Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MARGE09_RS01500 Genome accession   NZ_AP023086
Coordinates   413437..415140 (-) Length   567 a.a.
NCBI ID   WP_236985601.1    Uniprot ID   -
Organism   Marinagarivorans cellulosilyticus strain GE09     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408437..420140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARGE09_RS01480 yacG 410524..410718 (-) 195 WP_236985597.1 DNA gyrase inhibitor YacG -
  MARGE09_RS01485 (MARGE09_P0290) coaE 410733..411323 (-) 591 WP_236985598.1 dephospho-CoA kinase -
  MARGE09_RS01490 (MARGE09_P0291) pilD 411329..412216 (-) 888 WP_236985599.1 prepilin peptidase Machinery gene
  MARGE09_RS01495 (MARGE09_P0292) pilC 412216..413433 (-) 1218 WP_236985600.1 type II secretion system F family protein Machinery gene
  MARGE09_RS01500 (MARGE09_P0293) pilB 413437..415140 (-) 1704 WP_236985601.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MARGE09_RS01505 (MARGE09_P0294) - 415388..415711 (-) 324 WP_236985602.1 ComEA family DNA-binding protein -
  MARGE09_RS01510 (MARGE09_P0295) pilA2 416036..416470 (+) 435 WP_236985603.1 pilin Machinery gene
  MARGE09_RS01515 (MARGE09_P0296) - 416514..418397 (+) 1884 WP_236985604.1 O-antigen ligase family protein -
  MARGE09_RS01520 - 418599..418820 (-) 222 WP_236985605.1 hypothetical protein -
  MARGE09_RS01525 (MARGE09_P0297) - 419023..419577 (-) 555 WP_236985606.1 hypothetical protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61929.14 Da        Isoelectric Point: 4.9236

>NTDB_id=81237 MARGE09_RS01500 WP_236985601.1 413437..415140(-) (pilB) [Marinagarivorans cellulosilyticus strain GE09]
MNTPTLSGLTKRLIRDETITSEVAQQATAAAHESKRPLPLCLIDAGAKSLDIAAADEFGSPLLNLGSIELNPALREVVDK
KLVEKHFALPIFKRGNRLFIAVADPTNLHGLDEIKFNTGLSTEAIVVEADKLAVGVEAFLKDSEEDISESLGGLDDDDLD
LELENPDEAKPVEQTNDVDEAPIVKFVNKILLDAIKIGASDIHFEPYEKAYRVRYRTDGILKEVARPPTNLASRLSARLK
VMSQMDISERRIPQDGRIKMKISKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDDQKKLYLDALKQPQGM
ILVTGPTGSGKTVSLYTGLNILNTKEVNISTAEDPVEINLEGINQVNVNTKVGLGFAEALRSFLRQDPDIVMVGEIRDLD
TASIAIKAAQTGHMVMSTLHTNSAPETLTRLLNMGVPTFNVATTVSLIIAQRLARRLCPSCKQPDDIPKPILEEEGMMDI
GIPPEEFELYKPIGCDKCTDGYKGRVGVYEVVRITPAISRIIMEGGNSIEIADAAKKAGFNDLRISALRKTAQGLTSLAE
ANRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=81237 MARGE09_RS01500 WP_236985601.1 413437..415140(-) (pilB) [Marinagarivorans cellulosilyticus strain GE09]
ATGAATACTCCAACACTCAGCGGTCTCACCAAGCGATTGATTCGAGATGAAACCATCACCAGCGAAGTGGCTCAGCAAGC
AACGGCAGCAGCACACGAAAGTAAACGCCCACTCCCCTTGTGCCTTATTGACGCCGGCGCCAAAAGCCTAGACATTGCCG
CTGCCGATGAGTTCGGTTCGCCACTACTAAACTTAGGCAGCATCGAACTAAATCCAGCGCTTAGGGAAGTAGTCGACAAA
AAACTGGTCGAAAAGCACTTTGCCCTACCAATATTCAAGCGCGGAAACCGCCTATTTATTGCAGTAGCAGACCCAACCAA
CCTACATGGCTTGGACGAGATTAAATTCAATACCGGCTTAAGTACAGAAGCTATTGTCGTCGAAGCCGATAAACTCGCCG
TAGGTGTTGAAGCATTCCTGAAGGATTCCGAAGAGGATATTTCCGAAAGCCTTGGCGGGCTTGATGATGACGACCTTGAC
CTCGAGCTTGAAAACCCCGACGAAGCAAAACCCGTCGAACAGACTAACGACGTAGACGAAGCGCCCATTGTTAAATTTGT
GAATAAAATCCTACTGGATGCTATCAAAATAGGCGCTTCCGATATTCACTTCGAACCTTACGAAAAAGCTTATCGCGTTC
GCTACCGCACAGATGGAATACTAAAAGAAGTCGCAAGGCCCCCAACAAACCTTGCATCTAGGCTTTCTGCTCGACTAAAG
GTCATGTCTCAAATGGATATTTCTGAGCGCCGCATTCCGCAGGACGGTCGCATTAAGATGAAAATATCCAAAACCCGCGC
CATCGATTTTCGCGTCAACACGCTTCCTACACTATTTGGCGAAAAGATCGTACTAAGGATTTTAGACCCCTCAAGCGCCA
AGCTTGGCATCGATGCACTAGGCTATGAAGATGATCAGAAGAAGCTTTACCTAGATGCATTGAAACAGCCACAAGGTATG
ATTTTAGTGACAGGTCCTACGGGGTCAGGGAAAACAGTTTCACTTTATACCGGCCTGAATATCCTCAACACTAAAGAAGT
TAATATTTCTACAGCCGAAGACCCTGTAGAAATTAACCTTGAAGGCATTAACCAAGTCAACGTCAACACCAAAGTAGGCC
TTGGTTTCGCGGAAGCTCTTCGTTCATTCTTACGCCAAGATCCCGATATTGTGATGGTAGGGGAGATTCGAGACTTAGAT
ACAGCATCAATTGCCATCAAAGCGGCGCAAACCGGTCACATGGTGATGAGTACACTGCACACCAATAGCGCACCCGAGAC
ATTGACTCGCTTACTCAATATGGGTGTACCGACCTTTAACGTCGCAACAACCGTTAGCCTTATTATTGCTCAGCGCTTGG
CTCGACGCTTATGCCCAAGCTGCAAGCAACCGGACGATATTCCCAAGCCCATCCTTGAAGAGGAAGGCATGATGGATATA
GGCATCCCACCAGAAGAATTTGAGCTTTATAAGCCCATCGGTTGCGATAAGTGCACCGACGGCTATAAAGGTCGTGTCGG
CGTCTATGAAGTTGTTCGCATTACTCCAGCTATTTCTCGCATTATAATGGAGGGCGGCAATTCCATTGAGATTGCTGATG
CCGCAAAGAAAGCTGGCTTTAACGACCTTCGCATATCTGCGCTTCGAAAAACAGCTCAAGGGCTAACCAGCCTTGCAGAA
GCTAACCGTGTTACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(13-133)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.537

100

0.593
  pilB Acinetobacter baumannii D1279779

59.051

100

0.593
  pilB Legionella pneumophila strain ERS1305867

56.736

92.945

0.527
  pilB Vibrio cholerae strain A1552

49.735

99.647

0.496
  pilF Neisseria gonorrhoeae MS11

48.944

100

0.49
  pilB Vibrio campbellii strain DS40M4

53.543

89.594

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

53.333

89.947

0.48

Multiple sequence alignment