Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS18_RS01145 Genome accession   NZ_CP121856
Coordinates   223048..224754 (-) Length   568 a.a.
NCBI ID   WP_139411606.1    Uniprot ID   -
Organism   Aeromonas veronii strain LOID 16013     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218048..229754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS18_RS01115 mutT 218558..218962 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  QBS18_RS01120 yacG 219040..219234 (-) 195 WP_120416066.1 DNA gyrase inhibitor YacG -
  QBS18_RS01125 zapD 219244..219966 (-) 723 WP_005336141.1 cell division protein ZapD -
  QBS18_RS01130 coaE 220004..220618 (-) 615 WP_201947199.1 dephospho-CoA kinase -
  QBS18_RS01135 pilD 220632..221504 (-) 873 WP_274160613.1 A24 family peptidase Machinery gene
  QBS18_RS01140 pilC 221566..222807 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  QBS18_RS01145 pilB 223048..224754 (-) 1707 WP_139411606.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS18_RS01150 - 224758..225192 (-) 435 WP_274160612.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS18_RS01155 nadC 225508..226371 (-) 864 WP_268011569.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS18_RS01160 - 226375..226845 (-) 471 WP_274160611.1 retropepsin-like aspartic protease -
  QBS18_RS01165 ampD 226976..227551 (+) 576 WP_069554218.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS18_RS01170 pdhR 227830..228594 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62560.90 Da        Isoelectric Point: 5.3192

>NTDB_id=814816 QBS18_RS01145 WP_139411606.1 223048..224754(-) (pilB) [Aeromonas veronii strain LOID 16013]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENNRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814816 QBS18_RS01145 WP_139411606.1 223048..224754(-) (pilB) [Aeromonas veronii strain LOID 16013]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAGCACCACGTCCTCCCTATCTACACCCAGGGCCACACCCTATACATCGCCATGTCGGA
TCCGACCAATGTCTCGGCATTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTGGAGGAGAATA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAAATT
TCGGATCTCGAGGTCTCCGACGAGAACAACCGGCTGGATGAAAGCGTCAATACCGCCGATGACGATGCACCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGTGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTCCGCTTTCGCATCGACGGCATTCTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCTTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCGCAG
GGGATGATACTGGTCACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCTTGCGCTCCTTCCTGCGGCAAGATCCGGATGTGGTGATGGTGGGGGAAATACGGGAT
CTGGAAACTGCCGAAATCGCCATCAAGGCGGCCCAGACCGGCCACTTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
TGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTCACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGCGCAAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAAGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377