Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MARGE09_RS01355 Genome accession   NZ_AP023086
Coordinates   379185..380252 (+) Length   355 a.a.
NCBI ID   WP_236985574.1    Uniprot ID   A0AAN1WEF9
Organism   Marinagarivorans cellulosilyticus strain GE09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 374185..385252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARGE09_RS01345 (MARGE09_P0264) htpX 375706..376590 (+) 885 WP_236985572.1 protease HtpX -
  MARGE09_RS01350 (MARGE09_P0265) - 376587..379013 (-) 2427 WP_236985573.1 penicillin-binding protein 1A -
  MARGE09_RS01355 (MARGE09_P0266) pilM 379185..380252 (+) 1068 WP_236985574.1 pilus assembly protein PilM Machinery gene
  MARGE09_RS01360 (MARGE09_P0267) - 380254..380820 (+) 567 WP_236985575.1 PilN domain-containing protein -
  MARGE09_RS01365 (MARGE09_P0268) - 380832..381449 (+) 618 WP_236985576.1 type 4a pilus biogenesis protein PilO -
  MARGE09_RS01370 (MARGE09_P0269) - 381455..381979 (+) 525 WP_236985577.1 pilus assembly protein PilP -
  MARGE09_RS01375 (MARGE09_P0270) pilQ 381985..384162 (+) 2178 WP_236985578.1 type IV pilus secretin PilQ family protein Machinery gene
  MARGE09_RS01380 (MARGE09_P0271) - 384162..384680 (+) 519 WP_236985579.1 shikimate kinase -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38537.74 Da        Isoelectric Point: 4.2814

>NTDB_id=81233 MARGE09_RS01355 WP_236985574.1 379185..380252(+) (pilM) [Marinagarivorans cellulosilyticus strain GE09]
MSVLSFLSKKSKPLLGLDISSTTVKMIELTKHGDSYRVENYSVRPLPPGVVVEKNISDVDAVAQVVKATIQQARTKVRDV
ALAVPGSAVITKTIEMPAGLTDDAMELQISLEADQYIPYPIEEVSIDFDVLGPSEKSPEQVNVLLAACRSENVETREAVM
ELAGLTAKVVDVEAYALERSFDLQADQIDGAYDKVIAIVDIGSTMTTLSVLVEGKIVYAREQLFGGKQLTEEIQRRYGLS
EEEAGLAKRQGGLPDDYEAEVLEPFKDSVVQQVTRSLQFFFSSSQYHEVDHVLLAGGVASMDGLSDMVEDKLETHTTVAN
PFANMSVSSRVNSAALANDAPSLMIVTGLALRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=81233 MARGE09_RS01355 WP_236985574.1 379185..380252(+) (pilM) [Marinagarivorans cellulosilyticus strain GE09]
ATGAGTGTTCTCAGCTTTCTAAGCAAAAAAAGCAAGCCGTTGCTCGGTTTGGATATCAGCTCGACGACAGTAAAAATGAT
AGAGCTCACCAAGCATGGCGATAGCTATCGTGTGGAGAACTACTCTGTGAGGCCGCTGCCTCCGGGGGTTGTCGTTGAAA
AAAATATCAGTGATGTCGATGCTGTCGCGCAGGTTGTGAAAGCAACGATTCAGCAAGCGCGTACCAAAGTGCGTGATGTC
GCACTGGCAGTGCCCGGTTCGGCTGTTATCACAAAAACCATCGAAATGCCGGCTGGTTTAACTGACGACGCAATGGAATT
GCAAATTTCATTGGAGGCTGATCAGTATATTCCTTACCCTATAGAAGAGGTTTCAATCGATTTCGATGTTCTCGGCCCTT
CTGAAAAATCTCCTGAACAAGTCAACGTTCTGCTAGCGGCTTGCCGTAGTGAAAATGTTGAGACACGTGAAGCGGTTATG
GAGTTGGCTGGGCTTACTGCAAAAGTTGTCGATGTTGAAGCTTATGCCCTTGAGCGTTCTTTTGATTTGCAAGCAGACCA
AATTGACGGAGCCTACGATAAAGTGATTGCTATTGTTGATATAGGGTCAACAATGACTACGCTGAGTGTATTGGTTGAGG
GCAAGATTGTTTATGCTCGCGAGCAGTTATTTGGCGGCAAGCAGCTTACAGAGGAAATTCAGCGCCGTTATGGCTTGTCT
GAAGAGGAGGCTGGTTTAGCTAAGCGTCAAGGGGGCTTGCCTGATGATTACGAGGCTGAGGTGTTGGAACCCTTTAAAGA
TAGTGTGGTTCAGCAGGTTACGCGCTCGTTGCAGTTTTTCTTCTCCTCGAGCCAGTACCATGAGGTCGATCATGTTTTGT
TAGCCGGTGGCGTTGCTTCAATGGATGGTTTATCTGACATGGTGGAAGATAAGCTTGAAACCCACACAACGGTTGCAAAC
CCTTTTGCTAATATGTCGGTGTCTTCGAGAGTGAATTCTGCTGCATTGGCTAATGATGCTCCTTCATTAATGATTGTGAC
GGGCTTGGCGTTAAGGAGCTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.085

99.718

0.549
  comM Acinetobacter nosocomialis M2

55.085

99.718

0.549
  comM Acinetobacter baylyi ADP1

52.975

99.437

0.527
  pilM Legionella pneumophila strain ERS1305867

46.045

99.718

0.459

Multiple sequence alignment