Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MARGE09_RS01490 Genome accession   NZ_AP023086
Coordinates   411329..412216 (-) Length   295 a.a.
NCBI ID   WP_236985599.1    Uniprot ID   A0AAN2BIM7
Organism   Marinagarivorans cellulosilyticus strain GE09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406329..417216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARGE09_RS01470 (MARGE09_P0288) moeB 407023..407784 (+) 762 WP_236985595.1 molybdopterin-synthase adenylyltransferase MoeB -
  MARGE09_RS01475 (MARGE09_P0289) - 407791..410292 (-) 2502 WP_236985596.1 TonB-dependent receptor -
  MARGE09_RS01480 yacG 410524..410718 (-) 195 WP_236985597.1 DNA gyrase inhibitor YacG -
  MARGE09_RS01485 (MARGE09_P0290) coaE 410733..411323 (-) 591 WP_236985598.1 dephospho-CoA kinase -
  MARGE09_RS01490 (MARGE09_P0291) pilD 411329..412216 (-) 888 WP_236985599.1 prepilin peptidase Machinery gene
  MARGE09_RS01495 (MARGE09_P0292) pilC 412216..413433 (-) 1218 WP_236985600.1 type II secretion system F family protein Machinery gene
  MARGE09_RS01500 (MARGE09_P0293) pilB 413437..415140 (-) 1704 WP_236985601.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MARGE09_RS01505 (MARGE09_P0294) - 415388..415711 (-) 324 WP_236985602.1 ComEA family DNA-binding protein -
  MARGE09_RS01510 (MARGE09_P0295) pilA2 416036..416470 (+) 435 WP_236985603.1 pilin Machinery gene

Sequence

Protein


Download         Length: 295 a.a.        Molecular weight: 32259.62 Da        Isoelectric Point: 5.7774

>NTDB_id=81235 MARGE09_RS01490 WP_236985599.1 411329..412216(-) (pilD) [Marinagarivorans cellulosilyticus strain GE09]
MSLDQFVSLHQTAPWFTLGVVCILGLVVGSFLNVVIYRIPVMLFKEWKEACDEMIANPAEAPLPEGKFNLAIPNSRCPKC
NSAIKPWQNIPVVSFLILKGACAKCGEKISTRYPLVELFTGIASLMVLWLLGVTPQAAMALILVWALIALTMIDIDHQLL
PDNLTLPLMWLGLLCNLNGLYTDLNSAVIGAMAGYLSLWSVYWLFKLVTGKEGMGFGDFKLLAALGAWMGWEQLPVIIIL
SSLVGAVFGIAGMIMAGKDKEWRIPFGPYLAIAGAIAFFWGDLITEKYLSLSGLS

Nucleotide


Download         Length: 888 bp        

>NTDB_id=81235 MARGE09_RS01490 WP_236985599.1 411329..412216(-) (pilD) [Marinagarivorans cellulosilyticus strain GE09]
ATGTCACTCGACCAATTCGTATCCCTCCATCAAACTGCGCCATGGTTCACTCTTGGCGTTGTTTGCATTTTAGGTTTAGT
TGTCGGTAGTTTTTTAAACGTGGTTATTTATCGCATCCCCGTTATGCTTTTTAAGGAGTGGAAGGAAGCCTGCGATGAGA
TGATTGCGAACCCGGCAGAAGCGCCACTACCAGAAGGTAAGTTCAACCTCGCCATCCCCAACTCTCGCTGCCCTAAATGC
AATAGCGCAATTAAGCCTTGGCAAAACATCCCCGTTGTAAGTTTTTTAATACTTAAAGGCGCCTGCGCTAAATGCGGGGA
GAAGATTTCAACCCGCTATCCGCTAGTCGAGCTATTTACAGGCATTGCAAGCTTGATGGTGTTGTGGCTGCTTGGCGTAA
CGCCGCAGGCCGCTATGGCACTGATATTAGTCTGGGCGTTAATCGCGCTAACAATGATTGATATCGACCACCAGCTGCTA
CCAGATAACCTCACCTTGCCACTTATGTGGCTGGGCCTGCTGTGCAACCTAAACGGTCTTTATACCGATCTAAACAGCGC
TGTTATTGGGGCTATGGCCGGTTATTTAAGCCTTTGGAGCGTGTATTGGTTGTTTAAGCTTGTTACCGGCAAAGAAGGCA
TGGGCTTTGGTGATTTCAAACTACTGGCTGCGCTTGGCGCTTGGATGGGCTGGGAGCAGCTGCCCGTCATTATTATTCTC
TCCTCACTGGTTGGCGCTGTTTTTGGTATTGCAGGCATGATTATGGCTGGCAAAGATAAAGAGTGGCGCATTCCTTTTGG
CCCCTATTTAGCTATCGCCGGTGCTATTGCATTTTTTTGGGGTGACCTAATTACCGAGAAATACCTTAGCCTTTCAGGCT
TAAGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(141-250)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(23-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.439

94.237

0.485
  pilD Acinetobacter nosocomialis M2

51.301

91.186

0.468
  pilD Acinetobacter baumannii D1279779

51.301

91.186

0.468
  pilD Vibrio campbellii strain DS40M4

48.07

96.61

0.464
  pilD Neisseria gonorrhoeae MS11

50.187

90.508

0.454

Multiple sequence alignment