Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Thiosp_RS17925 Genome accession   NZ_CP121473
Coordinates   3964169..3965881 (-) Length   570 a.a.
NCBI ID   WP_201067780.1    Uniprot ID   -
Organism   Thiorhodovibrio litoralis strain 06511     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3959169..3970881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiosp_RS17900 (Thiosp_03622) - 3959301..3960767 (+) 1467 WP_201067775.1 glycosyl hydrolase family 57 -
  Thiosp_RS17905 (Thiosp_03623) - 3961024..3961209 (+) 186 WP_201067776.1 hypothetical protein -
  Thiosp_RS17910 (Thiosp_03624) coaE 3961311..3961958 (-) 648 WP_201067777.1 dephospho-CoA kinase -
  Thiosp_RS17915 (Thiosp_03625) pilD 3961959..3962867 (-) 909 WP_201067778.1 A24 family peptidase Machinery gene
  Thiosp_RS17920 (Thiosp_03626) pilC 3962925..3964163 (-) 1239 WP_201067779.1 type II secretion system F family protein Machinery gene
  Thiosp_RS17925 (Thiosp_03627) pilB 3964169..3965881 (-) 1713 WP_201067780.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Thiosp_RS17930 (Thiosp_03628) - 3966044..3966877 (-) 834 WP_201067794.1 NYN domain-containing protein -
  Thiosp_RS17935 (Thiosp_03629) acs 3967026..3968972 (-) 1947 WP_201067781.1 acetate--CoA ligase -
  Thiosp_RS17940 (Thiosp_03630) dnaJ 3969357..3970505 (-) 1149 WP_201067782.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62659.75 Da        Isoelectric Point: 5.0868

>NTDB_id=812347 Thiosp_RS17925 WP_201067780.1 3964169..3965881(-) (pilB) [Thiorhodovibrio litoralis strain 06511]
MATKQSEVNLSGLARRLIRDELISEETALKIQEESLRRRQPFVQSLVEQKTLDSRTIALAASQEFGVPLLDLNAMDIQTL
PLALVDERLITKHRALPLYRRGNRLFLAISDPTNDQALDEIRFNTGLSAESVLVEEGKLAAALVKAIEGTTTGGVNVSDA
DLENIDIQDPDSETRDDTDANVDDAPIVRYVNKMLVDAISSGTSDIHFEPYEKSFRVRFRQDGMLREVANPPLNIAGRLI
SRLKVMSRMNIAERRVPQDGRIKLKLSRSRDIDFRVNTLPTLYGEKVVLRILDSSAANVGIENLGMDEKQRQDFLESIAK
PYGMILVTGPTGSGKTVSLYSALNILNKPEINISTVEDPVEIQVPGINQVNLNTKGGLTFAAALRAFLRQDPDIVMVGEI
RDLETAEIAVKAAQTGHLVLSTLHTNDAPQSLTRLANMGVPPFNIASSVLMILAQRLARKLCPVCRQAEKIPAEALLEEG
FTQEEVDEGVTIYRPVGCSQCNNGYKGRVGIFQLIKVSEAMGQMIMAGGNSYELADLAAKEGYADLRLSGLRKVKAGVTS
LEELNRITKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=812347 Thiosp_RS17925 WP_201067780.1 3964169..3965881(-) (pilB) [Thiorhodovibrio litoralis strain 06511]
ATGGCGACCAAACAGTCAGAAGTTAATCTCTCAGGGCTCGCGCGCCGACTGATTCGCGACGAGCTGATCTCCGAGGAGAC
GGCGCTCAAGATTCAAGAAGAATCATTGCGCCGGCGCCAGCCGTTCGTGCAGAGCCTGGTCGAGCAAAAGACGCTGGATA
GCCGCACCATCGCCCTGGCGGCCTCCCAGGAATTCGGCGTGCCGCTGCTCGATCTTAACGCGATGGACATCCAAACGCTG
CCGCTCGCTCTGGTCGATGAGCGCTTGATTACCAAGCACCGCGCCCTGCCGCTTTATCGGCGCGGCAACCGGCTGTTCCT
TGCGATCTCCGATCCCACCAACGACCAGGCCCTTGATGAAATTCGCTTCAACACTGGGCTTTCCGCCGAGTCGGTGCTGG
TGGAGGAGGGCAAGCTGGCCGCCGCCTTGGTCAAGGCCATCGAGGGCACGACCACCGGCGGCGTCAATGTCTCGGATGCC
GATCTCGAAAATATCGACATTCAAGACCCCGACAGCGAAACTCGCGACGATACAGACGCCAATGTCGATGACGCCCCCAT
CGTGCGCTATGTCAATAAAATGCTGGTCGATGCCATCTCAAGTGGAACCTCGGATATCCACTTCGAGCCCTACGAGAAGA
GCTTCCGCGTGCGCTTTCGCCAGGATGGGATGCTGCGTGAGGTGGCCAATCCGCCGCTGAATATCGCCGGACGCCTGATT
TCGCGACTGAAGGTGATGTCGCGCATGAATATCGCCGAGCGGCGCGTGCCCCAGGATGGTCGCATCAAGCTCAAGCTATC
GCGCTCACGCGATATTGATTTCCGCGTCAATACCTTGCCCACTCTCTATGGCGAGAAAGTGGTGCTGCGAATTCTCGACT
CCTCCGCGGCCAATGTGGGCATCGAGAACCTCGGCATGGACGAGAAACAGCGCCAGGACTTTTTGGAATCCATCGCCAAA
CCCTACGGCATGATTCTGGTCACTGGACCGACCGGCTCGGGCAAGACGGTCTCGCTCTATTCCGCGCTCAATATTCTCAA
TAAGCCGGAGATCAATATCTCCACCGTCGAGGACCCGGTCGAGATTCAGGTGCCTGGGATCAATCAGGTTAATCTGAACA
CAAAAGGGGGGCTAACCTTCGCCGCGGCCTTGCGCGCCTTTCTGCGTCAGGACCCTGACATCGTCATGGTGGGCGAGATT
CGCGACCTGGAAACCGCCGAGATCGCGGTCAAAGCCGCCCAGACCGGTCACTTGGTGCTCTCTACCCTGCACACCAACGA
TGCTCCCCAGTCCCTGACGCGACTGGCGAACATGGGCGTGCCGCCATTTAATATCGCATCCTCCGTGCTGATGATTTTGG
CCCAGCGCCTGGCGCGAAAGCTCTGCCCCGTGTGTCGTCAGGCCGAGAAGATACCGGCCGAGGCGCTGCTTGAGGAAGGC
TTCACGCAAGAGGAAGTCGACGAAGGTGTGACCATTTACCGCCCGGTGGGCTGCAGTCAGTGCAACAATGGCTACAAAGG
GCGTGTCGGTATTTTTCAGCTCATCAAGGTGTCTGAGGCAATGGGACAAATGATCATGGCAGGGGGTAATTCTTACGAAC
TCGCCGATCTGGCCGCAAAAGAGGGCTATGCCGATTTGCGCCTGTCCGGTCTGCGCAAAGTCAAGGCTGGCGTGACCAGT
CTTGAAGAACTCAATCGGATCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.94

100

0.54
  pilB Legionella pneumophila strain ERS1305867

53.217

100

0.537
  pilB Acinetobacter baylyi ADP1

52.715

100

0.528
  pilF Neisseria gonorrhoeae MS11

47.331

98.596

0.467
  pilB Vibrio campbellii strain DS40M4

49.165

94.561

0.465
  pilB Vibrio cholerae strain A1552

49.811

92.632

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

49.242

92.632

0.456
  pilF Thermus thermophilus HB27

40.797

92.456

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.7

93.684

0.372