Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HSX14_RS28185 Genome accession   NZ_AP023189
Coordinates   6149253..6150317 (-) Length   354 a.a.
NCBI ID   WP_173171164.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain TUM18999     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6144253..6155317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSX14_RS28160 (TUM18999_56090) aroK 6144875..6145393 (-) 519 WP_173171174.1 shikimate kinase AroK -
  HSX14_RS28165 (TUM18999_56100) pilQ 6145399..6147477 (-) 2079 WP_173171172.1 type IV pilus secretin PilQ Machinery gene
  HSX14_RS28170 (TUM18999_56110) pilP 6147531..6148064 (-) 534 WP_173171170.1 type 4a pilus biogenesis lipoprotein PilP -
  HSX14_RS28175 (TUM18999_56120) pilO 6148061..6148684 (-) 624 WP_173171168.1 type 4a pilus biogenesis protein PilO -
  HSX14_RS28180 (TUM18999_56130) - 6148681..6149253 (-) 573 WP_173171166.1 PilN domain-containing protein -
  HSX14_RS28185 (TUM18999_56140) pilM 6149253..6150317 (-) 1065 WP_173171164.1 pilus assembly protein PilM Machinery gene
  HSX14_RS28190 (TUM18999_56150) - 6150502..6152922 (+) 2421 WP_173171700.1 penicillin-binding protein 1A -
  HSX14_RS28195 (TUM18999_56160) - 6153036..6154304 (-) 1269 WP_173171162.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37680.91 Da        Isoelectric Point: 4.4429

>NTDB_id=81594 HSX14_RS28185 WP_173171164.1 6149253..6150317(-) (pilM) [Pseudomonas tohonis strain TUM18999]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLGKAKASSKSVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAAQLGNGSDELTVAVVDIGATMTTLSVLHNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTQVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=81594 HSX14_RS28185 WP_173171164.1 6149253..6150317(-) (pilM) [Pseudomonas tohonis strain TUM18999]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGCTCGACTTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCACCGAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGTTGTCCCGTGTTCTTGGCAAGGCCAAGGCCAGTTCGAAGTCCGTCGCCGTA
GCGGTCGCGGGCTCCGCTGTAATCACCAAGACGATCGAGATGGATGCCGGGCTTTCCGACGATGAGTTGGAGAACCAGCT
GAAGATCGAGGCGGACCAGTACATCCCTTACCCGCTCGAAGAGGTCGCAATCGACTTCGAGGTCCAGGGTGCTTCCGCGC
GCAATCCTGAGCGGGTCGATGTGCTTCTCGCCGCGTGCCGCAAGGAGAACGTCGAGGTCCGTGAAGCCGCTCTGGCCCTG
GCGGGCTTGACCGCCAAGGTCGTAGACGTCGAAGCCTATGCGCTGGAACGTGCCTACGGACTTCTCGCTGCGCAACTGGG
TAACGGGAGTGACGAACTGACGGTCGCCGTCGTGGATATCGGGGCGACCATGACCACCCTGAGCGTCCTGCACAACGGCC
GGACCATCTACAGCCGCGAGCAGCTTTTCGGCGGGCGCCAGCTCACCGAGGAGATCCAGCGTCGCTATGGCCTGTCCGTC
GAAGAAGCGGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGTGAAGTGTTGCAGCCGTTCAAAGACGC
AGTGGTCCAGCAGGTGTCTCGTTCGCTGCAGTTCTTCTTCGCTGCGGGCCAGTTCAACGATGTCGACTACATCCTCCTGG
CGGGTGGTACGGCGTCCATTCCTGATCTGGATCGTTTGATCCAGCAGAAGATCGGCACTCCTACCCAGGTTGCCAACCCC
TTCGCGGAGATGGCGCTGAGTGGCAAGGTCAATGCGGGCGCATTGGCCAGTGATGCCCCGGCGCTGATGATTGCCTGCGG
ACTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

47.74

100

0.477

Multiple sequence alignment