Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS21_RS17480 Genome accession   NZ_CP121817
Coordinates   3712753..3713862 (-) Length   369 a.a.
NCBI ID   WP_107683852.1    Uniprot ID   A0A2T4N0R6
Organism   Aeromonas veronii strain ANYA 19170     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3707753..3718862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS21_RS17450 - 3708204..3709211 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  QBS21_RS17455 glnK 3709470..3709808 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS21_RS17460 arfB 3710010..3710426 (+) 417 WP_019445599.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS21_RS17465 - 3710459..3710950 (+) 492 WP_107683803.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS21_RS17470 - 3711312..3711737 (-) 426 WP_005340360.1 hypothetical protein -
  QBS21_RS17475 yaaA 3711932..3712705 (-) 774 WP_107683804.1 peroxide stress protein YaaA -
  QBS21_RS17480 pilU 3712753..3713862 (-) 1110 WP_107683852.1 type IVa pilus ATPase TapU Machinery gene
  QBS21_RS17485 pilT 3713891..3714925 (-) 1035 WP_107683805.1 type IVa pilus ATPase TapT Machinery gene
  QBS21_RS17490 - 3714965..3715666 (+) 702 WP_118854299.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS21_RS17495 proC 3715809..3716633 (+) 825 WP_107683807.1 pyrroline-5-carboxylate reductase -
  QBS21_RS17500 - 3716645..3717196 (+) 552 WP_047436998.1 YggT family protein -
  QBS21_RS17505 yggU 3717196..3717498 (+) 303 WP_107683808.1 DUF167 family protein YggU -
  QBS21_RS17510 - 3717542..3717961 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41080.10 Da        Isoelectric Point: 6.5045

>NTDB_id=814425 QBS21_RS17480 WP_107683852.1 3712753..3713862(-) (pilU) [Aeromonas veronii strain ANYA 19170]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEELQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVSQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814425 QBS21_RS17480 WP_107683852.1 3712753..3713862(-) (pilU) [Aeromonas veronii strain ANYA 19170]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGACGTATCGAGACCCGCATTCCCACCTTTGA
AGAGCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGACTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACATCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTTGATCGCATC
CTCCATCTGGTGTCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCCGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAACTCTCCGGTCGCGAACGGCTCGGCACAGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4N0R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363