Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS05_RS01870 Genome accession   NZ_CP121795
Coordinates   354394..356109 (+) Length   571 a.a.
NCBI ID   WP_185450531.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain DAMIAN 12594     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 349394..361109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS05_RS01845 pdhR 350573..351337 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QBS05_RS01850 ampD 351613..352185 (-) 573 WP_208200766.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS05_RS01855 - 352309..352779 (+) 471 WP_010635963.1 retropepsin-like aspartic protease -
  QBS05_RS01860 nadC 352784..353641 (+) 858 WP_094697628.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS05_RS01865 - 353963..354403 (+) 441 WP_010635961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS05_RS01870 pilB 354394..356109 (+) 1716 WP_185450531.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS05_RS01875 pilC 356225..357466 (+) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  QBS05_RS01880 pilD 357489..358361 (+) 873 WP_095591547.1 A24 family peptidase Machinery gene
  QBS05_RS01885 coaE 358381..358995 (+) 615 WP_005305656.1 dephospho-CoA kinase -
  QBS05_RS01890 zapD 359033..359755 (+) 723 WP_010635957.1 cell division protein ZapD -
  QBS05_RS01895 yacG 359765..359959 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  QBS05_RS01900 mutT 360021..360434 (-) 414 WP_279463625.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62871.40 Da        Isoelectric Point: 5.4363

>NTDB_id=814247 QBS05_RS01870 WP_185450531.1 354394..356109(+) (pilB) [Aeromonas dhakensis strain DAMIAN 12594]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEEIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=814247 QBS05_RS01870 WP_185450531.1 354394..356109(+) (pilB) [Aeromonas dhakensis strain DAMIAN 12594]
CTGCCCATAATGACATCTAGTCCCAATAGCGGCCTGGCATTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAAATCCTCGACAGCA
AGGCGCTGGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTACTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAAGCTCTGCTGGTCG
AAGAGAACAAGCTGACCGCCGCCATCAGCAAGCTGATGGAGAGCGATCAGGACGCCCTCGGCATGGAGGAGATCGACGAA
TCCGAGATCTCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCTCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGA
CCAAGTACCGTATTCGTTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGCCTCAAGGTGATGGCGCGCCTGGACATTGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAAT
GTCGCGCAACAAGTCGATGGACATGCGGGTCAACACCCTGCCTACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAGCAGCTCGGCTTTGACGATCGGCAGAAGGCGCAATATCTGCGCGCGCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCT
CAACACCACAGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCA
ACCCCAAGGCTGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTGGTGATGGTCGGTGAA
ATACGGGATCTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAA
CTCGGCGGCCGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCA
TGGCTCAACGACTGGCACGCAAGCTGTGCGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAAGCTGAACTGCTGGAGTTG
GGGTTCACCCAACAGCAGCTGGCAGCAGGCTTGCGGCTGTTCAAGCCGGTTGGCTGCAAGGAGTGTTCGGGCGGCTACAA
GGGGCGGGTCGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAATTCCATGC
AGATTGCGGCCATTGCCCAGAAGGAGGGCATGCGGACCTTGCGTACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

54.783

100

0.552
  pilB Legionella pneumophila strain ERS1305867

54.594

99.124

0.541
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

51.767

99.124

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.424

0.503
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375