Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS29_RS09315 Genome accession   NZ_CP121794
Coordinates   1320816..1322531 (-) Length   571 a.a.
NCBI ID   WP_176722689.1    Uniprot ID   -
Organism   Aeromonas caviae strain FIONA 16800     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1315816..1327531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS29_RS09285 mutT 1316434..1316838 (+) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  QBS29_RS09290 yacG 1316896..1317090 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  QBS29_RS09295 zapD 1317101..1317823 (-) 723 WP_010675825.1 cell division protein ZapD -
  QBS29_RS09300 coaE 1317861..1318475 (-) 615 WP_113978310.1 dephospho-CoA kinase -
  QBS29_RS09305 pilD 1318494..1319369 (-) 876 WP_266109638.1 A24 family peptidase Machinery gene
  QBS29_RS09310 pilC 1319447..1320688 (-) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  QBS29_RS09315 pilB 1320816..1322531 (-) 1716 WP_176722689.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS29_RS09320 - 1322522..1322923 (-) 402 WP_279474298.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS29_RS09325 nadC 1323234..1324097 (-) 864 WP_041213995.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS29_RS09330 - 1324101..1324571 (-) 471 WP_049637199.1 retropepsin-like aspartic protease -
  QBS29_RS09335 ampD 1324700..1325272 (+) 573 WP_039040507.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS29_RS09340 pdhR 1325549..1326313 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62974.47 Da        Isoelectric Point: 5.8214

>NTDB_id=814225 QBS29_RS09315 WP_176722689.1 1320816..1322531(-) (pilB) [Aeromonas caviae strain FIONA 16800]
MYLMTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDE
AEISELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRF
AARLKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRITTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=814225 QBS29_RS09315 WP_176722689.1 1320816..1322531(-) (pilB) [Aeromonas caviae strain FIONA 16800]
CTGTATTTAATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTC
ACAGCGCTATCTGAGCCAGGCTAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCA
AAGCCCTCGCCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCG
CAGAAATACCTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGC
CATGTCCGATCCGACCAATGTGTCGGCTCTGGAGGATTTTGGTTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCG
AAGAGAGCAAGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAG
GCCGAGATCTCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCC
CATCGTCAAATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGA
CCAAGTACCGGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTC
GCGGCTCGCCTCAAGGTGATGGCGCGGCTCGATATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAACTCAAGAT
CTCACGCAACAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGG
ACTCCTCGGCCGCCCGGCTCAATATCGATCAACTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCC
AAACCTCAGGGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCT
CAACACCAGCGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCA
ATCCCAAGGCGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAG
ATACGGGATTTGGAAACCGCCGAGATTGCTATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAA
CTCGGCGGCAGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCA
TGGCCCAGCGCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCAGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTG
GGCTTCACCCAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAG
AGGCCGGGTCGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGC
AGATTGCCGCGATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACC
AGCCTGGCCGAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.009

99.65

0.548
  pilB Acinetobacter baylyi ADP1

55.062

98.599

0.543
  pilB Legionella pneumophila strain ERS1305867

54.867

98.949

0.543
  pilB Vibrio cholerae strain A1552

53.415

100

0.534
  pilB Vibrio campbellii strain DS40M4

50.53

99.124

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

98.599

0.494
  pilF Neisseria gonorrhoeae MS11

46.561

99.299

0.462
  pilF Thermus thermophilus HB27

40.152

92.469

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

96.848

0.368