Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS21_RS00990 Genome accession   NZ_CP121817
Coordinates   197789..199495 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 19170     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192789..204495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS21_RS00960 mutT 193299..193703 (+) 405 WP_107684321.1 8-oxo-dGTP diphosphatase MutT -
  QBS21_RS00965 yacG 193781..193975 (-) 195 WP_107684320.1 DNA gyrase inhibitor YacG -
  QBS21_RS00970 zapD 193985..194707 (-) 723 WP_005336141.1 cell division protein ZapD -
  QBS21_RS00975 coaE 194745..195359 (-) 615 WP_107684319.1 dephospho-CoA kinase -
  QBS21_RS00980 pilD 195373..196245 (-) 873 WP_107684318.1 A24 family peptidase Machinery gene
  QBS21_RS00985 pilC 196307..197548 (-) 1242 WP_005355091.1 type II secretion system F family protein Machinery gene
  QBS21_RS00990 pilB 197789..199495 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS21_RS00995 - 199499..199963 (-) 465 WP_107684404.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS21_RS01000 nadC 200292..201155 (-) 864 WP_043821973.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS21_RS01005 - 201159..201629 (-) 471 WP_107684317.1 retropepsin-like aspartic protease -
  QBS21_RS01010 ampD 201760..202335 (+) 576 WP_107684316.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS21_RS01015 pdhR 202614..203378 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=814409 QBS21_RS00990 WP_005336134.1 197789..199495(-) (pilB) [Aeromonas veronii strain ANYA 19170]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814409 QBS21_RS00990 WP_005336134.1 197789..199495(-) (pilB) [Aeromonas veronii strain ANYA 19170]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCTCAGCGCTA
TCTGAGCCAGGCCAAGGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAGCACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AACTCACCGCCGCCATCGGTAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCTGATCTCGAAGTCTCCGACGAAAACAGTCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCACCCATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTTTGCATGAAATTGCTACGCCGCCGGTCAATCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTTATGGCACGGCTCGATATCGCCGAACGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAACAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACAGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTAACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTGGAAACTGCCGAAATCGCCATCAAGGCAGCACAAACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCGGCTGC
CGAAACCCTGACCCGAATGATGAACATGGGGGTACCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGCAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTACCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCAGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CGATCGCCCAGAAGGAAGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAAGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377