Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QEP70_RS00430 Genome accession   NZ_CP123221
Coordinates   95063..96232 (-) Length   389 a.a.
NCBI ID   WP_005732021.1    Uniprot ID   A0A108V2M6
Organism   Pseudomonas amygdali pv. aesculi strain 2250     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 90063..101232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEP70_RS00415 yejB 91347..92411 (+) 1065 WP_010215968.1 microcin C ABC transporter permease YejB -
  QEP70_RS00420 - 92411..93433 (+) 1023 WP_005732025.1 ABC transporter permease -
  QEP70_RS00425 - 93435..95012 (+) 1578 WP_010206223.1 ABC transporter ATP-binding protein -
  QEP70_RS00430 pilU 95063..96232 (-) 1170 WP_005732021.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QEP70_RS00435 - 96356..96631 (+) 276 WP_005732020.1 peptidylprolyl isomerase -
  QEP70_RS00440 pgm 96706..98352 (-) 1647 WP_005732019.1 phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) -
  QEP70_RS00445 - 98468..100795 (-) 2328 WP_010206225.1 ATP-dependent DNA helicase -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 42650.81 Da        Isoelectric Point: 5.8676

>NTDB_id=820064 QEP70_RS00430 WP_005732021.1 95063..96232(-) (pilU) [Pseudomonas amygdali pv. aesculi strain 2250]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLPGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=820064 QEP70_RS00430 WP_005732021.1 95063..96232(-) (pilU) [Pseudomonas amygdali pv. aesculi strain 2250]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGTTGGGCAGTGAAACCCTCAAACCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGCCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A108V2M6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.072

99.486

0.558
  pilU Acinetobacter baylyi ADP1

51.541

91.774

0.473
  pilU Vibrio cholerae strain A1552

51.714

89.974

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.687

98.458

0.391
  pilT Pseudomonas aeruginosa PAK

41.399

88.175

0.365