Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MARGE09_RS01375 Genome accession   NZ_AP023086
Coordinates   381985..384162 (+) Length   725 a.a.
NCBI ID   WP_236985578.1    Uniprot ID   A0AAN1WEE8
Organism   Marinagarivorans cellulosilyticus strain GE09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 376985..389162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARGE09_RS01355 (MARGE09_P0266) pilM 379185..380252 (+) 1068 WP_236985574.1 pilus assembly protein PilM Machinery gene
  MARGE09_RS01360 (MARGE09_P0267) - 380254..380820 (+) 567 WP_236985575.1 PilN domain-containing protein -
  MARGE09_RS01365 (MARGE09_P0268) - 380832..381449 (+) 618 WP_236985576.1 type 4a pilus biogenesis protein PilO -
  MARGE09_RS01370 (MARGE09_P0269) - 381455..381979 (+) 525 WP_236985577.1 pilus assembly protein PilP -
  MARGE09_RS01375 (MARGE09_P0270) pilQ 381985..384162 (+) 2178 WP_236985578.1 type IV pilus secretin PilQ family protein Machinery gene
  MARGE09_RS01380 (MARGE09_P0271) - 384162..384680 (+) 519 WP_236985579.1 shikimate kinase -
  MARGE09_RS01385 (MARGE09_P0272) aroB 384768..385865 (+) 1098 WP_236985580.1 3-dehydroquinate synthase -
  MARGE09_RS01390 (MARGE09_P0273) - 385907..387415 (+) 1509 WP_236985581.1 AAA family ATPase -

Sequence

Protein


Download         Length: 725 a.a.        Molecular weight: 78929.26 Da        Isoelectric Point: 4.3899

>NTDB_id=81234 MARGE09_RS01375 WP_236985578.1 381985..384162(+) (pilQ) [Marinagarivorans cellulosilyticus strain GE09]
MFTKYRKIAGVLSGLMMTPLAWSSELQDVKFAELPGDRVEIRLVFDEAPSSPQGYTIEQPARIVLDFADVENALAQKKFN
MDMGSVSSAVVVAGGNRTRLIVNMNTLEPYATRVEGTDLVMEVGGSAKALAAVEPQSTVATAASPVLAKPKNRFDKSVSE
IQNIDFRRGDAGEGKVIVQLSNPGVSIDVEEVAGGIEVGFLQTDLPDALRRKLDVTDFATPVQMIDATTGSLRTVLKIDA
NGEYDYLAYQADNEYVVSIKPLTKAEIEEKRAKFAYTGEKLSLNFQNIEVRSVLQLIADFTDLNLVASDTVKGSITLRLD
NVPWDQALELVLKTKGLDKRQVGNVLMVAPAAEIAERERQELATQKQLEELAPLRTEYIRVRYANARELFELFIPEGGLG
GQSGSGGNQRNSTGTILSDRGHAIVDERTNSIILTDTEEKINDFRRVIEQIDVPIRQVMIEARIVIANNNFRKELGSRIN
FASQSRFKNGQIGYSGKMDELFNNSNGEAGTVYSLGDNDPDDYGETITDVDSRAVDLGVIESFGNFTMGVVSGDVLLGLE
ISALEESGFAEIVSQPKVITGDKQQASIESGTEIAYQEASASGATTVAFKEAVLKLDVTPQITPDDNIIMDLKINQDNVG
ELTNAGIPTIDVTELNTQVLVADGQTVVLGGIFQNNVVTGQSKVPLLGDIPVLGRLFRKDTKSEDKKELIIFVTPRIMSS
TLTNN

Nucleotide


Download         Length: 2178 bp        

>NTDB_id=81234 MARGE09_RS01375 WP_236985578.1 381985..384162(+) (pilQ) [Marinagarivorans cellulosilyticus strain GE09]
ATGTTCACAAAATACCGAAAAATTGCTGGAGTTCTATCCGGTTTAATGATGACTCCACTTGCTTGGTCAAGTGAGCTTCA
AGATGTCAAGTTTGCTGAGCTGCCAGGTGATCGCGTTGAAATCCGCTTGGTGTTTGATGAGGCGCCATCTAGCCCGCAAG
GCTATACCATTGAGCAGCCTGCTCGCATCGTGCTGGACTTTGCTGATGTTGAAAACGCACTAGCGCAGAAAAAGTTTAAT
ATGGATATGGGAAGCGTAAGTAGCGCTGTTGTGGTAGCTGGCGGCAACCGTACTCGGCTGATTGTTAATATGAACACCCT
GGAGCCATACGCCACGCGAGTTGAAGGTACTGATCTTGTTATGGAAGTAGGTGGCTCTGCAAAAGCCTTGGCTGCGGTTG
AGCCTCAGTCTACTGTTGCAACGGCAGCTTCGCCTGTATTGGCCAAGCCTAAGAATCGCTTCGATAAATCCGTTTCTGAA
ATTCAGAATATTGATTTTCGTCGTGGCGATGCTGGCGAGGGCAAAGTCATTGTTCAGCTCAGCAATCCAGGTGTAAGTAT
TGATGTTGAAGAGGTTGCCGGTGGTATTGAGGTTGGCTTCTTACAGACCGACTTGCCTGACGCGCTGCGCAGGAAGCTAG
ATGTCACTGATTTCGCAACGCCGGTGCAGATGATTGATGCAACTACTGGCAGCTTGCGTACAGTGTTAAAAATTGATGCC
AATGGCGAATATGACTACTTGGCCTATCAAGCCGACAATGAATATGTTGTTAGTATTAAGCCGCTAACGAAGGCAGAGAT
AGAAGAGAAACGCGCTAAATTTGCTTATACAGGTGAAAAACTGTCGCTTAACTTCCAAAACATCGAAGTACGCTCTGTAT
TGCAGTTAATTGCTGATTTTACCGATCTAAACCTAGTGGCAAGTGACACTGTTAAAGGCAGCATTACTTTACGCTTAGAT
AACGTGCCTTGGGATCAGGCATTGGAGCTGGTGCTAAAAACTAAGGGCTTGGATAAGCGCCAGGTGGGTAATGTTTTGAT
GGTGGCTCCAGCGGCAGAAATTGCAGAGCGTGAGCGCCAAGAGCTGGCTACTCAGAAACAGTTAGAAGAGTTAGCGCCAT
TACGTACGGAGTATATTCGCGTACGCTACGCCAATGCTCGAGAATTATTCGAGTTGTTTATCCCAGAAGGTGGTCTTGGT
GGGCAGTCCGGTAGTGGCGGTAATCAAAGAAACTCAACGGGCACTATTTTGTCTGATCGGGGGCATGCAATTGTTGATGA
GCGTACTAACTCAATTATTTTGACGGATACTGAAGAGAAGATTAATGATTTTCGTCGAGTAATTGAGCAAATTGATGTGC
CTATTAGGCAGGTTATGATTGAAGCTCGTATTGTAATTGCTAATAATAACTTCAGAAAAGAGTTAGGCTCGAGAATAAAC
TTTGCCTCCCAGAGTCGTTTCAAAAATGGACAAATAGGCTATAGCGGGAAAATGGATGAACTGTTTAATAACTCTAATGG
TGAGGCGGGCACTGTTTATAGTTTGGGTGACAATGACCCTGATGATTATGGTGAGACAATAACTGATGTTGATTCAAGAG
CTGTGGATCTAGGAGTTATTGAGTCCTTTGGCAATTTTACGATGGGAGTGGTATCTGGCGATGTATTGCTGGGACTTGAG
ATTTCAGCTCTTGAAGAAAGTGGGTTCGCTGAGATTGTGAGTCAACCTAAAGTAATCACCGGTGATAAGCAGCAAGCCTC
GATTGAAAGCGGTACCGAAATCGCTTATCAAGAGGCTTCTGCAAGTGGGGCAACTACGGTTGCATTCAAAGAGGCAGTAT
TGAAGCTTGATGTTACTCCGCAAATTACGCCCGATGACAACATCATTATGGATCTTAAGATTAACCAAGATAATGTGGGT
GAGCTGACCAATGCTGGTATTCCGACGATTGATGTGACTGAGCTAAATACGCAGGTATTGGTTGCTGATGGTCAAACGGT
AGTGTTAGGTGGTATTTTCCAAAATAACGTTGTGACTGGGCAATCTAAAGTTCCTTTGCTGGGTGATATCCCCGTGCTTG
GTCGTCTGTTCCGTAAAGATACCAAGAGCGAGGATAAGAAAGAGCTCATCATTTTTGTTACCCCCCGTATTATGTCGAGC
ACACTGACGAATAACTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(304-350)

AMIN domain (AMIN)

(37-109)

Bacterial type II and III secretion system protein (Secretin)

(563-718)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.974

100

0.494
  pilQ Acinetobacter baumannii strain A118

40.167

98.897

0.397
  pilQ Acinetobacter baumannii D1279779

40.167

98.897

0.397
  comQ Acinetobacter baylyi ADP1

40.709

97.241

0.396
  pilQ Legionella pneumophila strain Lp02

37.781

98.207

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.96

97.379

0.37

Multiple sequence alignment