Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS20_RS01775 Genome accession   NZ_CP121843
Coordinates   356457..358163 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 16549     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351457..363163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS20_RS01745 mutT 351968..352372 (+) 405 WP_107684321.1 8-oxo-dGTP diphosphatase MutT -
  QBS20_RS01750 yacG 352450..352644 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS20_RS01755 zapD 352654..353376 (-) 723 WP_005336141.1 cell division protein ZapD -
  QBS20_RS01760 coaE 353414..354028 (-) 615 WP_107684319.1 dephospho-CoA kinase -
  QBS20_RS01765 pilD 354042..354914 (-) 873 WP_107684318.1 A24 family peptidase Machinery gene
  QBS20_RS01770 pilC 354976..356217 (-) 1242 WP_005355091.1 type II secretion system F family protein Machinery gene
  QBS20_RS01775 pilB 356457..358163 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS20_RS01780 - 358167..358631 (-) 465 WP_107684404.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS20_RS01785 nadC 358960..359823 (-) 864 WP_139730941.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS20_RS01790 - 359827..360297 (-) 471 WP_139730942.1 retropepsin-like aspartic protease -
  QBS20_RS01795 ampD 360428..361003 (+) 576 WP_139730943.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS20_RS01800 pdhR 361282..362046 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=814626 QBS20_RS01775 WP_005336134.1 356457..358163(-) (pilB) [Aeromonas veronii strain ANYA 16549]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814626 QBS20_RS01775 WP_005336134.1 356457..358163(-) (pilB) [Aeromonas veronii strain ANYA 16549]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCTCAGCGCTA
TCTGAGCCAGGCCAAGGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAGCACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AACTCACCGCCGCCATCGGTAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCTGATCTCGAAGTCTCCGACGAAAACAGTCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCACCCATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTTTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTTATGGCACGGCTCGATATCGCCGAACGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAACAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACAGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTAACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTGGAAACTGCCGAAATCGCCATCAAGGCAGCACAAACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCGGCTGC
CGAAACCCTGACCCGAATGATGAACATGGGGGTACCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGCAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTACCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCAGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CGATCGCCCAGAAGGAAGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAAGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377