Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   HSX14_RS05950 Genome accession   NZ_AP023189
Coordinates   1282355..1283947 (+) Length   530 a.a.
NCBI ID   WP_228723545.1    Uniprot ID   A0A6J4DZB8
Organism   Pseudomonas tohonis strain TUM18999     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1277355..1288947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSX14_RS05930 (TUM18999_11800) pgeF 1279084..1279809 (-) 726 WP_173173378.1 peptidoglycan editing factor PgeF -
  HSX14_RS05935 (TUM18999_11810) rluD 1279809..1280780 (-) 972 WP_373874679.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  HSX14_RS05940 (TUM18999_11820) - 1280929..1281948 (+) 1020 WP_173173376.1 outer membrane protein assembly factor BamD -
  HSX14_RS05945 (TUM18999_11830) - 1282129..1282365 (+) 237 WP_173173374.1 PP0621 family protein -
  HSX14_RS05950 (TUM18999_11840) pilS 1282355..1283947 (+) 1593 WP_228723545.1 sensor histidine kinase Regulator
  HSX14_RS05955 (TUM18999_11850) pilR 1283950..1285281 (+) 1332 WP_173173370.1 sigma-54-dependent transcriptional regulator Regulator
  HSX14_RS05960 (TUM18999_11860) thiO 1285294..1286391 (-) 1098 WP_373874678.1 glycine oxidase ThiO -
  HSX14_RS05965 (TUM18999_11870) - 1286420..1286947 (+) 528 WP_173173368.1 GspH/FimT family pseudopilin -
  HSX14_RS05970 (TUM18999_11880) - 1287059..1287574 (+) 516 WP_173173599.1 GspH/FimT family pseudopilin -
  HSX14_RS05975 (TUM18999_11890) pilV 1287561..1288136 (+) 576 WP_173173366.1 type IV pilus modification protein PilV -
  HSX14_RS05980 (TUM18999_11900) - 1288133..1288936 (+) 804 WP_173173364.1 PilW family protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59007.36 Da        Isoelectric Point: 8.9929

>NTDB_id=81574 HSX14_RS05950 WP_228723545.1 1282355..1283947(+) (pilS) [Pseudomonas tohonis strain TUM18999]
MSGESTVLGGQPARRIFRLYNLYRLIIGLALVGLISSELDSELLDLARAQLFRNASWFYLLLNILIALMVRHPKHMLQVF
SLALVDVILLSCLFYAAGGTPSGIGNLVIVTVAIANILLRGRVGLLIAAIATIGMIYLTFYLSLSRPEVANQYVQAGALG
ALCFAAALFVQGISKRLQLSETLAEKRAADVANLEALNALILQRMRTGILVLDAQHRVLLANQGAQTLLGRDDLAGRIID
PQSPELVKRLQQWMDNPALRPQSLQAMPEGPVLQPSFVPLQRGEQRDTLIFLEDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELTGPDRRLAQIIQDQSRRMNLVIENVLQLSRRRQSEPQLLDLKYWLHRFASEFRNASGPG
QTLHIETNSGTIQTRMDPHQLTQVLTNLVQNGLRYSFQKNKVGQVWLRLFRDPESDLPILEIMDDGAGVPPEQVQHIFEP
FYTTESKGTGLGLYISRELCESNQARLDYRPRETGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=81574 HSX14_RS05950 WP_228723545.1 1282355..1283947(+) (pilS) [Pseudomonas tohonis strain TUM18999]
GTGAGCGGTGAGTCGACGGTCCTGGGCGGGCAGCCGGCGCGGCGCATCTTCCGCCTCTACAACCTCTACCGGCTGATCAT
CGGCCTCGCGCTGGTCGGGCTGATCTCCAGCGAGCTGGACAGCGAACTGCTCGACCTCGCCCGCGCGCAACTGTTCCGCA
ACGCCTCCTGGTTCTACCTGCTGCTGAACATCCTGATCGCCCTGATGGTCCGCCATCCGAAGCACATGCTGCAGGTGTTC
AGCCTGGCACTCGTGGACGTGATCCTGCTGTCCTGCCTGTTCTACGCGGCCGGCGGTACGCCCAGCGGCATCGGCAACCT
GGTGATAGTCACGGTGGCCATCGCCAACATCCTGCTGCGGGGCCGGGTCGGCCTGCTGATTGCCGCCATCGCCACCATCG
GCATGATCTACCTGACCTTCTACCTCAGCCTCAGCCGCCCGGAAGTCGCCAACCAGTACGTACAGGCCGGCGCGCTCGGC
GCCCTCTGTTTTGCGGCCGCCCTCTTCGTCCAGGGCATCAGCAAACGCCTGCAACTGAGCGAGACCCTGGCGGAAAAGCG
CGCCGCCGACGTCGCCAACCTCGAGGCACTCAACGCCCTGATCCTGCAACGGATGCGCACCGGCATCCTGGTGCTCGACG
CGCAACACCGCGTGCTCCTGGCCAACCAGGGCGCCCAGACCCTGCTGGGCCGCGATGACCTCGCCGGACGCATCATCGAC
CCGCAGAGCCCGGAACTGGTCAAGCGCCTCCAGCAGTGGATGGACAACCCGGCCCTGCGCCCGCAGAGCCTGCAGGCCAT
GCCCGAAGGCCCGGTGCTGCAACCCAGCTTCGTACCGCTGCAACGGGGCGAGCAACGCGACACCCTGATCTTCCTCGAAG
ACATCTCGCAGATCGCCCAGCAGGCGCAACAGCTCAAGCTCGCCTCCCTCGGCCGTCTGACCGCCGGCATCGCCCATGAG
ATACGCAACCCCCTGGGCGCCATCAGCCATGCCGCGCAGCTGCTGCAGGAGTCGGAAGAGCTGACCGGCCCAGACCGACG
CCTGGCGCAGATCATCCAGGACCAGTCGCGACGCATGAACCTGGTGATCGAGAACGTCCTGCAGCTGTCACGCCGCCGCC
AGTCAGAACCCCAACTGCTCGACCTGAAGTACTGGCTGCACCGCTTCGCCAGCGAATTCCGCAATGCTTCCGGTCCCGGC
CAGACACTGCACATCGAGACCAACAGCGGCACGATCCAGACCCGCATGGACCCGCACCAGCTGACCCAGGTACTGACCAA
CCTGGTACAGAACGGTCTGCGCTACAGTTTTCAGAAGAACAAGGTCGGACAGGTCTGGCTTCGCCTATTCCGTGATCCGG
AAAGCGACCTGCCCATCCTGGAGATCATGGATGATGGAGCCGGCGTGCCGCCCGAGCAGGTGCAGCACATCTTCGAACCC
TTCTACACGACTGAAAGCAAGGGTACCGGGCTCGGCCTGTACATCTCGCGCGAGCTGTGCGAGAGCAACCAGGCCCGCCT
CGACTACCGCCCACGCGAAACCGGCGGCAGCTGTTTTCGCATTACCTTTGCCCACCCGCGCAAATTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6J4DZB8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

65.849

100

0.658

Multiple sequence alignment