Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   R3J35_RS10460 Genome accession   NZ_CP136972
Coordinates   2279697..2280143 (+) Length   148 a.a.
NCBI ID   WP_027700144.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8173     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2274697..2285143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J35_RS10440 (R3J35_10430) - 2274945..2276282 (+) 1338 WP_027700147.1 HAMP domain-containing sensor histidine kinase -
  R3J35_RS10445 (R3J35_10435) coaE 2276549..2277172 (-) 624 WP_027700146.1 dephospho-CoA kinase -
  R3J35_RS10450 (R3J35_10440) pilD 2277184..2278047 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  R3J35_RS10455 (R3J35_10445) pilC 2278054..2279256 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  R3J35_RS10460 (R3J35_10450) pilA/pilAI 2279697..2280143 (+) 447 WP_027700144.1 pilin Machinery gene
  R3J35_RS10465 (R3J35_10455) - 2280388..2280615 (-) 228 WP_012338102.1 hypothetical protein -
  R3J35_RS10470 (R3J35_10460) pilA/pilAI 2281448..2281888 (+) 441 WP_012338104.1 pilin Machinery gene
  R3J35_RS10475 (R3J35_10465) pilB 2282728..2284461 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15379.55 Da        Isoelectric Point: 8.4988

>NTDB_id=816967 R3J35_RS10460 WP_027700144.1 2279697..2280143(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKSTSNASDIGLRTDTTRCGI
TVKIDAAGTANITCKVKGNSQVNDKTIAWDRTSDNSAGTNGVNNGGVWTCSSTVTSDALRPSGCMAAK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=816967 R3J35_RS10460 WP_027700144.1 2279697..2280143(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGAGTACATCCAACGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGAATT
ACCGTTAAAATTGATGCCGCTGGCACTGCCAACATCACATGCAAAGTTAAGGGCAACTCTCAAGTCAATGATAAAACCAT
CGCTTGGGATCGTACCTCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCCTCCACCG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATGGCTGCTAAATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.857

99.324

0.426
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.177

99.324

0.419
  pilA Vibrio cholerae strain A1552

38.71

100

0.405
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.71

100

0.405
  pilA Vibrio cholerae C6706

38.71

100

0.405
  pilA Acinetobacter baumannii strain A118

40

100

0.405