Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   M0M55_RS23730 Genome accession   NZ_CP136800
Coordinates   5103090..5103554 (-) Length   154 a.a.
NCBI ID   WP_021205141.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain A6-17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5098090..5108554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0M55_RS23715 (M0M55_23715) nadC 5100601..5101449 (+) 849 WP_021264134.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M0M55_RS23725 (M0M55_23725) - 5101629..5103008 (-) 1380 WP_231280141.1 O-antigen ligase family protein -
  M0M55_RS23730 (M0M55_23730) pilA/pilAI 5103090..5103554 (-) 465 WP_021205141.1 pilin Machinery gene
  M0M55_RS23735 (M0M55_23735) pilB 5103785..5105485 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0M55_RS23740 (M0M55_23740) pilC 5105489..5106706 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  M0M55_RS23745 (M0M55_23745) pilD 5106707..5107579 (+) 873 WP_108083860.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  M0M55_RS23750 (M0M55_23750) coaE 5107576..5108187 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  M0M55_RS23755 (M0M55_23755) yacG 5108184..5108384 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16076.44 Da        Isoelectric Point: 9.0145

>NTDB_id=815773 M0M55_RS23730 WP_021205141.1 5103090..5103554(-) (pilA/pilAI) [Pseudomonas aeruginosa strain A6-17]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPTKDQYDIGFTG
STLLKGSEKGQITVKNGTLPTVQLEATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPANWKPNYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=815773 M0M55_RS23730 WP_021205141.1 5103090..5103554(-) (pilA/pilAI) [Pseudomonas aeruginosa strain A6-17]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGACTCCTACCAAGGACCAATATGATATTGGATTTACCGGT
TCTACCTTGCTGAAAGGGTCGGAGAAAGGTCAGATTACTGTGAAAAATGGTACTCTGCCTACTGTGCAGTTGGAAGCCAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTTTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTGCTAACTGGAAACCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.945

94.805

0.455
  pilA Pseudomonas aeruginosa PAK

42.857

100

0.448
  pilA Acinetobacter baumannii strain A118

45.205

94.805

0.429
  comP Acinetobacter baylyi ADP1

39.264

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.776

95.455

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

90.26

0.37