Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS02_RS06615 Genome accession   NZ_CP121835
Coordinates   1441826..1443532 (+) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18462     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1436826..1448532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS02_RS06590 pdhR 1437941..1438705 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QBS02_RS06595 ampD 1438984..1439559 (-) 576 WP_279506141.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS02_RS06600 - 1439690..1440160 (+) 471 WP_040068932.1 retropepsin-like aspartic protease -
  QBS02_RS06605 nadC 1440164..1441036 (+) 873 WP_279506144.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS02_RS06610 - 1441367..1441822 (+) 456 WP_201943837.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS02_RS06615 pilB 1441826..1443532 (+) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS02_RS06620 pilC 1443772..1445013 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  QBS02_RS06625 pilD 1445075..1445947 (+) 873 WP_279506146.1 A24 family peptidase Machinery gene
  QBS02_RS06630 coaE 1445961..1446575 (+) 615 WP_279506148.1 dephospho-CoA kinase -
  QBS02_RS06635 zapD 1446613..1447335 (+) 723 WP_005336141.1 cell division protein ZapD -
  QBS02_RS06640 yacG 1447345..1447539 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS02_RS06645 mutT 1447617..1448021 (-) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=814489 QBS02_RS06615 WP_005336134.1 1441826..1443532(+) (pilB) [Aeromonas veronii strain ANYA 18462]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814489 QBS02_RS06615 WP_005336134.1 1441826..1443532(+) (pilB) [Aeromonas veronii strain ANYA 18462]
ATGACCTCTAGTCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGACTTTTGCGAACTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTACCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGATGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGTCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCAATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTACGAGTTCAAGTATC
GGGTGCGTTTTCGCATCGATGGCATTCTGCATGAAATTGCTACCCCGCCGGTTAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGGCTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTACTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAGCTTGGCTTTGATGACCGCCAGAAGGCGCAGTACCTGCGCGCCCTCTCGAAACCGCAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCTGCCCAAACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTACCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCAGAGAACATCGCCAAATTGATCATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATCGCCCAAAAGGAGGGGATGCGTACCCTGCGCACCTCGGGACTAGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAAGTCAACCGGGTCACCACTAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377