Detailed information NTG0820084

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QEP70_RS18825
• Genome accession: NZ_CP123221
• Coordinates: 4080863..4081735 (-)
• Length: 290 a.a.
• NCBI ID: WP_010216946.1
• Uniprot ID: A0A0Q0CUR9
• Organism: Pseudomonas amygdali pv. aesculi strain 2250
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4075863..4086735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEP70_RS18795	-	4076771..4078069 (-)	1299	WP_005732325.1	NAD(P)/FAD-dependent oxidoreductase	-
QEP70_RS30260	-	4078166..4078402 (-)	237	WP_327203937.1	DUF3094 family protein	-
QEP70_RS18800	-	4078287..4078820 (-)	534	WP_005732326.1	MOSC domain-containing protein	-
QEP70_RS18805	-	4078817..4079446 (-)	630	WP_003430735.1	DUF1780 domain-containing protein	-
QEP70_RS18810	-	4079588..4079839 (+)	252	Protein_3710	hypothetical protein	-
QEP70_RS18815	yacG	4080037..4080246 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
QEP70_RS18820	coaE	4080243..4080866 (-)	624	WP_005732328.1	dephospho-CoA kinase	-
QEP70_RS18825	pilD	4080863..4081735 (-)	873	WP_010216946.1	A24 family peptidase	Machinery gene
QEP70_RS18830	pilC	4081737..4082954 (-)	1218	WP_005732330.1	type II secretion system F family protein	Machinery gene
QEP70_RS18835	pilB	4082957..4084651 (-)	1695	WP_010216952.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QEP70_RS18840	-	4084879..4085307 (+)	429	WP_005732335.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
QEP70_RS18845	-	4086140..4086685 (-)	546	WP_010216954.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32108.25 Da
• Isoelectric Point: 8.5797

>NTDB_id=820084 QEP70_RS18825 WP_010216946.1 4080863..4081735(-) (pilD) [Pseudomonas amygdali pv. aesculi strain 2250]
MPLLDFLASSPLAFVTTCCILGLIIGSFLNVVVYRLPKMMERDWKAQSREMLGLPAEPDQPAFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQTAAMLVLSWGLLTMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=820084 QEP70_RS18825 WP_010216946.1 4080863..4081735(-) (pilD) [Pseudomonas amygdali pv. aesculi strain 2250]
ATGCCCCTCCTCGATTTCCTGGCCAGCTCCCCTCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTCCCCAAAATGATGGAGCGCGACTGGAAAGCCCAATCCCGCGAAATGCTCGGCC
TGCCCGCGGAACCCGATCAGCCCGCCTTCAACCTGAACCGCCCTCGTTCCAGCTGCCCGCACTGCGCGCACAAAATCCGC
CCGTGGGAAAACCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGTAAATGCTCACAATGCAAAGCCCCCATCAGCAAACG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGTTTCGGCTGGCAAACGGCCG
CGATGCTGGTGTTGAGCTGGGGCCTGCTGACGATGAGTCTGATCGACGCCGATCACCAGTTACTCCCTGACTCTCTGGTA
CTGCCACTGCTGTGGCTAGGCCTCATCGTCAACGCTTTCGGGCTGTTCACCTCGCTGAACGATGCGCTTTGGGGTGCGGT
GGCCGGTTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCATGGGGCGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCATCGCTGGTGGGC
GCGGTGCTTGGGGTGATAATGATGCGCGTGCGCAGGGTTGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: A0A0Q0CUR9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.986	95.172	0.514
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	51.056	97.931	0.5
pilD	Acinetobacter nosocomialis M2	50	97.931	0.49
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476