Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS25_RS04480 Genome accession   NZ_CP121859
Coordinates   918748..920454 (+) Length   568 a.a.
NCBI ID   WP_139411606.1    Uniprot ID   -
Organism   Aeromonas veronii strain LOID 15995     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 913748..925454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS25_RS04455 pdhR 914908..915672 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QBS25_RS04460 ampD 915951..916526 (-) 576 WP_069554218.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS25_RS04465 - 916657..917127 (+) 471 WP_274160611.1 retropepsin-like aspartic protease -
  QBS25_RS04470 nadC 917131..917994 (+) 864 WP_268011569.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS25_RS04475 - 918310..918744 (+) 435 WP_274160612.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS25_RS04480 pilB 918748..920454 (+) 1707 WP_139411606.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS25_RS04485 pilC 920695..921936 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  QBS25_RS04490 pilD 921998..922870 (+) 873 WP_274160613.1 A24 family peptidase Machinery gene
  QBS25_RS04495 coaE 922884..923498 (+) 615 WP_201947199.1 dephospho-CoA kinase -
  QBS25_RS04500 zapD 923536..924258 (+) 723 WP_005336141.1 cell division protein ZapD -
  QBS25_RS04505 yacG 924268..924462 (+) 195 WP_120416066.1 DNA gyrase inhibitor YacG -
  QBS25_RS04510 mutT 924540..924944 (-) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62560.90 Da        Isoelectric Point: 5.3192

>NTDB_id=814846 QBS25_RS04480 WP_139411606.1 918748..920454(+) (pilB) [Aeromonas veronii strain LOID 15995]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENNRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814846 QBS25_RS04480 WP_139411606.1 918748..920454(+) (pilB) [Aeromonas veronii strain LOID 15995]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAGCACCACGTCCTCCCTATCTACACCCAGGGCCACACCCTATACATCGCCATGTCGGA
TCCGACCAATGTCTCGGCATTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTGGAGGAGAATA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAAATT
TCGGATCTCGAGGTCTCCGACGAGAACAACCGGCTGGATGAAAGCGTCAATACCGCCGATGACGATGCACCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGTGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTCCGCTTTCGCATCGACGGCATTCTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCTTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCGCAG
GGGATGATACTGGTCACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCTTGCGCTCCTTCCTGCGGCAAGATCCGGATGTGGTGATGGTGGGGGAAATACGGGAT
CTGGAAACTGCCGAAATCGCCATCAAGGCGGCCCAGACCGGCCACTTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
TGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTCACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGCGCAAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAAGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377