Detailed information NTG0081575

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: HSX14_RS05955
• Genome accession: NZ_AP023189
• Coordinates: 1283950..1285281 (+)
• Length: 443 a.a.
• NCBI ID: WP_173173370.1
• Uniprot ID: A0A6J4E1Y0
• Organism: Pseudomonas tohonis strain TUM18999
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 1278950..1290281

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HSX14_RS05930 (TUM18999_11800)	pgeF	1279084..1279809 (-)	726	WP_173173378.1	peptidoglycan editing factor PgeF	-
HSX14_RS05935 (TUM18999_11810)	rluD	1279809..1280780 (-)	972	WP_373874679.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
HSX14_RS05940 (TUM18999_11820)	-	1280929..1281948 (+)	1020	WP_173173376.1	outer membrane protein assembly factor BamD	-
HSX14_RS05945 (TUM18999_11830)	-	1282129..1282365 (+)	237	WP_173173374.1	PP0621 family protein	-
HSX14_RS05950 (TUM18999_11840)	pilS	1282355..1283947 (+)	1593	WP_228723545.1	sensor histidine kinase	Regulator
HSX14_RS05955 (TUM18999_11850)	pilR	1283950..1285281 (+)	1332	WP_173173370.1	sigma-54-dependent transcriptional regulator	Regulator
HSX14_RS05960 (TUM18999_11860)	thiO	1285294..1286391 (-)	1098	WP_373874678.1	glycine oxidase ThiO	-
HSX14_RS05965 (TUM18999_11870)	-	1286420..1286947 (+)	528	WP_173173368.1	GspH/FimT family pseudopilin	-
HSX14_RS05970 (TUM18999_11880)	-	1287059..1287574 (+)	516	WP_173173599.1	GspH/FimT family pseudopilin	-
HSX14_RS05975 (TUM18999_11890)	pilV	1287561..1288136 (+)	576	WP_173173366.1	type IV pilus modification protein PilV	-
HSX14_RS05980 (TUM18999_11900)	-	1288133..1288936 (+)	804	WP_173173364.1	PilW family protein	-
HSX14_RS05985 (TUM18999_11910)	-	1288948..1289493 (+)	546	WP_173173362.1	pilus assembly PilX family protein	-
HSX14_RS05990 (TUM18999_11920)	-	1289506..1289847 (+)	342	WP_173173360.1	hypothetical protein	-

Sequence

Protein

• Length: 443 a.a.
• Molecular weight: 49441.66 Da
• Isoelectric Point: 5.5835

>NTDB_id=81575 HSX14_RS05955 WP_173173370.1 1283950..1285281(+) (pilR) [Pseudomonas tohonis strain TUM18999]
MTRQRALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAAEPFDLCLTDMRLPDGTGLDLVQHIQQRHPQVPVAM
ITAYGSLDTAVNALKAGAFDFLTKPVDLGRLRELVAAALRLRTSGSGDTPMDNRLLGDSPPMKALRNQIQKLARSQAPVY
ISGESGSGKELVARMIHEQGPRHEDPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKIGLFQAAHGGTLFLDEVADLP
LPMQVKLLRAIQEKAIRTIGGQQEVVVDVRILSATHKDLAAEVAAGRFRQDLYYRLNVIELSVPPLRERREDIPQLADVM
LKRLAADSAAASITRDAMEKLKSYRFPGNVRELENMLERAYTLCDNDQIQPEDLRLADAPSPSESSEADLAQVSNLEDYL
EEIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1332 bp

>NTDB_id=81575 HSX14_RS05955 WP_173173370.1 1283950..1285281(+) (pilR) [Pseudomonas tohonis strain TUM18999]
ATGACCAGACAGAGAGCACTCATCGTCGACGACGAGCCCGATATCCGCGAGCTGCTGGAGATCACCCTCGGCCGGATGAA
GCTGGATACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGTGAACTCCTCGCCGCCGAACCCTTCGACCTGTGCCTGACCG
ACATGCGCCTGCCGGACGGCACCGGCCTCGACCTCGTGCAGCACATCCAGCAGCGCCATCCGCAGGTCCCGGTGGCGATG
ATCACCGCCTACGGCAGCCTGGACACCGCCGTCAACGCACTGAAGGCCGGCGCCTTCGACTTCCTCACCAAGCCTGTGGA
CCTCGGCCGGCTTCGCGAGCTGGTCGCCGCGGCGCTGCGCCTGCGCACCAGCGGCAGCGGCGACACGCCGATGGACAACC
GCCTGCTCGGCGACTCGCCGCCGATGAAGGCGCTGCGCAACCAGATCCAGAAGCTCGCGCGCAGCCAGGCCCCCGTCTAC
ATCAGCGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCACGCATGATCCACGAGCAGGGCCCGCGCCACGAAGACCCCTT
CGTACCGGTCAACTGCGGCGCCATCCCCTCGGAACTGATGGAAAGCGAATTCTTCGGCCACAAGAAAGGCAGCTTCACCG
GCGCCATCGAAGACAAGATCGGCCTGTTCCAGGCGGCCCATGGCGGCACCCTGTTCCTGGACGAAGTGGCAGACCTGCCC
CTGCCCATGCAGGTGAAACTGCTGCGTGCCATCCAGGAAAAGGCCATTCGCACCATAGGTGGCCAGCAGGAAGTGGTGGT
GGACGTACGCATTCTCAGCGCCACCCACAAGGACCTGGCTGCCGAAGTGGCGGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTGAACGTCATCGAACTCAGCGTCCCTCCGCTGCGCGAGCGCCGCGAGGACATCCCGCAACTGGCCGATGTGATG
CTCAAGCGCCTGGCTGCGGACAGCGCCGCGGCCAGCATTACCCGCGACGCCATGGAAAAGCTCAAGAGCTATCGCTTCCC
CGGCAACGTCCGCGAGCTGGAAAACATGCTCGAGCGGGCCTACACGCTCTGCGACAACGACCAGATCCAGCCCGAGGACC
TGCGCCTGGCCGACGCGCCCTCGCCGTCGGAAAGCAGCGAGGCCGATCTAGCGCAGGTCAGCAACCTCGAGGATTACCTG
GAAGAGATCGAGCGCAAGCTCATCATGCAGGCCCTAGAAGAAACCCGCTGGAACCGCACCGCCGCAGCCCAGCGCCTGGG
CCTGACCTTCCGCTCGATGCGCTACCGCCTGAAGAAACTGGGGATCGACTGA

Domains

Predicted by InterproScan.

• Sigma-54 interaction domain (Sigma54_activat) (136-301)

• Response regulator receiver domain (Response_reg) (7-115)

• Bacterial regulatory protein, Fis family (HTH_8) (400-440)

3D structure

• AlphaFold DB: A0A6J4E1Y0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	85.843	100	0.862
pilR	Acinetobacter baumannii strain A118	49.892	100	0.519
luxO	Vibrio cholerae strain A1552	37.557	99.774	0.375