGGCAACTCACTGGTGATTTCCAGACAGATGCGCA
AGGACGGTTTCAATGACCTTCGCACATCAGGGCTTTCGAAAGCCATGCAAGGCATTACCAGTCTTGAAGAAGTCAACCGA
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.672

100

0.58
  pilB Acinetobacter baylyi ADP1

56.184

100

0.564
  pilB Legionella pneumophila strain ERS1305867

55.221

100

0.553
  pilB Vibrio cholerae strain A1552

50.712

99.645

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.268

99.113

0.498
  pilB Vibrio campbellii strain DS40M4

49.117

100

0.493
  pilF Neisseria gonorrhoeae MS11

47.95

99.468

0.477
  pilF Thermus thermophilus HB27

38.693

100

0.388