Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HSX14_RS28165 Genome accession   NZ_AP023189
Coordinates   6145399..6147477 (-) Length   692 a.a.
NCBI ID   WP_173171172.1    Uniprot ID   A0A6J4EDM8
Organism   Pseudomonas tohonis strain TUM18999     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6140399..6152477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSX14_RS28150 (TUM18999_56070) - 6142036..6143709 (-) 1674 WP_173171178.1 AAA family ATPase -
  HSX14_RS28155 (TUM18999_56080) aroB 6143720..6144823 (-) 1104 WP_173171176.1 3-dehydroquinate synthase -
  HSX14_RS28160 (TUM18999_56090) aroK 6144875..6145393 (-) 519 WP_173171174.1 shikimate kinase AroK -
  HSX14_RS28165 (TUM18999_56100) pilQ 6145399..6147477 (-) 2079 WP_173171172.1 type IV pilus secretin PilQ Machinery gene
  HSX14_RS28170 (TUM18999_56110) pilP 6147531..6148064 (-) 534 WP_173171170.1 type 4a pilus biogenesis lipoprotein PilP -
  HSX14_RS28175 (TUM18999_56120) pilO 6148061..6148684 (-) 624 WP_173171168.1 type 4a pilus biogenesis protein PilO -
  HSX14_RS28180 (TUM18999_56130) - 6148681..6149253 (-) 573 WP_173171166.1 PilN domain-containing protein -
  HSX14_RS28185 (TUM18999_56140) pilM 6149253..6150317 (-) 1065 WP_173171164.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74170.19 Da        Isoelectric Point: 4.8779

>NTDB_id=81593 HSX14_RS28165 WP_173171172.1 6145399..6147477(-) (pilQ) [Pseudomonas tohonis strain TUM18999]
MNSSLSRFGLTLLAAMLSPVLLAADLQGLDVAALPGGKVELKLAFDEPVVAPRGYTIEQPARIALDLPGVTNKLGSKNRE
LGVGNARSVTIVEAKDRTRMIINLATLVPYSTRVEGNNLYVVVGEAGGATQQATRTSPVAAPAAPAAPVKTFAAAGKAIS
NIDFQRGDQGEGNVVITLSDPTVSPDIQEQGGKIRLDFAKTQLPESLRVRLDVKDFATPVQFVSAAGSSGNASITIEPTG
FYDYLAYQTDNKLTVSIKPLTQEDVEKRKSERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVTSGNE
SSDERGSITVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVNWRGARVGDNNFVIGGEG
GTRTGGGGTSPNAALGTFVDMGATGATSSIGIGFVTDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGAEIPY
QEASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFSNALNGVPPINKNEVNAKVLVSDGETIVIGGVFSN
TQTKATDKVPFLGDLPFLGRMFKRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=81593 HSX14_RS28165 WP_173171172.1 6145399..6147477(-) (pilQ) [Pseudomonas tohonis strain TUM18999]
ATGAACAGCTCGCTTTCGCGCTTCGGCCTTACCCTATTGGCCGCCATGTTGTCGCCAGTATTGCTGGCTGCCGACCTGCA
GGGTCTCGATGTGGCAGCCTTGCCTGGCGGTAAGGTTGAACTGAAACTTGCCTTCGACGAGCCGGTTGTCGCGCCTCGGG
GCTATACCATCGAGCAGCCTGCGCGTATTGCCTTGGACCTGCCCGGGGTAACCAACAAGCTGGGCAGCAAGAATCGTGAG
CTGGGTGTGGGCAACGCGCGGAGCGTCACCATAGTCGAGGCCAAGGATCGCACCCGGATGATTATCAACCTGGCGACACT
CGTGCCCTACAGCACGCGTGTCGAGGGTAACAATCTCTATGTCGTGGTGGGTGAGGCGGGCGGTGCCACGCAGCAAGCGA
CCCGTACTTCTCCTGTTGCCGCTCCGGCAGCCCCCGCAGCCCCCGTCAAGACTTTTGCTGCGGCAGGCAAGGCCATCAGC
AACATTGATTTTCAGCGCGGTGACCAGGGTGAAGGCAATGTTGTAATCACGCTTTCCGATCCCACTGTAAGCCCGGATAT
TCAGGAACAGGGTGGCAAGATTCGCCTTGATTTCGCCAAGACCCAATTGCCCGAGTCGTTGCGCGTACGCCTGGATGTGA
AGGATTTCGCTACGCCGGTTCAGTTCGTCAGCGCAGCGGGCTCTTCAGGTAACGCCAGCATCACCATCGAGCCGACCGGC
TTCTACGATTACCTGGCCTATCAAACCGATAACAAGTTGACTGTCAGCATCAAGCCGCTGACGCAAGAAGATGTCGAGAA
GCGCAAGTCCGAGCGTTTTGCCTACTCGGGTGAAAAGCTCTCTTTGAACTTCCAGGACATCGATGTGCGTTCGGTGCTTC
AGCTGATTGCTGACTTCACCGACCTCAACCTGGTGGCCTCGGACACCGTGCAGGGCAACATCACCCTGCGCCTGCAGAAC
GTGCCCTGGGACCAGGCGTTGGACCTGGTGCTGAAAACCAAGGGGCTCGACAAGCGCAAGGTTGGCAACGTGCTGCTGGT
TGCACCGGCAGACGAAATTGCTGCTCGTGAGCGTCAAGAGCTGGAGTCCCAGAAGCAGATCGCCGAGTTGGCTCCGCTGC
GTCGTGAGCTGATCCAGGTGAACTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCCGTGACCAGTGGCAACGAG
TCGTCGGATGAGCGTGGTTCGATCACTGTGGATGACCGCACCAACAGCATCATCGCCTACCAGACCCAGGACAAGCTGGA
CGAACTGCGCCGCATCGTGGCCCAGCTCGATATCCCGGTCCGTCAGGTGATGATCGAGGCTCGTATCGTCGAGGCGAACG
TCGACTACGACAAATCGCTGGGTGTGAACTGGCGCGGTGCGCGTGTGGGTGACAACAACTTCGTCATCGGCGGGGAAGGT
GGCACTCGTACCGGTGGTGGTGGCACCAGCCCCAACGCGGCTCTCGGTACCTTTGTTGATATGGGTGCTACAGGTGCTAC
TTCCTCCATTGGCATTGGGTTCGTAACCGACAACACCATCCTCGACCTCGAACTCTCCGCCATGGAGAAGACCGGCAACG
GCGAGATCGTATCCCAGCCGAAGGTCGTAACTTCGGACAAGGAAACCGCGAAGATCCTTAAGGGCGCCGAGATTCCCTAT
CAGGAAGCCAGCTCCAGCGGTGCCAGTACCACATCCTTCAAGGAAGCGGCCCTGTCCCTTGAAGTGACTCCGCAGATCAC
GCCTGACAACCGCATCATCATGGAAGTCAAGGTAACCAAGGATGCCCCGGACTTCTCCAACGCGCTGAACGGCGTACCGC
CGATCAACAAAAACGAGGTCAATGCCAAGGTTCTGGTCAGCGATGGCGAGACCATCGTGATCGGCGGTGTGTTCTCCAAC
ACCCAGACCAAGGCGACCGACAAGGTTCCCTTCCTCGGCGACCTGCCGTTCCTCGGCCGCATGTTCAAGCGTGATGTGGT
CCAGGACAAGAAATCCGAGCTGCTGGTCTTCCTCACCCCGCGTATCATGAACAACCAGGCTATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(40-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6J4EDM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.483

100

0.801
  comQ Acinetobacter baylyi ADP1

42.919

100

0.429
  pilQ Acinetobacter baumannii strain A118

39.756

100

0.423
  pilQ Acinetobacter baumannii D1279779

39.756

100

0.423
  pilQ Legionella pneumophila strain Lp02

38.336

97.254

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.187

97.254

0.371

Multiple sequence alignment